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AB154963

Anti-Proteasome 26S S3/PSMD3 antibody

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Rabbit Polyclonal Proteasome 26S S3/PSMD3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PSMD3 aa 1-250.

View Alternative Names

26S proteasome non-ATPase regulatory subunit 3, 26S proteasome regulatory subunit RPN3, 26S proteasome regulatory subunit S3, Proteasome subunit p58, PSMD3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 26S S3/PSMD3 antibody (AB154963)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 26S S3/PSMD3 antibody (AB154963)

Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling Proteasome 26S S3/PSMD3 with ab154963 at 1/500.

Western blot - Anti-Proteasome 26S S3/PSMD3 antibody (AB154963)
  • WB

Unknown

Western blot - Anti-Proteasome 26S S3/PSMD3 antibody (AB154963)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Proteasome 26S S3/PSMD3 antibody (ab154963) at 1/500 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 61 kDa

false

Western blot - Anti-Proteasome 26S S3/PSMD3 antibody (AB154963)
  • WB

Unknown

Western blot - Anti-Proteasome 26S S3/PSMD3 antibody (AB154963)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Proteasome 26S S3/PSMD3 antibody (ab154963) at 1/1000 dilution

All lanes:

NIH 3T3 whole cell lysate at 30 µg/mL

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PSMD3 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O43242

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Proteasome 26S S3 also known as PSMD3 is an essential component of the 26S proteasome complex which plays a critical role in degrading ubiquitinated proteins. It has a molecular mass of approximately 61 kDa and is important in maintaining protein homeostasis by regulating protein degradation. The 26S proteasome is expressed ubiquitously in eukaryotic cells indicating its importance in various cellular processes. Its activity ensures the removal of damaged or misfolded proteins which is necessary for proper cellular function and survival.
Biological function summary

Proteasome 26S S3 forms an integral part of the 26S proteasome a large complex involved in the ATP-dependent breakdown of proteins. This complex is essential for many cellular functions including cell cycle progression signal transduction and stress responses. By facilitating protein degradation Proteasome 26S S3 helps regulate the quantity and quality of proteins within the cell. It interacts with other proteasomal components to mediate the recognition and unfolding of target proteins enabling their subsequent degradation into smaller peptides.

Pathways

The Proteasome 26S S3 participates in the ubiquitin-proteasome pathway and the NF-κB signaling pathway. These pathways are pivotal in controlling protein turnover and are involved in cellular responses to various stimuli. Within the ubiquitin-proteasome pathway PSMD3 collaborates with other proteasome components to execute protein degradation processes. In the NF-κB pathway PSMD3 influences the degradation of IκB proteins allowing the activation of NF-κB transcription factors which regulate immune and inflammatory responses.

Dysregulation of Proteasome 26S S3 activity has links to cancer and neurodegenerative diseases. Abnormal proteasome function can result in the accumulation of damaged proteins contributing to the pathogenesis of these conditions. In cancer alterations in the proteasome function can lead to uncontrolled cell growth and survival where PSMD3 interacts with proteins like p53 and Bcl-2 affecting apoptosis and cell cycle regulation. In neurodegenerative diseases compromised proteasome activity may lead to the accumulation of misfolded proteins exacerbating conditions such as Alzheimer's or Parkinson's disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.
See full target information PSMD3
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Product promise

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