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AB181627

Anti-Protein A antibody

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(1 Publication)

Goat Polyclonal Protein A antibody. Suitable for WB, IHC-P and reacts with Staphylococcus aureus samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Staphylococcus aureus subsp. aureus NCTC 8325 spa.

View Alternative Names

SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA

1 Images
Western blot - Anti-Protein A antibody (AB181627)
  • WB

Supplier Data

Western blot - Anti-Protein A antibody (AB181627)

All lanes:

Western blot - Anti-Protein A antibody (ab181627) at 1/5000 dilution

Lane 1:

Protein A reduced at 1 µg

Lane 2:

Protein A reduced at 0.25 µg

Lane 3:

Protein A non reduced at 1 µg

Lane 4:

Protein A non reduced at 0.25 µg

Secondary

All lanes:

DyLight™ 649 donkey anti-goat (pre-adsorbed) secondary antibody at 1/10000 dilution

Predicted band size: 56 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Staphylococcus aureus

Applications

IHC-P, WB

applications

Immunogen

Native Full Length Protein corresponding to Staphylococcus aureus subsp. aureus NCTC 8325 spa.

P02976

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Staphylococcus aureus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification technique
Ion exchange chromatography
Purification notes
Protein A Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
See full target information spa
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 10: PubMed31164467

2019

Candida albicans Augments Staphylococcus aureus Virulence by Engaging the Staphylococcal Quorum Sensing System.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia A Todd,Paul L Fidel,Janette M Harro,Jamese J Hilliard,Christine Tkaczyk,Bret R Sellman,Mairi C Noverr,Brian M Peters
View all publications
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