AB140426

Anti-Proteasome subunit beta type 2/PSMB2 antibody

Name: Anti-Proteasome subunit beta type 2/PSMB2 antibody (ab140426) | Abcam
Brand: Abcam Limited
SKU: AB140426
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Proteasome subunit beta type 2/PSMB2 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PSMB2 aa 150 to C-terminus.

View Alternative Names

Proteasome subunit beta type-2, Macropain subunit C7-I, Multicatalytic endopeptidase complex subunit C7-I, Proteasome component C7-I, Proteasome subunit beta-4, beta-4, PSMB2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB140426)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB140426)

Immunohistochemistry of human ovarian carcinoma tissue using ab140426 at 1/5000.

Unknown

Immunoprecipitation - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB140426)

Detection of Proteasome subunit beta type 2/PSMB2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab140426 at 6 μg/mg lysate for IP (Lane 1). Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Proteasome subunit beta type 2/PSMB2 antibody (ab140426)

Predicted band size: 23 kDa

false

Unknown

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB140426)

All lanes:

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody (ab140426) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 23 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human PSMB2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P49721

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab140426 was affinity purified using an epitope specific to Proteasome subunit beta type 2/PSMB2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome subunit beta type 2 also known as PSMB2 is a part of the proteasome complex which plays an important role in protein degradation. PSMB2 has a mass of about 22 kDa and functions within the multi-catalytic proteinase complex known as the 20S proteasome core particle. This protein is expressed in various tissues with significant activities found in cells that demand high protein turnover. PSMB2 contributes to the proteasome's chymotrypsin-like activity aiding in the breakdown of damaged or unneeded proteins into peptides.

Biological function summary

Proteasome subunit beta type 2 is essential in maintaining protein homeostasis. It functions as a component of the 20S core of the 26S proteasome where it participates in degrading ubiquitin-tagged proteins. This complex also regulates multiple cellular processes including cell cycle progression and signal transduction. Proper function of PSMB2 is necessary for removing misfolded or damaged proteins thereby preventing their accumulation which could be toxic to cells.

Pathways

Proteasome subunit beta type 2 has a role in the ubiquitin-proteasome pathway which is important for protein degradation and turnover. This pathway contributes to important cellular processes such as DNA repair and antigen processing. PSMB2 in this context is linked to other proteins like ubiquitin-ligase that mediate the tagging of proteins for degradation. Another pathway involving PSMB2 is the NF-kB signaling pathway which controls inflammatory responses and immune system regulation.

Proteasome subunit beta type 2 has been implicated in neurodegenerative diseases like Parkinson’s. The dysregulation or malfunctioning of the proteasome complex might result in protein aggregation seen in such disorders. Additionally PSMB2 is associated with certain cancers due to its role in regulating apoptosis and cellular proliferative activities. Proteins like p53 which is important for tumor suppression are related to PSMB2 through these disease mechanisms highlighting its significance in maintaining normal cellular function and preventing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

See full target information PSMB2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Pharmacology 108:47-60 PubMed36423586

2022

Luteolin Attenuates Diabetic Myocardial Hypertrophy by Inhibiting Proteasome Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Bing Li,Mubarak Rekep,Jia-Hui Tian,Qian Wu,Mei Chen,Shuo Yang,Lu-Xuan Zhang,Gui-Ping Zhang,Yuan Qin,Xi-Yong Yu,Qin Xue,Ying-Hua Liu

Aging 13:364-375 PubMed33290263

2020

Enhancer-bound Nrf2 licenses HIF-1α transcription under hypoxia to promote cisplatin resistance in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Jin,Liansheng Gong,Ying Peng,Le Li,Gang Liu

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 20:399-410 PubMed31595669

2019

Proteasomal adaptations underlying carfilzomib-resistance in human bone marrow plasma cells.

Applications

Unspecified application

Species

Unspecified reactive species

E Steve Woodle,Simon Tremblay,Paul Brailey,Alin Girnita,Rita R Alloway,Bruce Aronow,Nupur Dasgupta,Frederic Ebstein,Peter-Michael Kloetzel,Min Jae Lee,Kyung B Kim,Harinder Singh,James J Driscoll

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Proteasome subunit beta type 2/PSMB2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB140426)

Immunoprecipitation - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB140426)

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB140426)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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