Anti-Proteasome 26S S3/PSMD3 antibody
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(1 Publication)
Rabbit Polyclonal Proteasome 26S S3/PSMD3 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PSMD3 aa 500 to C-terminus.
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26S proteasome non-ATPase regulatory subunit 3, 26S proteasome regulatory subunit RPN3, 26S proteasome regulatory subunit S3, Proteasome subunit p58, PSMD3
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (AB3316)
Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (AB3316)
Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-Proteasome 26S S3/PSMD3 antibody (AB3316)
Flow Cytometry analysis of HeLa cells labelng Proteasome 26S S3/PSMD3 with ab3316 (Pink) or a rabbit IgG isotype control (Black) 10 μg/mL. Goat anti-Rabbit IgG (H+L) Superclonal™ Alexa Fluor® 647 conjugate at a dilution of 1/50 was used as the Secondary Antibody.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (AB3316)
Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Reactivity data
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Supplementary information
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Biological function summary
Proteasome 26S S3 forms an integral part of the 26S proteasome a large complex involved in the ATP-dependent breakdown of proteins. This complex is essential for many cellular functions including cell cycle progression signal transduction and stress responses. By facilitating protein degradation Proteasome 26S S3 helps regulate the quantity and quality of proteins within the cell. It interacts with other proteasomal components to mediate the recognition and unfolding of target proteins enabling their subsequent degradation into smaller peptides.
Pathways
The Proteasome 26S S3 participates in the ubiquitin-proteasome pathway and the NF-κB signaling pathway. These pathways are pivotal in controlling protein turnover and are involved in cellular responses to various stimuli. Within the ubiquitin-proteasome pathway PSMD3 collaborates with other proteasome components to execute protein degradation processes. In the NF-κB pathway PSMD3 influences the degradation of IκB proteins allowing the activation of NF-κB transcription factors which regulate immune and inflammatory responses.
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Target data
Publications (1)
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Nature materials 16:953-961 PubMed28783156
2017
Applications
Unspecified application
Species
Unspecified reactive species
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