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AB236752

Anti-Proteasome subunit beta type 2/PSMB2 antibody

1

(1 Review)

|

(3 Publications)

Rabbit Polyclonal Proteasome subunit beta type 2/PSMB2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PSMB2.

View Alternative Names

Proteasome subunit beta type-2, Macropain subunit C7-I, Multicatalytic endopeptidase complex subunit C7-I, Proteasome component C7-I, Proteasome subunit beta-4, beta-4, PSMB2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB236752)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB236752)

PC-3 (human prostate adenocarcinoma cell line) cells stained for Proteasome subunit beta type 2/PSMB2 (green) using ab236752 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB236752)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB236752)

Paraffin-embedded mouse kidney tissue stained for Proteasome subunit beta type 2/PSMB2 using ab236752 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB236752)
  • WB

Supplier Data

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody (AB236752)

All lanes:

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody (ab236752) at 1/500 dilution

Lane 1:

EC109 whole cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/15000 dilution

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PSMB2.

P49721

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Complementary Products

Assay Kits

AB107921

Proteasome Activity Assay Kit

Functional Studies - Proteasome Activity Assay Kit (AB107921)

  • 4
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB22673

Anti-Proteasome 20S alpha + beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha + beta antibody (AB22673)

  • 5
  • 7
Primary Antibodies

AB247896

Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] - BSA and Azide free (AB247896)

  • 0
  • 0
Primary Antibodies

AB181105

Anti-USP4 antibody [EPR13846]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody [EPR13846] (AB181105)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome subunit beta type 2 also known as PSMB2 is a part of the proteasome complex which plays an important role in protein degradation. PSMB2 has a mass of about 22 kDa and functions within the multi-catalytic proteinase complex known as the 20S proteasome core particle. This protein is expressed in various tissues with significant activities found in cells that demand high protein turnover. PSMB2 contributes to the proteasome's chymotrypsin-like activity aiding in the breakdown of damaged or unneeded proteins into peptides.
Biological function summary

Proteasome subunit beta type 2 is essential in maintaining protein homeostasis. It functions as a component of the 20S core of the 26S proteasome where it participates in degrading ubiquitin-tagged proteins. This complex also regulates multiple cellular processes including cell cycle progression and signal transduction. Proper function of PSMB2 is necessary for removing misfolded or damaged proteins thereby preventing their accumulation which could be toxic to cells.

Pathways

Proteasome subunit beta type 2 has a role in the ubiquitin-proteasome pathway which is important for protein degradation and turnover. This pathway contributes to important cellular processes such as DNA repair and antigen processing. PSMB2 in this context is linked to other proteins like ubiquitin-ligase that mediate the tagging of proteins for degradation. Another pathway involving PSMB2 is the NF-kB signaling pathway which controls inflammatory responses and immune system regulation.

Proteasome subunit beta type 2 has been implicated in neurodegenerative diseases like Parkinson's. The dysregulation or malfunctioning of the proteasome complex might result in protein aggregation seen in such disorders. Additionally PSMB2 is associated with certain cancers due to its role in regulating apoptosis and cellular proliferative activities. Proteins like p53 which is important for tumor suppression are related to PSMB2 through these disease mechanisms highlighting its significance in maintaining normal cellular function and preventing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
See full target information PSMB2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 222: PubMed40644312

2025

The STING pathway drives noninflammatory neurodegeneration in NGLY1 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Yang,Gustavo Torres-Ramirez,Nicole Dobbs,Jie Han,Makoto Asahina,Reiko Fujinawa,Kun Song,Yun Liu,Weichun Lin,Angelica Oviedo,Chuo Chen,Lei Zhu,William F Mueller,Kevin Lee,Tadashi Suzuki,Nan Yan

Journal of experimental & clinical cancer research : CR 43:68 PubMed38439082

2024

MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobin Wang,Yingqing Shi,Hua Shi,Xiaoyu Liu,Aijun Liao,Zhuogang Liu,Robert Z Orlowski,Rui Zhang,Huihan Wang

Cytotechnology 74:491-502 PubMed36110152

2022

PSMB2 knockdown suppressed proteasome activity and cell proliferation, promoted apoptosis, and blocked NRF1 activation in gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zimeng Liu,Changda Yu,Zhibing Chen,Chuanwen Zhao,Lin Ye,Chen Li
View all publications
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Product promise

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