AB181129

Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal Protective protein/Cathepsin A (PPCA) antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

PPGB, CTSA, Lysosomal protective protein, Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, Protective protein cathepsin A, Protective protein for beta-galactosidase, PPCA

3 Images

Supplier Data

Immunoprecipitation - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] (AB181129)

Detection of Human Protective protein/Cathepsin A (PPCA) by Western Blot of Immunoprecipitate. 293 whole cell lysate loaded, ab181129 used for blotting immunoprecipitated Protective protein/Cathepsin A (PPCA) at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] (ab181129)

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] (AB181129)

All lanes:

Western blot - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] (ab181129) at 1/20000 dilution

Lane 1:

293 lysate at 20 µg

Lane 2:

HeLa lysate at 20 µg

Lane 3:

Jurkat lysate at 20 µg

Lane 4:

HepG2 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

false

Lab

Western blot - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] (AB181129)

Blocking and Diluting buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] (ab181129) at 1/5000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Lane 7:

Rat kidney tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 54 kDa

Observed band size: 34 kDa

false

Exposure time: 180s

Carrier free

Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10434

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protective protein/Cathepsin A (PPCA) also known as cathepsin A or protective proteins is an enzyme with a molecular mass of approximately 54 kDa. The enzyme is expressed widely in different tissues including liver kidney and the central nervous system. Mechanically PPCA plays a role as a carboxypeptidase with serine-type protease activity important for the stabilization and activation of lysosomal enzymes such as beta-galactosidase and neuraminidase. This stabilization is vital for proper lysosomal function and the breakdown of glycoproteins within the cell.

Biological function summary

In conjunction with these enzymes PPCA forms part of the lysosomal multienzyme complex. It regulates the degradation of sialylated glycoconjugates by ensuring the structural integrity and enzymatic activity of lysosomal enzymes. This regulation prevents the accumulation of substrate materials which can disrupt cellular homeostasis. PPCA's interaction with beta-galactosidase and neuraminidase enables the proper catabolism of glycoproteins and glycolipids which is essential for cellular functions.

Pathways

PPCA functions within the lysosomal storage and sialic acid catabolism pathways. These pathways are significant for the degradation and recycling of macromolecules in the lysosome maintaining cellular health. In the sialic acid metabolism pathway PPCA pairs with neuraminidase to break down sialylated substrates while interacting closely with beta-galactosidase in glycoprotein catabolism. These interactions highlight its role in preserving the efficiency of lysosomal degradation processes.

Mutations in the PPCA gene can lead to diseases like galactosialidosis and neuraminidase deficiency. Galactosialidosis arises from defective cathepsin A activity disrupting normal glycoprotein breakdown and resulting in storage disorders. This condition involves both beta-galactosidase and neuraminidase which accumulate as a result of PPCA dysfunction. Similarly neuraminidase deficiency exhibits symptoms due to impaired degradation of sialic acids indicating the necessity of PPCA in disease prevention.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.

See full target information CTSA

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:14173 PubMed35986080

2022

Altered sialidase expression in human myeloid cells undergoing apoptosis and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Sang W Hyun,Chiguang Feng,Anguo Liu,Erik P Lillehoj,Rossana Trotta,Tami J Kingsbury,Antonino Passaniti,Katerina N Lugkey,Sitara Chauhan,John F Cipollo,Irina G Luzina,Sergei P Atamas,Alan S Cross,Simeon E Goldblum

Cell death and differentiation 27:2697-2709 PubMed32447347

2020

Arginine hypomethylation-mediated proteasomal degradation of histone H4-an early biomarker of cellular senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Lin,Hongxin Li,Jiwei Liu,Qianying Hu,Shuai Zhang,Na Zhang,Lingxia Liu,Yingjie Dai,Donghui Cao,Xiaoxue Li,Baiqu Huang,Jun Lu,Yu Zhang

The Journal of biological chemistry 294:662-678 PubMed30429216

2018

Neuraminidase 1-mediated desialylation of the mucin 1 ectodomain releases a decoy receptor that protects against lung infection.

Applications

Unspecified application

Species

Unspecified reactive species

Erik P Lillehoj,Wei Guang,Sang W Hyun,Anguo Liu,Nicolas Hegerle,Raphael Simon,Alan S Cross,Hideharu Ishida,Irina G Luzina,Sergei P Atamas,Simeon E Goldblum

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434]

Immunoprecipitation - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] (AB181129)

Western blot - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] (AB181129)

Western blot - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] (AB181129)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise