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AB19483

Anti-Protein A antibody

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(4 Publications)

Anti-Protein A antibody (ab19483) is a chicken polyclonal antibody detecting Protein A in Western Blot, ELISA. Suitable for Staphylococcus aureus.

- Trusted since 2005

View Alternative Names

SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

No

Reacts with

Staphylococcus aureus

Applications

WB, sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Staphylococcus aureus subsp. aureus NCTC 8325 spa.

P02976

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Staphylococcus aureus": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>50 kDa band is due to cleavage of the signal peptide and propeptide</p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>ab19483 can work in direct ELISA, but not indirect ELISA (because any secondary antibody would bind to protein A directly as well as the primary antibody, thus leading to high background – due to the nature of protein A). <a href='/en-us/products/primary-antibodies/hrp-protein-a-antibody-ab18596'>ab18596</a> as detection antibody for sELISA.</p>" } } }

Product details

What is this antibody validated in?
Anti-Protein A antibody (ab19483) is a chicken polyclonal antibody and is validated for use in Western Blot (WB), ELISA in Staphylococcus aureus samples.

What is the molecular weight of Protein A?
Anti-Protein A (ab19483) specifically detects a band for Protein A (UniProt: P02976) at a molecular weight of 56kDa.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
See full target information spa

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Analytical chemistry 96:9676-9683 PubMed38813952

2024

Surface Acoustic Wave-Driven Enhancement of Enzyme-Linked Immunosorbent Assays: ELISAW.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Shuai Zhang,Cécile Floer,Sreeya Anjana Raj Kantubuktha,María José González Ruiz Velasco,James Friend

PLoS biology 22:e3002508 PubMed38377076

2024

Proteins that carry dual targeting signals can act as tethers between peroxisomes and partner organelles.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Bittner,Thorsten Stehlik,Jason Lam,Lazar Dimitrov,Thomas Heimerl,Isabelle Schöck,Jannik Harberding,Anita Dornes,Nikola Heymons,Gert Bange,Maya Schuldiner,Einat Zalckvar,Michael Bölker,Randy Schekman,Johannes Freitag

Nucleic acids research 51:2117-2136 PubMed36715322

2023

Structure and functional determinants of Rad6-Bre1 subunits in the histone H2B ubiquitin-conjugating complex.

Applications

Unspecified application

Species

Unspecified reactive species

Prakash K Shukla,Jesse E Bissell,Sanjit Kumar,Srijana Pokhrel,Sowmiya Palani,Kaitlin S Radmall,Onyeka Obidi,Timothy J Parnell,Julia Brasch,Dennis C Shrieve,Mahesh B Chandrasekharan

F1000Research 8:108 PubMed31275563

2019

Broad-spectrum capture of clinical pathogens using engineered Fc-mannose-binding lectin enhanced by antibiotic treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin T Seiler,Mark Cartwright,Alexandre L M Dinis,Shannon Duffy,Patrick Lombardo,David Cartwright,Elana H Super,Jacqueline Lanzaro,Kristen Dugas,Michael Super,Donald E Ingber
View all publications

Product promise

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