Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (AB250928)

This data was developed using ab187645, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling Proteasome 20S LMP2 with ab187645 at 1/100 dilution. Counter stained with Dapi (blue).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (AB250928)

This data was developed using ab187645, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Raji cells labeling Proteasome 20S LMP2 with ab187645 at 1/170 dilution compared to a Rabbit monoclonal IgG isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (AB250928)

This data was developed using ab187645, the same antibody clone in a different buffer formulation.

Western blot analysis of Proteasome 20S LMP2 in Raji cell lysate immunoprecipitated using ab187645 at 1/50 (Lane 1). Lane 2 : PBS instead of Raji lysate.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

Based on the sequence analysis by BLAST, ab187645 recognizes two isoforms with the predicted MWs of 22kDa and 23kDa respectively.

All lanes:

Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] (<a href='/en-us/products/primary-antibodies/proteasome-20s-lmp2-antibody-epr13784-ab187645'>ab187645</a>)

Predicted band size: 23 kDa

false

• WB

Lab

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (AB250928)

This data was developed using ab187645, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 Âμg)

Lane 2 : Proteasome 20S LMP2 knockout HAP1 cell lysate (20 Âμg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab187645 observed at 22 and 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab187645 was shown to recognize Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab187645 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (<a href='/en-us/products/primary-antibodies/proteasome-20s-lmp2-antibody-epr13784-ab187645'>ab187645</a>)

Predicted band size: 23 kDa

false

• WB

Supplier Data

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (AB250928)

This data was developed using ab187645, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (<a href='/en-us/products/primary-antibodies/proteasome-20s-lmp2-antibody-epr13784-ab187645'>ab187645</a>) at 1/2000 dilution

Lane 1:

HL-60 cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (AB250928)

This data was developed using ab187645, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (<a href='/en-us/products/primary-antibodies/proteasome-20s-lmp2-antibody-epr13784-ab187645'>ab187645</a>) at 1/10000 dilution

Lane 1:

Ramos cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 21 kDa

false