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AB180606

Anti-Proteasome 20S LMP7 antibody [EPR14482(B)]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

5

(1 Review)

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(12 Publications)

Rabbit Recombinant Monoclonal Proteasome 20S LMP7 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications.

View Alternative Names

LMP7, PSMB5i, RING10, Y2, PSMB8, Proteasome subunit beta type-8, Low molecular mass protein 7, Macropain subunit C13, Multicatalytic endopeptidase complex subunit C13, Proteasome component C13, Proteasome subunit beta-5i, Really interesting new gene 10 protein

10 Images
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)

ab180606 (unpurified) staining Proteasome 20S LMP7 in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Negative control : PBS only.

Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedJurkat (Human T cell leukemia cell line from peripheral blood) cells labeling Proteasome 20S LMP7 with ab180606 (unpurified) at 1/150 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)

Immunofluorecenct analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Proteasome 20S LMP7 with ab180606 (unpurified) at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (left panel). DAPI staining (right panel).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)

Immunohistochemical analysis of paraffin-embedded human bladder transitional cell carcinoma tissue labeling Proteasome 20S LMP7 with ab180606 (unpurified) at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue sections labeling Proteasome 20S LMP7 with purified ab180606 at 1/1700 dilution (0.52 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Proteasome 20S LMP7 with purified ab180606 at 1 : 100 dilution (8.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)
  • WB

Lab

Western blot - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)

Lanes 1-4 : Merged signal (red and green). Green - ab180606 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.

ab180606 Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] was shown to specifically react with Proteasome 20S LMP7 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267148 (knockout cell lysate ab257129) was used. Wild-type and Proteasome 20S LMP7 knockout samples were subjected to SDS-PAGE. ab180606 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (ab180606) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

PSMB8 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human PSMB8 (Proteasome 20S LMP7) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-psmb8-proteasome-20s-lmp7-knockout-a549-cell-line-ab267148'>ab267148</a>)

Lane 3:

Raji cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 30 kDa

Observed band size: 23 kDa

false

Western blot - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)
  • WB

Lab

Western blot - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)

Lanes 1-4 : Merged signal (red and green). Green - ab180606 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.

ab180606 Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] was shown to specifically react with Proteasome 20S LMP7 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267149 (knockout cell lysate ab257130) was used. Wild-type and Proteasome 20S LMP7 knockout samples were subjected to SDS-PAGE. ab180606 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (ab180606) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

PSMB8 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PSMB8 (Proteasome 20S LMP7) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-psmb8-proteasome-20s-lmp7-knockout-a549-cell-line-ab267149'>ab267149</a>)

Lane 3:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 30 kDa

Observed band size: 23 kDa

false

Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)

Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling Proteasome 20S LMP7 with purified ab180606 at 1/90 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Western blot - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)
  • WB

Lab

Western blot - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (AB180606)

All lanes:

Western blot - Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (ab180606) at 1/10000 dilution

All lanes:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 30 kDa

Observed band size: 23 kDa

false

  • 578 PE

    PE Anti-Proteasome 20S LMP7 antibody [EPR14482(B)]

  • Carrier free

    Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Proteasome 20S LMP7 antibody [EPR14482(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14482(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1700", "IHCP-species-notes": "<p><strong>For unpurified use at 1/250 - 1/500.</strong></p><p>Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0)</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/90 - 1/150", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome 20S LMP7 also known as β5i or PSMB8 is a subunit of the immunoproteasome. It weighs about 23 kDa and it is expressed predominantly in immune cells such as lymphocytes and macrophages. LMP7 is part of the proteasome 20S a cylindrical core particle involved in the degradation of ubiquitinated proteins. This process helps maintain protein homeostasis by breaking down misfolded and damaged proteins.
Biological function summary

The immunoproteasome in which LMP7 is an essential component plays an important role in the adaptive immune system. It influences the generation of antigenic peptides that are presented on MHC class I molecules. LMP7's expression is increased during immune responses particularly following gamma interferon (IFN-γ) stimulation contributing to the immune-mediated proteasome conformation. The immunoproteasome enables more efficient peptide processing enhancing antigen presentation to cytotoxic T lymphocytes.

Pathways

LMP7 is involved in the MHC class I antigen presentation pathway. This pathway allows the immune system to detect and respond to intracellular pathogens such as viruses. Additionally LMP7 interacts with TAP (Transporter associated with Antigen Processing) and other proteasome subunits to ensure effective peptide processing for loading onto MHC class I molecules. Through this involvement it closely links to immune signaling and cellular stress responses.

