Anti-Proteasome 26S S2/PSMD2 antibody
5
(2 Reviews)
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(2 Publications)
Rabbit Polyclonal Proteasome 26S S2/PSMD2 antibody. Suitable for IP, WB, IHC-Fr and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PSMD2 aa 50-100.
View Alternative Names
TRAP2, PSMD2, 26S proteasome non-ATPase regulatory subunit 2, 26S proteasome regulatory subunit RPN1, 26S proteasome regulatory subunit S2, 26S proteasome subunit p97, Protein 55.11, Tumor necrosis factor type 1 receptor-associated protein 2
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Proteasome 26S S2/PSMD2 antibody (AB140675)
Immunohistochemical analysis of frozen section of human ovarian carcinoma with ab140675 at 1/1000 (1 μg/ml) dilution.
Detection : DAB
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Proteasome 26S S2/PSMD2 antibody (AB140675)
Immunohistochemical analysis of frozen section of mouse renal cell carcinoma with ab140675 at 1/1000 (1 μg/ml) dilution.
Detection : DAB
- WB
Unknown
Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (AB140675)
All lanes:
Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (ab140675) at 0.4 µg/mL
Lane 1:
Whole cell lysate prepared from 293T cells at 50 µg
Lane 2:
Whole cell lysate prepared from 293T cells at 15 µg
Lane 3:
Whole cell lysate prepared from HeLa cells at 50 µg
Lane 4:
Whole cell lysate prepared from Jurkat cells at 50 µg
Predicted band size: 100 kDa
true
Exposure time: 30s
- IP
Unknown
Immunoprecipitation - Anti-Proteasome 26S S2/PSMD2 antibody (AB140675)
Detection of Human Proteasome 26S S2/PSMD2 by Western Blot of Immunoprecipitates.Samples : Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells.
Lane 1 : IP with an antibody which recognizes a different epitope of anti-Proteasome 26S S2/PSMD2 antibody.
Lane 2 : ab140675 used for IP at 6 μg/mg lysate.
Lane 3 : Control IgG.For blotting immunoprecipitated Proteasome 26S S2/PSMD2, ab140675 was used at 1 μg/ml.Detection : Chemiluminescence with an exposure time of 3 seconds.
All lanes:
Immunoprecipitation - Anti-Proteasome 26S S2/PSMD2 antibody (ab140675)
Predicted band size: 100 kDa
false
- WB
Unknown
Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (AB140675)
All lanes:
Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (ab140675) at 0.4 µg/mL
All lanes:
whole cell lysate prepared from NIH3T3 cells at 50 µg
Predicted band size: 100 kDa
true
Exposure time: 3min
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Proteasome 26S S2 is an integral part of the 26S proteasome complex a large protease complex with a 20S core particle and 19S regulatory particles. This complex is responsible for degrading most intracellular proteins and is vital for regulating numerous cellular processes such as cell cycle control DNA repair and signal transduction. PSMD2 is linked with the assembly and stabilization of the 19S regulatory particle influencing the efficiency of the proteasome in recognizing and processing polyubiquitinated proteins.
Pathways
Proteasome 26S S2 is involved in ubiquitin-proteasome pathway and cell cycle regulation. In the ubiquitin-proteasome pathway it works with ubiquitin ligases and deubiquitinating enzymes to ensure selective and timely protein turnover. It is also associated with important regulatory proteins like CDC48 VCP/P97 which are involved in the ATP-dependent unfolding of proteins before proteasomal degradation. These pathways are essential for maintaining cellular protein homeostasis and managing cell cycle transitions suggesting an important regulatory role played by Proteasome 26S S2.
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Target data
Publications (2)
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Cell death & disease 14:685 PubMed37848434
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cancer letters 430:109-122 PubMed29777785
2018
Applications
Unspecified application
Species
Unspecified reactive species
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