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AB154745

Anti-Proteasome subunit beta type-7 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal Proteasome 20S beta 7/PSMB7 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PSMB7 aa 50 to C-terminus.

View Alternative Names

Z, PSMB7, Proteasome subunit beta type-7, Macropain chain Z, Multicatalytic endopeptidase complex chain Z, Proteasome subunit Z, Proteasome subunit beta-2, beta-2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome subunit beta type-7 antibody (AB154745)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome subunit beta type-7 antibody (AB154745)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Proteasome subunit beta type-7 with ab154745 at 1/200 dilution. Lower panel was co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type-7 antibody (AB154745)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type-7 antibody (AB154745)

Immunohistochemical analysis of paraffin-embedded MDA-MB-157 xenograft labeling Proteasome subunit beta type-7 with ab154745 at 1/100 dilution.

Western blot - Anti-Proteasome subunit beta type-7 antibody (AB154745)
  • WB

Unknown

Western blot - Anti-Proteasome subunit beta type-7 antibody (AB154745)

12% SDS PAGE

All lanes:

Western blot - Anti-Proteasome subunit beta type-7 antibody (ab154745) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 19 kDa,30 kDa

false

Western blot - Anti-Proteasome subunit beta type-7 antibody (AB154745)
  • WB

Unknown

Western blot - Anti-Proteasome subunit beta type-7 antibody (AB154745)

12% SDS PAGE

All lanes:

Western blot - Anti-Proteasome subunit beta type-7 antibody (ab154745) at 1/1000 dilution

All lanes:

mouse brain whole cell lysate at 50 µg

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PSMB7 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99436

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome subunit beta type-7 also known as PSB7 or PSMB7 is an essential component of the 20S core proteasome complex. It has a molecular weight of approximately 27 kDa. This protein is expressed ubiquitously in human tissues indicating its broad role in cellular functions. As a catalytic subunit PSB7 contributes to the proteolytic core of the proteasome which is responsible for degrading ubiquitinated proteins through ATP-dependent proteolysis.
Biological function summary

PSB7 forms part of the larger 26S proteasome complex that plays a significant role in maintaining cellular protein homeostasis. The complex is vital for degrading misfolded damaged or short-lived regulatory proteins. The degradation process by the proteasome prevents the accumulation of defective proteins which can be harmful. PSB7 along with other beta subunits contributes to the protease activity within the central chamber of the proteasome ensuring proper peptide cleavage.

Pathways

PSB7's involvement is key to the ubiquitin-proteasome pathway an essential cellular process for protein turnover. This pathway regulates various cellular processes including cell cycle apoptosis and responses to oxidative stress. PSB7 through the proteasome interacts with proteins like ubiquitin ligases and deubiquitinating enzymes which attach or remove ubiquitin tags from substrate proteins. Additionally PSB7 is linked to the NF-kB signaling pathway where it participates in the degradation of inhibitors that regulate NF-kB activity.

PSB7 plays a significant role in multiple myeloma and various types of cancer. Dysregulation of the proteasome activity with changed expression or function of PSB7 can lead to uncontrolled cell proliferation and tumor development. It also finds relevance in neurodegenerative diseases such as Alzheimer's disease where proteasome dysfunction can cause improper protein clearance and aggregation. In these diseases proteins like tau in Alzheimer's are substrates for the proteasome degradation pathway involving PSB7.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB7 displays a trypsin-like activity.
See full target information PSMB7
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2419607122 PubMed39964708

2025

Tracking proteasome degradation: A cross-organ analysis via intact degradomics mass spectrometry.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina I Zittlau,Daniel Zachor-Movshovitz,Yegor Leushkin,Roy Schimmel Brener,David Morgenstern,Gili Ben-Nissan,Michal Sharon

Life science alliance 2: PubMed31431473

2019

A molecule inducing androgen receptor degradation and selectively targeting prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Serge Auvin,Harun Öztürk,Yusuf T Abaci,Gisele Mautino,Florence Meyer-Losic,Florence Jollivet,Tarig Bashir,Hugues de Thé,Umut Sahin
View all publications
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Product promise

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