AB217857

Anti-Protective protein/Cathepsin A (PPCA) antibody

Name: Anti-Protective protein/Cathepsin A (PPCA) antibody (ab217857) | Abcam
Brand: Abcam Limited
SKU: AB217857
Price: 510 USD
Availability: InStock

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal Protective protein/Cathepsin A (PPCA) antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CTSA aa 400-500 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PPGB, CTSA, Lysosomal protective protein, Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, Protective protein cathepsin A, Protective protein for beta-galactosidase, PPCA

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protective protein/Cathepsin A (PPCA) antibody (AB217857)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protective protein/Cathepsin A (PPCA) antibody (AB217857)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat liver tissue labeling Protective protein/Cathepsin A (PPCA) with ab217857 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-Protective protein/Cathepsin A (PPCA) antibody (AB217857)

All lanes:

Western blot - Anti-Protective protein/Cathepsin A (PPCA) antibody (ab217857) at 1/300 dilution

All lanes:

293T lysates

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CTSA aa 400-500 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P10619

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protective protein/Cathepsin A (PPCA) also known as cathepsin A or protective proteins is an enzyme with a molecular mass of approximately 54 kDa. The enzyme is expressed widely in different tissues including liver kidney and the central nervous system. Mechanically PPCA plays a role as a carboxypeptidase with serine-type protease activity important for the stabilization and activation of lysosomal enzymes such as beta-galactosidase and neuraminidase. This stabilization is vital for proper lysosomal function and the breakdown of glycoproteins within the cell.

Biological function summary

In conjunction with these enzymes PPCA forms part of the lysosomal multienzyme complex. It regulates the degradation of sialylated glycoconjugates by ensuring the structural integrity and enzymatic activity of lysosomal enzymes. This regulation prevents the accumulation of substrate materials which can disrupt cellular homeostasis. PPCA's interaction with beta-galactosidase and neuraminidase enables the proper catabolism of glycoproteins and glycolipids which is essential for cellular functions.

Pathways

PPCA functions within the lysosomal storage and sialic acid catabolism pathways. These pathways are significant for the degradation and recycling of macromolecules in the lysosome maintaining cellular health. In the sialic acid metabolism pathway PPCA pairs with neuraminidase to break down sialylated substrates while interacting closely with beta-galactosidase in glycoprotein catabolism. These interactions highlight its role in preserving the efficiency of lysosomal degradation processes.

Mutations in the PPCA gene can lead to diseases like galactosialidosis and neuraminidase deficiency. Galactosialidosis arises from defective cathepsin A activity disrupting normal glycoprotein breakdown and resulting in storage disorders. This condition involves both beta-galactosidase and neuraminidase which accumulate as a result of PPCA dysfunction. Similarly neuraminidase deficiency exhibits symptoms due to impaired degradation of sialic acids indicating the necessity of PPCA in disease prevention.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.

See full target information CTSA

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 297:101337 PubMed34688655

2021

The sialidase NEU1 directly interacts with the juxtamembranous segment of the cytoplasmic domain of mucin-1 to inhibit downstream PI3K-Akt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Sang W Hyun,Akihiro Imamura,Hideharu Ishida,Kurt H Piepenbrink,Simeon E Goldblum,Erik P Lillehoj

Biology of reproduction 103:1110-1120 PubMed32766739

2020

In utero low-protein-diet-programmed type 2 diabetes in adult offspring is mediated by sex hormones in rats†.

Applications

Unspecified application

Species

Unspecified reactive species

Chellakkan S Blesson,Amy K Schutt,Vidyadharan A Vipin,Daren T Tanchico,Pretty R Mathew,Meena Balakrishnan,Ancizar Betancourt,Chandra Yallampalli

FASEB journal : official publication of the Federa 33:12392-12408 PubMed31431059

2019

Susceptibility of microtubuleassociated protein 1 light chain 3β (MAP1LC3B/LC3B) knockout mice to lung injury and fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Vidya Sagar Kesireddy,Shashi Chillappagari,Saket Ahuja,Lars Knudsen,Ingrid Henneke,Johannes Graumann,Silke Meiners,Matthias Ochs,Clemens Ruppert,Martina Korfei,Werner Seeger,Poornima Mahavadi

Histopathology 74:1025-1035 PubMed30725481

2019

The prognostic significance of lysosomal protective protein (cathepsin A) in breast ductal carcinoma in situ.

Applications

Unspecified application

Species

Unspecified reactive species

Michael S Toss,Islam M Miligy,Rita Haj-Ahmad,Kylie L Gorringe,Abdulbaqi AlKawaz,Karuna Mittal,Ian O Ellis,Andrew R Green,Emad A Rakha

The Journal of biological chemistry 294:662-678 PubMed30429216

2018

Neuraminidase 1-mediated desialylation of the mucin 1 ectodomain releases a decoy receptor that protects against lung infection.

Applications

Unspecified application

Species

Unspecified reactive species

Erik P Lillehoj,Wei Guang,Sang W Hyun,Anguo Liu,Nicolas Hegerle,Raphael Simon,Alan S Cross,Hideharu Ishida,Irina G Luzina,Sergei P Atamas,Simeon E Goldblum

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Protective protein/Cathepsin A (PPCA) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protective protein/Cathepsin A (PPCA) antibody (AB217857)

Western blot - Anti-Protective protein/Cathepsin A (PPCA) antibody (AB217857)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise