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Rabbit Recombinant Monoclonal Proteasome 20S LMP2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645), expandable thumbnail
  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645), expandable thumbnail
  • Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645), expandable thumbnail
  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP2 antibody [EPR13784] (AB187645), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30 - 1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/170
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH (PubMed:33727065, PubMed:34819510). The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.

Alternative names

LMP2, PSMB6i, RING12, PSMB9, Proteasome subunit beta type-9, Low molecular mass protein 2, Macropain chain 7, Multicatalytic endopeptidase complex chain 7, Proteasome chain 7, Proteasome subunit beta-1i, Really interesting new gene 12 protein

Recommended products

Rabbit Recombinant Monoclonal Proteasome 20S LMP2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13784
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The proteasome LMP2 also known as proteasome subunit beta type-1 or PSMB9 is a component important in the functioning of the 20S proteasome complex. This molecular entity with a mass of approximately 23 kDa is predominantly expressed in immune cells including lymphocytes. LMP2 incorporates into the immunoproteasome a specialized variant of the proteasome that participates prominently in the processing of class I MHC peptides enhancing the immune response.

Biological function summary

LMP2 is integral to protein catabolism and regulation of the cellular protein homeostasis. As part of the larger proteasome complex the LMP2 protein functions in degradative pathways that recycle amino acids from ubiquitin-tagged proteins. This proteasome takes part in antigen processing helping convert proteins into peptides that are presented on the surface of cells for immune system recognition.

Pathways

LMP2 is significantly involved in the ubiquitin-proteasome pathway and immune system processes. It interacts with other immunoproteasome subunits like LMP7 and MECL-1 which work together to enhance the proteolytic capacity for antigen presentation. The presence of LMP2 in these pathways demonstrates its role in adaptive immunity contributing to the clearance of misfolded or damaged proteins and therefore maintaining cell health.

Associated diseases and disorders

LMP2 associates with immune-related conditions and some cancer types. Dysregulation of LMP2 expression or function can impact antigen presentation potentially leading to autoimmune disorders due to inappropriate immune activation. Moreover alterations in LMP2 expression have been observed in certain cancers such as melanoma where it affects proteasome activity and influences tumor progression. In these contexts interactions with proteins like TAP1 and TAP2 which aid in peptide transport mark its relevance in disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645), expandable thumbnail

    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Proteasome 20S LMP2 knockout HAP1 cell lysate (20 μg)
    Lanes 1 - 2: Merged signal (red and green). Green - ab187645 observed at 22 and 24 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
    ab187645 was shown to recognize Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab187645 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

    Predicted band size: 23 kDa

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645), expandable thumbnail

    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

    All lanes: Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645) at 1/10000 dilution

    Lane 1: Ramos cell lysate at 20 µg

    Lane 2: Raji cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 23 kDa

    Observed band size: 21 kDa

  • Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645), expandable thumbnail

    Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

    Western blot analysis of Proteasome 20S LMP2 in Raji cell lysate immunoprecipitated using ab187645 at 1/50 (Lane 1). Lane 2: PBS instead of Raji lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    Based on the sequence analysis by BLAST, ab187645 recognizes two isoforms with the predicted MWs of 22kDa and 23kDa respectively.

    All lanes: Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

    Predicted band size: 23 kDa

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645), expandable thumbnail

    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

    All lanes: Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645) at 1/2000 dilution

    Lane 1: HL-60 cell lysate at 20 µg

    Lane 2: A431 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 23 kDa

    Observed band size: 21 kDa

  • Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedRaji cells labeling Proteasome 20S LMP2 with ab187645 at 1/170 dilution compared to a Rabbit monoclonal IgG isotype control, followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling Proteasome 20S LMP2 with ab187645 at 1/100 dilution. Counter stained with Dapi (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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