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AB249548

Anti-Proteasome maturation protein antibody [EPR10177] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Proteasome maturation protein antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C13orf12, UMP1, HSPC014, HSPC036, PNAS-110, POMP, Proteasome maturation protein, Proteassemblin, Protein UMP1 homolog, Voltage-gated K channel beta subunit 4.1, hUMP1

4 Images
Flow Cytometry (Intracellular) - Anti-Proteasome maturation protein antibody [EPR10177] - BSA and Azide free (AB249548)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Proteasome maturation protein antibody [EPR10177] - BSA and Azide free (AB249548)

This data was developed using ab170865, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling proteasome maturation protein with ab170865 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome maturation protein antibody [EPR10177] - BSA and Azide free (AB249548)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome maturation protein antibody [EPR10177] - BSA and Azide free (AB249548)

This data was developed using ab170865, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Formalin-fixed,paraffin-embedded Human skin tissue labeling proteasome maturation protein with ab170865 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Proteasome maturation protein antibody [EPR10177] - BSA and Azide free (AB249548)
  • IP

Supplier Data

Immunoprecipitation - Anti-Proteasome maturation protein antibody [EPR10177] - BSA and Azide free (AB249548)

This data was developed using ab170865, the same antibody clone in a different buffer formulation.

Detection of proteasome maturation protein by Western Blot of Immunprecipitate.
HeLa cell lysate immunoprecipitated using ab170865 at 1/10 dilution (Lane 1) or 1X PBS (negative control) (Lane 2) and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Proteasome maturation protein antibody [EPR10177] (<a href='/en-us/products/primary-antibodies/proteasome-maturation-protein-antibody-epr10177-ab170865'>ab170865</a>)

Predicted band size: 16 kDa

false

Western blot - Anti-Proteasome maturation protein antibody [EPR10177] - BSA and Azide free (AB249548)
  • WB

Supplier Data

Western blot - Anti-Proteasome maturation protein antibody [EPR10177] - BSA and Azide free (AB249548)

This data was developed using ab170865, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Proteasome maturation protein antibody [EPR10177] (<a href='/en-us/products/primary-antibodies/proteasome-maturation-protein-antibody-epr10177-ab170865'>ab170865</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SW480 cell lysate at 10 µg

Lane 3:

HL-60 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 16 kDa

false

  • Unconjugated

    Anti-Proteasome maturation protein antibody [EPR10177]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10177

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab249548 is the carrier-free version of ab170865.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The proteasome maturation protein also known as POMP or proteassemblin plays an important role in proteasome biogenesis. It is an important factor involved in the assembly of 20S proteasomes serving as a chaperone that regulates the efficient formation of this complex. The mass of the proteasome maturation protein is approximately 21 kDa. It is expressed in various tissues including muscle liver and skin indicating its broad significance in human physiology.
Biological function summary

Proteasome maturation protein assists in the assembly and maturation of the 20S core particle which is central to the proteasome's function. This protein is a critical component of the proteasome assembly chaperone (PAC) complex which includes other chaperones that work together to construct the 20S proteasome. The activity of POMP is fundamental for maintaining cellular homeostasis by managing protein degradation.

Pathways

Proteasome maturation protein plays an important role in the ubiquitin-proteasome pathway a major protein degradation pathway. This process involves tagging defective or unneeded proteins with ubiquitin which are then degraded by the proteasome. The ubiquitin-proteasome pathway coordinates with other pathways such as the NF-kB signaling pathway where it regulates the degradation of inhibitors like IκB facilitating NF-kB activation. By doing so POMP through the proteasome indirectly influences various physiological processes controlled by these pathways.

Alterations in the function of proteasome maturation protein have connections with conditions like cancer and autoimmune diseases. For instance abnormal proteasome activity linked to POMP can contribute to the development of multiple myeloma where proteasome inhibitors are used therapeutically to control protein degradation. Additionally autoimmunity involves irregularities in proteasome functions affecting the proteins processed by the immune system such as the MHC class I molecules. Understanding how POMP contributes to these illnesses can influence treatment strategies leveraging its central role in proteasome assembly and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum.
See full target information POMP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 7:78242-78254 PubMed27793041

2016

CPI-17 drives oncogenic Ras signaling in human melanomas via Ezrin-Radixin-Moesin family proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Lars Björn Riecken,Ansgar Zoch,Ulrike Wiehl,Sabine Reichert,Ingmar Scholl,Yan Cui,Mirjana Ziemer,Ulf Anderegg,Christian Hagel,Helen Morrison
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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