AB170865

Anti-Proteasome maturation protein antibody [EPR10177]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Proteasome maturation protein antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

C13orf12, UMP1, HSPC014, HSPC036, PNAS-110, POMP, Proteasome maturation protein, Proteassemblin, Protein UMP1 homolog, Voltage-gated K channel beta subunit 4.1, hUMP1

4 Images

Flow Cytometry (Intracellular) - Anti-Proteasome maturation protein antibody [EPR10177] (AB170865)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Proteasome maturation protein antibody [EPR10177] (AB170865)

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling proteasome maturation protein with ab170865 at 1/100 dilution (red)or a rabbit IgG (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome maturation protein antibody [EPR10177] (AB170865)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome maturation protein antibody [EPR10177] (AB170865)

Immunohistochemical analysis of Formalin-fixedparaffin-embedded Human skin tissue labeling proteasome maturation protein with ab170865 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Proteasome maturation protein antibody [EPR10177] (AB170865)

Detection of proteasome maturation protein by Western Blot of Immunprecipitate.
HeLa cell lysate immunoprecipitated using ab170865 at 1/10 dilution (Lane 1) or 1X PBS (negative control) (Lane 2) and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)

Predicted band size: 16 kDa

false

Supplier Data

Western blot - Anti-Proteasome maturation protein antibody [EPR10177] (AB170865)

All lanes:

Western blot - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SW480 cell lysate at 10 µg

Lane 3:

HL-60 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 16 kDa

false

Carrier free

Anti-Proteasome maturation protein antibody [EPR10177] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10177

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The proteasome maturation protein also known as POMP or proteassemblin plays an important role in proteasome biogenesis. It is an important factor involved in the assembly of 20S proteasomes serving as a chaperone that regulates the efficient formation of this complex. The mass of the proteasome maturation protein is approximately 21 kDa. It is expressed in various tissues including muscle liver and skin indicating its broad significance in human physiology.

Biological function summary

Proteasome maturation protein assists in the assembly and maturation of the 20S core particle which is central to the proteasome's function. This protein is a critical component of the proteasome assembly chaperone (PAC) complex which includes other chaperones that work together to construct the 20S proteasome. The activity of POMP is fundamental for maintaining cellular homeostasis by managing protein degradation.

Pathways

Proteasome maturation protein plays an important role in the ubiquitin-proteasome pathway a major protein degradation pathway. This process involves tagging defective or unneeded proteins with ubiquitin which are then degraded by the proteasome. The ubiquitin-proteasome pathway coordinates with other pathways such as the NF-kB signaling pathway where it regulates the degradation of inhibitors like IκB facilitating NF-kB activation. By doing so POMP through the proteasome indirectly influences various physiological processes controlled by these pathways.

Alterations in the function of proteasome maturation protein have connections with conditions like cancer and autoimmune diseases. For instance abnormal proteasome activity linked to POMP can contribute to the development of multiple myeloma where proteasome inhibitors are used therapeutically to control protein degradation. Additionally autoimmunity involves irregularities in proteasome functions affecting the proteins processed by the immune system such as the MHC class I molecules. Understanding how POMP contributes to these illnesses can influence treatment strategies leveraging its central role in proteasome assembly and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum.

See full target information POMP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 32:108154 PubMed32937139

2020

Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Kim Schneider,Geoffrey Michael Nelson,Joseph Luke Watson,Jörg Morf,Maximillian Dalglish,Laura Martina Luh,Annika Weber,Anne Bertolotti

Nature 536:184-9 PubMed27462806

2016

An evolutionarily conserved pathway controls proteasome homeostasis.

Applications

Species

Unspecified reactive species

Adrien Rousseau,Anne Bertolotti

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome maturation protein antibody [EPR10177]

Flow Cytometry (Intracellular) - Anti-Proteasome maturation protein antibody [EPR10177] (AB170865)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome maturation protein antibody [EPR10177] (AB170865)

Immunoprecipitation - Anti-Proteasome maturation protein antibody [EPR10177] (AB170865)

Western blot - Anti-Proteasome maturation protein antibody [EPR10177] (AB170865)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise