Anti-Protein C antibody [EPR28195-90] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387)

This data was developed using ab313386, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling Protein C with ab313386 at 1/500 (1.038 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse skeletal muscle.The section was incubated with ab313386 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387)

This data was developed using ab313386, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Protein C with ab313386 at 1/500 (1.038 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse cerebrum.The section was incubated with ab313386 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387)

This data was developed using ab313386, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Non-perfused fixed mouse cardiac muscle tissue labeling Protein C with ab313386 at 1/500 (1.038 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on plasma in non-perfused fixed mouse cardiac muscleThe section was incubated with ab313386 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387)

This data was developed using ab313386, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Protein C with ab313386 at 1/500 (1.038 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver.The section was incubated with ab313386 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387)

This data was developed using ab313386, the same antibody clone in a different buffer formulation. Protein C was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate with ab313386 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313386 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate Lane 2 : ab313386 IP in Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313386 in Hepa1-6 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 41 seconds

All lanes:

Immunoprecipitation - Anti-Protein C antibody [EPR28195-90] (<a href='/en-us/products/primary-antibodies/protein-c-antibody-epr28195-90-ab313386'>ab313386</a>) at 1/30 dilution

All lanes:

Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 41s

• WB

Supplier Data

Western blot - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387)

This data was developed using ab313386, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : brain, skeletal muscle, colon. The identity of the lower MW band at approximately 20 kDa and the higher MW band at approximately 125 kDa are unknown. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 26 seconds.

All lanes:

Western blot - Anti-Protein C antibody [EPR28195-90] (<a href='/en-us/products/primary-antibodies/protein-c-antibody-epr28195-90-ab313386'>ab313386</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 4:

Mouse colon tissue lysate at 20 µg

Lane 5:

Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 62 kDa

false

Exposure time: 26s