Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (AB22650)

IHC image of ab22650 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22650, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• Flow Cyt

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Flow Cytometry - Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (AB22650)

Overlay histogram showing HeLa cells stained with ab22650 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22650, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

• WB

Supplier Data

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (AB22650)

All lanes:

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (ab22650) at 1/1000 dilution

All lanes:

HeLa cell lysate

Predicted band size: 23 kDa

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• WB

AbReview7252****

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (AB22650)

Lane 1:

Protein Marker IV

Lanes 2 - 7:

Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (ab22650) at 1/1000 dilution

Lane 1:

Protein Marker IV

Lanes 2, 4 and 6:

Protein extract from ciliary body of diseased human donor at 2 µg

Lanes 3, 5 and 7:

Protein extract from ciliary body of normal human donor at 2 µg

Secondary

All lanes:

HRP conjugated goat anti mouse antibody

Predicted band size: 23 kDa

Observed band size: 27 kDa

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This image is courtesy of an Abreview submitted by Dr Matthias Zenkel