AB187645

Anti-Proteasome 20S LMP2 antibody [EPR13784]

RabMAb
Recombinant
KO Validated
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(2 Publications)

Rabbit Recombinant Monoclonal Proteasome 20S LMP2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

LMP2, PSMB6i, RING12, PSMB9, Proteasome subunit beta type-9, Low molecular mass protein 2, Macropain chain 7, Multicatalytic endopeptidase complex chain 7, Proteasome chain 7, Proteasome subunit beta-1i, Really interesting new gene 12 protein

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling Proteasome 20S LMP2 with ab187645 at 1/100 dilution. Counter stained with Dapi (blue).

Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedRaji cells labeling Proteasome 20S LMP2 with ab187645 at 1/170 dilution compared to a Rabbit monoclonal IgG isotype control, followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

Western blot analysis of Proteasome 20S LMP2 in Raji cell lysate immunoprecipitated using ab187645 at 1/50 (Lane 1). Lane 2 : PBS instead of Raji lysate.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

Based on the sequence analysis by BLAST, ab187645 recognizes two isoforms with the predicted MWs of 22kDa and 23kDa respectively.

All lanes:

Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

Predicted band size: 23 kDa

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Lab

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Proteasome 20S LMP2 knockout HAP1 cell lysate (20 μg)
Lanes 1 - 2 : Merged signal (red and green). Green - ab187645 observed at 22 and 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab187645 was shown to recognize Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab187645 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

Predicted band size: 23 kDa

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Supplier Data

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

All lanes:

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645) at 1/2000 dilution

Lane 1:

HL-60 cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 21 kDa

false

Supplier Data

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

All lanes:

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645) at 1/10000 dilution

Lane 1:

Ramos cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 21 kDa

false

Carrier free

Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13784

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/170", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The proteasome LMP2 also known as proteasome subunit beta type-1 or PSMB9 is a component important in the functioning of the 20S proteasome complex. This molecular entity with a mass of approximately 23 kDa is predominantly expressed in immune cells including lymphocytes. LMP2 incorporates into the immunoproteasome a specialized variant of the proteasome that participates prominently in the processing of class I MHC peptides enhancing the immune response.

Biological function summary

LMP2 is integral to protein catabolism and regulation of the cellular protein homeostasis. As part of the larger proteasome complex the LMP2 protein functions in degradative pathways that recycle amino acids from ubiquitin-tagged proteins. This proteasome takes part in antigen processing helping convert proteins into peptides that are presented on the surface of cells for immune system recognition.

Pathways

LMP2 is significantly involved in the ubiquitin-proteasome pathway and immune system processes. It interacts with other immunoproteasome subunits like LMP7 and MECL-1 which work together to enhance the proteolytic capacity for antigen presentation. The presence of LMP2 in these pathways demonstrates its role in adaptive immunity contributing to the clearance of misfolded or damaged proteins and therefore maintaining cell health.

LMP2 associates with immune-related conditions and some cancer types. Dysregulation of LMP2 expression or function can impact antigen presentation potentially leading to autoimmune disorders due to inappropriate immune activation. Moreover alterations in LMP2 expression have been observed in certain cancers such as melanoma where it affects proteasome activity and influences tumor progression. In these contexts interactions with proteins like TAP1 and TAP2 which aid in peptide transport mark its relevance in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH (PubMed : 33727065, PubMed : 34819510). The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.

See full target information PSMB9

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports methods 4:100903 PubMed39561715

2024

Accelerated production of human epithelial organoids in a miniaturized spinning bioreactor.

Applications

Unspecified application

Species

Unspecified reactive species

Shicheng Ye,Ary Marsee,Gilles S van Tienderen,Mohammad Rezaeimoghaddam,Hafsah Sheikh,Roos-Anne Samsom,Eelco J P de Koning,Sabine Fuchs,Monique M A Verstegen,Luc J W van der Laan,Frans van de Vosse,Jos Malda,Keita Ito,Bart Spee,Kerstin Schneeberger

Environmental toxicology 37:2103-2114 PubMed35506645

2022

Transcriptome-wide association study identifies PSMB9 as a susceptibility gene for coal workers' pneumoconiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiali Yuan,Ting Wang,Lijuan Wang,Ping Li,Hongbing Shen,Yiqun Mo,Qunwei Zhang,Chunhui Ni

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome 20S LMP2 antibody [EPR13784]

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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