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AB131251

Anti-Protein C antibody

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(1 Review)

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(1 Publication)

Rabbit Polyclonal Protein C antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PROC.

View Alternative Names

Vitamin K-dependent protein C, Anticoagulant protein C, Autoprothrombin IIA, Blood coagulation factor XIV, PROC

2 Images
Flow Cytometry (Intracellular) - Anti-Protein C antibody (AB131251)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Protein C antibody (AB131251)

Overlay histogram showing A549 cells stained with ab131251 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with ab131251 1μg/1x106 cells for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG 5-10 μg/1x106 cells was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody (AB131251)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody (AB131251)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Protein C using ab131251 at 1μg/ml in human mammary cancer tissues.

Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human PROC. The exact immunogen used to generate this antibody is proprietary information.

P04070

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Alternative Products

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1-3 µg/cells for 6 µg/cells", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein C is a vitamin K-dependent serine protease enzyme that plays an important role in blood coagulation. It is also known as autoprothrombin IIA or blood coagulation factor XIV. The molecular weight of Protein C is approximately 62 kDa. It is synthesized in the liver and circulates in the plasma as an inactive zymogen. The protein becomes activated on the surface of endothelial cells and platelets where it plays an anticoagulant role.
Biological function summary

Protein C serves as an important regulator in the coagulation pathway. Upon activation it joins with a cofactor known as Protein S to form the activated Protein C complex which inactivates clotting factors Va and VIIIa. This inactivation leads to a reduction in thrombin formation thereby preventing excessive blood clotting. The activated C complex works efficiently on phospholipid surfaces such as those found on cellular membranes.

Pathways

Protein C activation is an important step in the anticoagulant pathway and works to balance the procoagulant actions of other factors. Protein C functions in tandem with proteins such as thrombin which transforms it into its active form when bound to thrombomodulin on the endothelial cell surface. The activated Protein C pathway ensures proper blood flow by preventing unchecked clot propagation and is intricately tied with pathways involving thrombin and factors Va and VIIIa.

Protein C deficiency is associated with an increased risk of developing thromboembolic disorders like deep vein thrombosis and pulmonary embolism. Deficiency can be congenital or acquired resulting in uncontrolled coagulation and increased susceptibility to clot formation. Additionally impaired activity of protein C is related to a disorder known as purpura fulminans which is characterized by blood clot formation and skin necrosis. The relation of Protein C to these conditions often involves dysfunctional interactions with factors Va VIIIa and thrombin.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed : 25618265, PubMed : 39880037). Exerts a protective effect on the endothelial cell barrier function (PubMed : 25651845).
See full target information PROC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Archives of pathology & laboratory medicine 147:797-807 PubMed36191342

2022

Clinicopathologic Impact of Peptide Hormonal Expression in Rectal Neuroendocrine Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Jisup Kim,Dong-Hoon Yang,HaeSung Jung,HyungJun Cho,Hyeung-Jin Jang,Changhoon Yoo,In Ja Park,Baek-Yeol Ryoo,Jin-Sook Ryu,Seung-Mo Hong
View all publications
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Product promise

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