Rabbit Polyclonal Proteasome 20S LMP7 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 54 publications. Immunogen corresponding to Synthetic Peptide within Human PSMB8 aa 250 to C-terminus.
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- Molecular cancer 22:1962023Immunoproteasome function maintains oncogenic gene expression in KMT2A-complex driven leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNuria Tubío-Santamaría,Ashok Kumar Jayavelu,Tina M Schnoeder,Theresa Eifert,Chen-Jen Hsu,Florian Perner,Qirui Zhang,Daniela V Wenge,Fynn M Hansen,Joanna M Kirkpatrick,Nidhi Jyotsana,Steven W Lane,Björn von Eyss,Aniruddha J Deshpande,Michael W M Kühn,Juerg Schwaller,Clemens Cammann,Ulrike Seifert,Frédéric Ebstein,Elke Krüger,Andreas Hochhaus,Michael Heuser,Alessandro Ori,Matthias Mann,Scott A Armstrong,Florian H HeidelPubMed 38049829
- International journal of molecular sciences 24:2023Ubiquitin Proteasome System Role in Diabetes-Induced Cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOrtal Nahum-Ankonina,Efrat Kurtzwald-Josefson,Aaron Ciechanover,Maayan Waldman,Orna Shwartz-Rohaker,Edith Hochhauser,Sam J Meyer,Dan Aravot,Moshe Phillip,Yaron D BaracPubMed 37895057
- Nature communications 14:40922023Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinji Kim,Remigiusz A Serwa,Lukasz Samluk,Ida Suppanz,Agata Kodroń,Tomasz M Stępkowski,Praveenraj Elancheliyan,Biniyam Tsegaye,Silke Oeljeklaus,Michal Wasilewski,Bettina Warscheid,Agnieszka ChacinskaPubMed 37433777
- iScience 26:1069092023Integrative systems biology characterizes immune-mediated neurodevelopmental changes in murine Zika virus microcephaly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKimino Fujimura,Amanda J Guise,Tojo Nakayama,Christoph N Schlaffner,Anais Meziani,Mukesh Kumar,Long Cheng,Dylan J Vaughan,Andrew Kodani,Simon Van Haren,Kenneth Parker,Ofer Levy,Ann F Durbin,Irene Bosch,Lee Gehrke,Hanno Steen,Ganeshwaran H Mochida,Judith A SteenPubMed 37332674
- Aging 15:3295-33302023Proteostatic modulation in brain aging without associated Alzheimer's disease-and age-related neuropathological changes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPol Andrés-Benito,Ignacio Íñigo-Marco,Marta Brullas,Margarita Carmona,José Antonio Del Rio,Joaquín Fernández-Irigoyen,Enrique Santamaría,Mónica Povedano,Isidro FerrerPubMed 37179123
- The EMBO journal 42:e1105972023DNA sensing via the cGAS/STING pathway activates the immunoproteasome and adaptive T-cell immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyuan Wang,Huabin Zhang,Yuqin Wang,Laylan Bramasole,Kai Guo,Fatima Mourtada,Thomas Meul,Qianjiang Hu,Valeria Viteri,Ilona Kammerl,Melanie Konigshoff,Mareike Lehmann,Thomas Magg,Fabian Hauck,Isis E Fernandez,Silke MeinersPubMed 36912165
- EMBO molecular medicine 15:e162442022Microbiota dysbiosis influences immune system and muscle pathophysiology of dystrophin-deficient mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Farini,Luana Tripodi,Chiara Villa,Francesco Strati,Amanda Facoetti,Guido Baselli,Jacopo Troisi,Annamaria Landolfi,Caterina Lonati,Davide Molinaro,Michelle Wintzinger,Stefano Gatti,Barbara Cassani,Flavio Caprioli,Federica Facciotti,Mattia Quattrocelli,Yvan TorrentePubMed 36533294
- International journal of molecular sciences 23:2022Immunoproteasome Inhibition Ameliorates Aged Dystrophic Mouse Muscle Environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuana Tripodi,Davide Molinaro,Francesco Fortunato,Carolina Mella,Barbara Cassani,Yvan Torrente,Andrea FariniPubMed 36498987
- Traffic (Copenhagen, Denmark) 23:287-3042022Extracellular 20S proteasome secreted via microvesicles can degrade poorly folded proteins and inhibit Galectin-3 agglutination activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Bonhoure,Laurent Henry,Claudia Bich,Lionel Blanc,Blanche Bergeret,Marie-Pierre Bousquet,Olivier Coux,Pierre-Emmanuel Stoebner,Michel VidalPubMed 35466519
- The European respiratory journal 59:2022Activation of immune cell proteasomes in peripheral blood of smokers and COPD patients: implications for therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlona E Kammerl,Sophie Hardy,Claudia Flexeder,Andrea Urmann,Julia Peierl,Yuqin Wang,Oliver Vosyka,Marion Frankenberger,Katrin Milger,Jürgen Behr,Andrea Koch,Juliane Merl-Pham,Stefanie M Hauck,Charles Pilette,Holger Schulz,Silke MeinersPubMed 34561290
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 99% PBS, 0.1% BSA
- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human PSMB8 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P28062
Consider this alternative
Reactivity data
Select an application| ICC | WB | |
|---|---|---|
