Rabbit Recombinant Monoclonal Protein C antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Mouse samples.
Images
![Western blot - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387), expandable thumbnail](https://content.abcam.com/products/images/protein-c-antibody-epr28195-90-bsa-and-azide-free-ab313387--western-blot-img418201.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387)")
![Immunoprecipitation - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387), expandable thumbnail](https://content.abcam.com/products/images/protein-c-antibody-epr28195-90-bsa-and-azide-free-ab313387--immunoprecipitation-img418231.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387), expandable thumbnail](https://content.abcam.com/products/images/protein-c-antibody-epr28195-90-bsa-and-azide-free-ab313387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418260.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387), expandable thumbnail](https://content.abcam.com/products/images/protein-c-antibody-epr28195-90-bsa-and-azide-free-ab313387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418259.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387), expandable thumbnail](https://content.abcam.com/products/images/protein-c-antibody-epr28195-90-bsa-and-azide-free-ab313387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418258.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (AB313387)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IP | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.
Alternative names
Vitamin K-dependent protein C, Anticoagulant protein C, Autoprothrombin IIA, Blood coagulation factor XIV, Proc
Recommended products
Rabbit Recombinant Monoclonal Protein C antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28195-90
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein C is a vitamin K-dependent serine protease enzyme that plays an important role in blood coagulation. It is also known as autoprothrombin IIA or blood coagulation factor XIV. The molecular weight of Protein C is approximately 62 kDa. It is synthesized in the liver and circulates in the plasma as an inactive zymogen. The protein becomes activated on the surface of endothelial cells and platelets where it plays an anticoagulant role.
- Biological function summary
Protein C serves as an important regulator in the coagulation pathway. Upon activation it joins with a cofactor known as Protein S to form the activated Protein C complex which inactivates clotting factors Va and VIIIa. This inactivation leads to a reduction in thrombin formation thereby preventing excessive blood clotting. The activated C complex works efficiently on phospholipid surfaces such as those found on cellular membranes.
- Pathways
Protein C activation is an important step in the anticoagulant pathway and works to balance the procoagulant actions of other factors. Protein C functions in tandem with proteins such as thrombin which transforms it into its active form when bound to thrombomodulin on the endothelial cell surface. The activated Protein C pathway ensures proper blood flow by preventing unchecked clot propagation and is intricately tied with pathways involving thrombin and factors Va and VIIIa.
- Associated diseases and disorders
Protein C deficiency is associated with an increased risk of developing thromboembolic disorders like deep vein thrombosis and pulmonary embolism. Deficiency can be congenital or acquired resulting in uncontrolled coagulation and increased susceptibility to clot formation. Additionally impaired activity of protein C is related to a disorder known as purpura fulminans which is characterized by blood clot formation and skin necrosis. The relation of Protein C to these conditions often involves dysfunctional interactions with factors Va VIIIa and thrombin.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387), expandable thumbnail](https://content.abcam.com/products/images/protein-c-antibody-epr28195-90-bsa-and-azide-free-ab313387--western-blot-img418201.jpg "Western blot - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)")
Western blot - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)
This data was developed using Anti-Protein C antibody [EPR28195-90] ab313386, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: brain, skeletal muscle, colon.
The identity of the lower MW band at approximately 20 kDa and the higher MW band at approximately 125 kDa are unknown.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 26 seconds.All lanes: Western blot - Anti-Protein C antibody [EPR28195-90] (Anti-Protein C antibody [EPR28195-90] ab313386) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse skeletal muscle tissue lysate at 20 µg
Lane 4: Mouse colon tissue lysate at 20 µg
Lane 5: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 62 kDa
Exposure time: 26s
![Immunoprecipitation - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387), expandable thumbnail](https://content.abcam.com/products/images/protein-c-antibody-epr28195-90-bsa-and-azide-free-ab313387--immunoprecipitation-img418231.jpg "Immunoprecipitation - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)")
Immunoprecipitation - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)
This data was developed using Anti-Protein C antibody [EPR28195-90] ab313386, the same antibody clone in a different buffer formulation.
Protein C was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate with Anti-Protein C antibody [EPR28195-90] ab313386 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Protein C antibody [EPR28195-90] ab313386 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate
Lane 2: Anti-Protein C antibody [EPR28195-90] ab313386 IP in Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Protein C antibody [EPR28195-90] ab313386 in Hepa1-6 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 secondsAll lanes: Immunoprecipitation - Anti-Protein C antibody [EPR28195-90] (Anti-Protein C antibody [EPR28195-90] ab313386) at 1/30 dilution
All lanes: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 41s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387), expandable thumbnail](https://content.abcam.com/products/images/protein-c-antibody-epr28195-90-bsa-and-azide-free-ab313387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418260.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)
This data was developed using Anti-Protein C antibody [EPR28195-90] ab313386, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Protein C with Anti-Protein C antibody [EPR28195-90] ab313386 at 1/500 (1.038 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse cerebrum.The section was incubated with Anti-Protein C antibody [EPR28195-90] ab313386 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387), expandable thumbnail](https://content.abcam.com/products/images/protein-c-antibody-epr28195-90-bsa-and-azide-free-ab313387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418259.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)
This data was developed using Anti-Protein C antibody [EPR28195-90] ab313386, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling Protein C with Anti-Protein C antibody [EPR28195-90] ab313386 at 1/500 (1.038 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse skeletal muscle.The section was incubated with Anti-Protein C antibody [EPR28195-90] ab313386 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387), expandable thumbnail](https://content.abcam.com/products/images/protein-c-antibody-epr28195-90-bsa-and-azide-free-ab313387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418258.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)
This data was developed using Anti-Protein C antibody [EPR28195-90] ab313386, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Protein C with Anti-Protein C antibody [EPR28195-90] ab313386 at 1/500 (1.038 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver.The section was incubated with Anti-Protein C antibody [EPR28195-90] ab313386 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387), expandable thumbnail](https://content.abcam.com/products/images/protein-c-antibody-epr28195-90-bsa-and-azide-free-ab313387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418257.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)
This data was developed using Anti-Protein C antibody [EPR28195-90] ab313386, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Non-perfused fixed mouse cardiac muscle tissue labeling Protein C with Anti-Protein C antibody [EPR28195-90] ab313386 at 1/500 (1.038 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on plasma in non-perfused fixed mouse cardiac muscleThe section was incubated with Anti-Protein C antibody [EPR28195-90] ab313386 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387), expandable thumbnail](https://content.abcam.com/products/images/protein-c-antibody-epr28195-90-bsa-and-azide-free-ab313387--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418257.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody [EPR28195-90] - BSA and Azide free (ab313387)")
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