Research shows that LMP7 connects to autoimmune diseases and inflammatory disorders such as lupus and rheumatoid arthritis. Dysregulation of LMP7 expression or activity can lead to improper immune responses contributing to disease pathogenesis. Furthermore LMP7 impacts the degradation of specific proteins relevant to these conditions. For example it works alongside other proteasome subunits like LMP2 and MECL-1 in modulating the inflammatory response which can exacerbate autoimmune disease symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Involved in the generation of spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein (PubMed : 27049119). Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
See full target information PSMB8
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Cancers 17: PubMed40507366

2025

LMP7-Specific Inhibitor M3258 Modulates the Tumor Microenvironment of Triple-Negative Breast Cancer and Inflammatory Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuemei Xie,Jangsoon Lee,Ganiraju C Manyam,Troy Pearson,Gina Walter-Bausch,Manja Friese-Hamim,Sheng Zhao,Julia Jabs,Angela A Manginelli,Nadine Piske,Thomas Mrowiec,Corinna M Wolf,Bharat S Kuntal,Debu Tripathy,Jing Wang,Michael P Sanderson,Naoto T Ueno

JID innovations : skin science from molecules to population health 5:100319 PubMed39867570

2025

DNA Methyltransferase Inhibition Upregulates the Costimulatory Molecule ICAM-1 and the Immunogenic Phenotype of Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra S P Cereghetti,Patrick Turko,Phil Cheng,Stephan Benke,Ala'a Al Hrout,Andreas Dzung,Reinhard Dummer,Michael O Hottiger,Richard Chahwan,Lorenza P Ferretti,Mitchell P Levesque

Molecular cancer therapeutics 23:1743-1760 PubMed39210605

2024

Immunoproteasome Activation Expands the MHC Class I Immunopeptidome, Unmasks Neoantigens, and Enhances T-cell Anti-Myeloma Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka S Rana,James J Ignatz-Hoover,Chunna Guo,Amber L Mosley,Ehsan Malek,Yuriy Federov,Drew J Adams,James J Driscoll

Advances in rheumatology (London, England) 63:14 PubMed36949513

2023

The role of proteasome in muscle wasting of experimental arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Vivian Oliveira Nunes Teixeira,Bárbara Jonson Bartikoski,Rafaela Cavalheiro do Espirito Santo,Paulo Vinícius Gil Alabarse,Khetam Ghannan,Jordana Miranda Souza Silva,Lidiane Isabel Filippin,Fernanda Visioli,Lorena Martinez-Gamboa,Eugen Feist,Ricardo Machado Xavier

RMD open 9: PubMed36854567

2023

Immunoproteasome subunit β5i promotes perifascicular muscle atrophy in dermatomyositis by upregulating RIG-I.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Qisheng Xia,Wenli Li,Qingyan Liu,Lining Zhang,Xiaolan Tian,Lifang Ye,Guochun Wang,Qinglin Peng

Frontiers in cell and developmental biology 9:798445 PubMed35127714

2022

A Novel Prognostic Tool for Glioma Based on Enhancer RNA-Regulated Immune Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Tian,Kegong Chen,Guangcan Yan,Xinhao Han,Yanlong Liu,Qiuju Zhang,Meina Liu

Experimental and therapeutic medicine 22:1260 PubMed34603528

2021

Quercetin suppresses inflammatory cytokine production in rheumatoid arthritis fibroblast-like synoviocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Tao Sun,Jian-Peng Li,Wei-Qing Qian,Meng-Fan Yin,Hong Yin,Gui-Cheng Huang

Frontiers in immunology 11:603278 PubMed33552061

2021

Co-Inhibition of the Immunoproteasome Subunits LMP2 and LMP7 Ameliorates Immune Thrombocytopenia.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Hong Du,Yu-Jiao Xiang,Lu Liu,Mu Nie,Yu Hou,Ling Wang,Ban-Ban Li,Miao Xu,Qing-Liang Teng,Jun Peng,Ming Hou,Yan Shi

The Kaohsiung journal of medical sciences 36:494-500 PubMed32128987

2020

miR-451a is downregulated and targets PSMB8 in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Liu,Huan-Zhi Yang,Yong-Jun Jiang,Li-Qi Xu

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 20:399-410 PubMed31595669

2019

Proteasomal adaptations underlying carfilzomib-resistance in human bone marrow plasma cells.

Applications

Unspecified application

Species

Unspecified reactive species

E Steve Woodle,Simon Tremblay,Paul Brailey,Alin Girnita,Rita R Alloway,Bruce Aronow,Nupur Dasgupta,Frederic Ebstein,Peter-Michael Kloetzel,Min Jae Lee,Kyung B Kim,Harinder Singh,James J Driscoll
View all publications
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