| Human | Tested | Tested |
| Cow | Predicted | Predicted |
| Sheep | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-3 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Involved in the generation of spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein (PubMed:27049119). Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
Alternative names
LMP7, PSMB5i, RING10, Y2, PSMB8, Proteasome subunit beta type-8, Low molecular mass protein 7, Macropain subunit C13, Multicatalytic endopeptidase complex subunit C13, Proteasome component C13, Proteasome subunit beta-5i, Really interesting new gene 10 protein
Recommended products
Rabbit Polyclonal Proteasome 20S LMP7 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 54 publications. Immunogen corresponding to Synthetic Peptide within Human PSMB8 aa 250 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 99% PBS, 0.1% BSA
- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human PSMB8 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P28062
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Proteasome 20S LMP7 also known as β5i or PSMB8 is a subunit of the immunoproteasome. It weighs about 23 kDa and it is expressed predominantly in immune cells such as lymphocytes and macrophages. LMP7 is part of the proteasome 20S a cylindrical core particle involved in the degradation of ubiquitinated proteins. This process helps maintain protein homeostasis by breaking down misfolded and damaged proteins.
- Biological function summary
The immunoproteasome in which LMP7 is an essential component plays an important role in the adaptive immune system. It influences the generation of antigenic peptides that are presented on MHC class I molecules. LMP7's expression is increased during immune responses particularly following gamma interferon (IFN-γ) stimulation contributing to the immune-mediated proteasome conformation. The immunoproteasome enables more efficient peptide processing enhancing antigen presentation to cytotoxic T lymphocytes.
- Pathways
LMP7 is involved in the MHC class I antigen presentation pathway. This pathway allows the immune system to detect and respond to intracellular pathogens such as viruses. Additionally LMP7 interacts with TAP (Transporter associated with Antigen Processing) and other proteasome subunits to ensure effective peptide processing for loading onto MHC class I molecules. Through this involvement it closely links to immune signaling and cellular stress responses.
- Associated diseases and disorders
Research shows that LMP7 connects to autoimmune diseases and inflammatory disorders such as lupus and rheumatoid arthritis. Dysregulation of LMP7 expression or activity can lead to improper immune responses contributing to disease pathogenesis. Furthermore LMP7 impacts the degradation of specific proteins relevant to these conditions. For example it works alongside other proteasome subunits like LMP2 and MECL-1 in modulating the inflammatory response which can exacerbate autoimmune disease symptoms.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunocytochemistry - Anti-Proteasome 20S LMP7 antibody (ab3329)
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Proteasome 20S LMP7 with ab3329 at 5μg/ml. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA in PBS for 15 minutes at room temperature. Cells were stained with or without Anti-Proteasome 20S LMP7 antibody (ab3329), at a concentration of 5μg/ml for 1 hour at room temperature, and then incubated with a Alexa Fluor® 488 goat anti-rabbit IgG secondary antibody at a dilution of 1/1000 for 1 hour s at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye.
Western blot - Anti-Proteasome 20S LMP7 antibody (ab3329)
Detected by chemiluminescence.
All lanes: Western blot - Anti-Proteasome 20S LMP7 antibody (ab3329) at 2 µg/mL
Lane 1: HeLa whole cell extracts at 30 µg
Lane 2: HeLa treated with IFN gamma (100ng/ml IFN gamma for 72h) whole cell extracts at 30 µg
Lane 3: U-937 whole cell extracts at 30 µg
Lane 4: Raji whole cell extracts at 30 µg
Lane 5: Ramos whole cell extracts at 30 µg
SecondaryAll lanes: Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution
Predicted band size: 30 kDa
Observed band size: 20 kDa
Immunocytochemistry - Anti-Proteasome 20S LMP7 antibody (ab3329)
ICC/IF image of ab3329 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3329, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-Proteasome 20S LMP7 antibody (ab3329)
Western blot of proteasome 20S LMP7 from HeLa cell extract using ab3329.
All lanes: Western blot - Anti-Proteasome 20S LMP7 antibody (ab3329)
Predicted band size: 30 kDa
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