Rabbit Polyclonal Proteasome Activator Subunit 4/PSME4 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PSME4 aa 1-50.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PSME4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q14997
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.
Alternative names
BLM10, KIAA0077, PSME4, Proteasome activator complex subunit 4, Proteasome activator PA200, Protein BLM10 homolog, Blm10, hBlm10
Recommended products
Rabbit Polyclonal Proteasome Activator Subunit 4/PSME4 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PSME4 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PSME4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q14997
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab157158 was affinity purified using an epitope specific to Proteasome Activator Subunit 4/PSME4 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Proteasome Activator Subunit 4 (PSME4) also known as PA200 is an important component in regulating protein degradation. It belongs to the family of proteasome activator subunits and has a molecular mass of approximately 230 kDa. PSME4 is mainly expressed in the nucleus of cells and modulates proteasome activity by binding to the proteasome core particle. Through facilitating protein breakdown PSME4 significantly influences various cellular processes including the elimination of oxidatively damaged proteins.
- Biological function summary
PSME4 functions as part of a complex that enhances proteasome-mediated protein degradation. This complex formation improves the efficiency of protein processing by recruiting specific substrates for degradation. PSME4 is involved in DNA repair processes and plays a role in spermatogenesis contributing to proper cell cycle progression and genomic stability. The protein's activity in these biological functions demonstrates its importance in maintaining cellular homeostasis.
- Pathways
PSME4 is intricately involved in the ubiquitin-proteasome pathway and the DNA damage response pathway which are essential for maintaining protein quality control and genomic integrity. PSME4 interacts with other proteins such as RAD23B facilitating the repair of damaged DNA. By integrating into these pathways PSME4 ensures the proper execution of critical cellular processes and responds to stress-related signals.
- Associated diseases and disorders
Alterations in PSME4 expression or function have connections to cancer progression and neurodegenerative diseases. In cancer PSME4 may influence tumor growth and resistance to therapy through its role in DNA repair and protein turnover. Additionally in neurodegenerative conditions like Alzheimer's disease PSME4-associated proteasome dysfunction contributes to the accumulation of toxic protein aggregates. Understanding PSME4 interactions with proteins like p53 in cancer or tau in neurodegeneration offers insights into potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158)
All lanes: Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158) at 0.4 µg/mL
Lane 1: 293T wholecell lysate at 50 µg
Lane 2: 293T wholecell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 50 µg
Lane 4: Jurkat wholecell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 211 kDa
Exposure time: 3min
Immunoprecipitation - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158)
Detection of Proteasome Activator Subunit 4/PSME4 by Western Blot of Immunprecipitate.
ab157158 at 1μg/ml labeling Proteasome Activator Subunit 4/PSME4 in 293T whole cell lysate immunoprecipitated using ab157158 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.All lanes: Immunoprecipitation - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158)
Predicted band size: 211 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158)
Immunohistochemistry if human non-small cell lung cancer staining Proteasome Activator Subunit 4/PSME4 with ab157158 at 1/1000.
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