AB125914

Anti-Proteasome 26S S2/PSMD2 antibody

Name: Anti-Proteasome 26S S2/PSMD2 antibody (ab125914) | Abcam
Brand: Abcam Limited
SKU: AB125914

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(2 Publications)

Rabbit Polyclonal Proteasome 26S S2/PSMD2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PSMD2 aa 500-750.

View Alternative Names

TRAP2, PSMD2, 26S proteasome non-ATPase regulatory subunit 2, 26S proteasome regulatory subunit RPN1, 26S proteasome regulatory subunit S2, 26S proteasome subunit p97, Protein 55.11, Tumor necrosis factor type 1 receptor-associated protein 2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S2/PSMD2 antibody (AB125914)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S2/PSMD2 antibody (AB125914)

ab125914, at 1/500, staining Proteasome 26S S2/PSMD2 antibody in Human A431 cells by Immunofluorescence (methanol-fixed).

Unknown

Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (AB125914)

Gel concentration : 5%

All lanes:

Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (ab125914) at 1/500 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 100 kDa

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Supplier Data

Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (AB125914)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (ab125914) at 1/1000 dilution

Lane 1:

JC whole cell lysates at 30 µg

Lane 2:

BCL-1 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 100 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PSMD2 aa 500-750. The exact immunogen used to generate this antibody is proprietary information.

Q13200

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome 26S S2 also known as PSMD2 is a subunit of the 26S proteasome. It plays an important role in the degradation of ubiquitinated proteins a process essential for maintaining protein homeostasis. The molecular mass of Proteasome 26S S2 is around 102 kDa. It is expressed in various tissues including the brain heart and muscle. The protein facilitates the recognition and unfolding of substrates destined for proteolysis by the 26S proteasome an activity that is instrumental in controlling the quality and abundance of proteins within the cell.

Biological function summary

Proteasome 26S S2 is an integral part of the 26S proteasome complex a large protease complex with a 20S core particle and 19S regulatory particles. This complex is responsible for degrading most intracellular proteins and is vital for regulating numerous cellular processes such as cell cycle control DNA repair and signal transduction. PSMD2 is linked with the assembly and stabilization of the 19S regulatory particle influencing the efficiency of the proteasome in recognizing and processing polyubiquitinated proteins.

Pathways

Proteasome 26S S2 is involved in ubiquitin-proteasome pathway and cell cycle regulation. In the ubiquitin-proteasome pathway it works with ubiquitin ligases and deubiquitinating enzymes to ensure selective and timely protein turnover. It is also associated with important regulatory proteins like CDC48 VCP/P97 which are involved in the ATP-dependent unfolding of proteins before proteasomal degradation. These pathways are essential for maintaining cellular protein homeostasis and managing cell cycle transitions suggesting an important regulatory role played by Proteasome 26S S2.

Proteasome 26S S2 is linked to neurodegenerative diseases and cancers. In neurodegenerative diseases such as Parkinson's disease dysfunction of the proteasome can lead to the accumulation of misfolded proteins thereby exacerbating disease progression. In cancers overexpression of Proteasome 26S S2 and other proteasomal subunits could promote the degradation of tumor suppressor proteins fueling carcinogenesis. It connects with proteins like PINK1 and PARKIN in neurodegeneration and interacts with p53 in cancer pathways indicating its critical involvement in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.. Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.

See full target information PSMD2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3427 PubMed37296096

2023

Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan P Hildebrandt,Kathryn R Moss,Aleksandra Janusz-Kaminska,Luke A Knudson,Lance T Denes,Tanvi Saxena,Devi Prasad Boggupalli,Zhuangyue Li,Kun Lin,Gary J Bassell,Eric T Wang

Nature communications 12:3481 PubMed34108482

2021

TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Ou,Jia-Qi Fang,Zhe-Sheng Zhang,Zhe Chi,Jie Fang,Di-Yan Xu,Kai-Zhong Lu,Meng-Qing Qian,Da-Yong Zhang,Jun-Ping Guo,Wei Gao,Na-Ru Zhang,Jian-Ping Pan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome 26S S2/PSMD2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S2/PSMD2 antibody (AB125914)

Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (AB125914)

Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (AB125914)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise