Mouse Monoclonal Protein CASP antibody. Carrier free. Suitable for Flow Cyt, WB, sELISA, IHC-P, ICC/IF and reacts with Human, Transfected cell lysate - Human, Recombinant fragment samples. Cited in 24 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CUX1 aa 500-650.
Images
![Flow Cytometry - Anti-Protein CASP antibody [2A10] - BSA and Azide free (AB54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--flow-cytometry-img50998.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Protein CASP antibody [2A10] - BSA and Azide free (AB54583)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (AB54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84423.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (AB54583)")
![Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [2A10] - BSA and Azide free (AB54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--immunocytochemistry-immunofluorescence-img121285.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [2A10] - BSA and Azide free (AB54583)")
![Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (AB54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--western-blot-img69136.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (AB54583)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (AB54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23930.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (AB54583)")
Publications
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- Fluids and barriers of the CNS 20:452023Age-appropriate potassium clearance from perinatal cerebrospinal fluid depends on choroid plexus NKCC1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyann M Fame,Huixin Xu,Aja Pragana,Maria LehtinenPubMed 37328833
- Journal of virology 97:e00144232023SARS-CoV-2 Infection of Human Neurons Is TMPRSS2 Independent, Requires Endosomal Cell Entry, and Can Be Blocked by Inhibitors of Host Phosphoinositol-5 Kinase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPinja Kettunen,Angelina Lesnikova,Noora Räsänen,Ravi Ojha,Leena Palmunen,Markku Laakso,Šárka Lehtonen,Johanna Kuusisto,Olli Pietiläinen,Saber H Saber,Merja Joensuu,Olli P Vapalahti,Jari Koistinaho,Taisia Rolova,Giuseppe BalistreriPubMed 37039676
- Nature cell biology 24:981-9952022Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Rosebrock,Sneha Arora,Naresh Mutukula,Rotem Volkman,Elzbieta Gralinska,Anastasios Balaskas,Amèlia Aragonés Hernández,René Buschow,Björn Brändl,Franz-Josef Müller,Peter F Arndt,Martin Vingron,Yechiel ElkabetzPubMed 35697781
- Frontiers in pediatrics 10:8812872022Identification of SOX2 Interacting Proteins in the Developing Mouse Lung With Potential Implications for Congenital Diaphragmatic Hernia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKim A A Schilders,Gabriëla G Edel,Evelien Eenjes,Bianca Oresta,Judith Birkhoff,Anne Boerema-de Munck,Marjon Buscop-van Kempen,Panagiotis Liakopoulos,Petros Kolovos,Jeroen A A Demmers,Raymond Poot,Rene M H Wijnen,Dick Tibboel,Robbert J RottierPubMed 35615634
- Nature communications 13:23872022Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabio Papes,Antonio P Camargo,Janaina S de Souza,Vinicius M A Carvalho,Ryan A Szeto,Erin LaMontagne,José R Teixeira,Simoni H Avansini,Sandra M Sánchez-Sánchez,Thiago S Nakahara,Carolina N Santo,Wei Wu,Hang Yao,Barbara M P Araújo,Paulo E N F Velho,Gabriel G Haddad,Alysson R MuotriPubMed 35501322
- Cancers 13:2021CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeidi Griesmann,Sebastian Mühl,Jan Riedel,Katharina Theuerkorn,Bence Sipos,Irene Esposito,Gregory B Vanden Heuvel,Patrick MichlPubMed 34070180
- Nature communications 12:26782021Elp2 mutations perturb the epitranscriptome and lead to a complex neurodevelopmental phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarija Kojic,Tomasz Gawda,Monika Gaik,Alexander Begg,Anna Salerno-Kochan,Nyoman D Kurniawan,Alun Jones,Katarzyna Drożdżyk,Anna Kościelniak,Andrzej Chramiec-Głąbik,Soroor Hediyeh-Zadeh,Maria Kasherman,Woo Jun Shim,Enakshi Sinniah,Laura A Genovesi,Rannvá K Abrahamsen,Christina D Fenger,Camilla G Madsen,Julie S Cohen,Ali Fatemi,Zornitza Stark,Sebastian Lunke,Joy Lee,Jonas K Hansen,Martin F Boxill,Boris Keren,Isabelle Marey,Margarita S Saenz,Kathleen Brown,Suzanne A Alexander,Sergey Mureev,Alina Batzilla,Melissa J Davis,Michael Piper,Mikael Bodén,Thomas H J Burne,Nathan J Palpant,Rikke S Møller,Sebastian Glatt,Brandon J WainwrightPubMed 33976153
- The Journal of neuroscience : the official journal of the Society for Neuroscience 41:4335-43482021Continuous Monitoring of Tau-Induced Neurotoxicity in Patient-Derived iPSC-Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDerek H Oakley,Naomi Klickstein,Caitlin Commins,Mirra Chung,Simon Dujardin,Rachel E Bennett,Bradley T Hyman,Matthew P FroschPubMed 33893219
- PloS one 16:e02480002021A proteomics approach for the identification of cullin-9 (CUL9) related signaling pathways in induced pluripotent stem cell models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalya A Ortolano,Alejandra I Romero-Morales,Megan L Rasmussen,Caroline Bodnya,Leigh A Kline,Piyush Joshi,Jon P Connelly,Kristie L Rose,Shondra M Pruett-Miller,Vivian GamaPubMed 33705438
- Frontiers in immunology 12:6048122021Hyperactive Behavior and Altered Brain Morphology in Adult Complement C3a Receptor Deficient Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Pozo-Rodrigálvarez,Roosa Ollaranta,Jenny Skoog,Milos Pekny,Marcela PeknaPubMed 33692783
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CUX1 aa 500-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q13948
Reactivity data
Select an application| Flow Cyt | WB | sELISA | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested |
| Recombinant fragment | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Transfected cell lysate - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Recombinant fragment | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Recombinant fragment | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Recombinant fragment | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
May be involved in intra-Golgi retrograde transport.
Alternative names
CUTL1, CUX1, Protein CASP
Recommended products
Mouse Monoclonal Protein CASP antibody. Carrier free. Suitable for Flow Cyt, WB, sELISA, IHC-P, ICC/IF and reacts with Human, Transfected cell lysate - Human, Recombinant fragment samples. Cited in 24 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CUX1 aa 500-650.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CUX1 aa 500-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q13948
- Carrier free
- Yes
- Clone number
- 2A10
- Purity
- Tissue culture supernatant
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 13th Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein CASP also known as Caspase or CASP protein is an important player in the process of apoptosis. Caspases are cysteine-aspartic proteases that exist as inactive proenzymes made of a prodomain large and small subunits. Upon activation caspases cleave substrates after aspartic acid residues. The Caspase family proteins have similar structures with molecular masses around 20 kDa to 40 kDa. CASP proteins are widely expressed across different tissues in the body. Activation can happen through intrinsic and extrinsic pathways enabling them to mediate the orderly dismantling of cellular components.
- Biological function summary
Protein CASP acts as an executioner enzyme in cell death. It forms part of intricate protein complexes notably the apoptosome which includes APAF1 and cytochrome c. Once activated CASP proteins initiate a cascade of proteolytic events that lead to the dismantling of the cell. This process is tightly regulated and is essential for maintaining homeostasis and tissue development. Other caspases work upstream to activate downstream executioner caspases further highlighting the protein's role in these biological functions.
- Pathways
Protein CASP plays a significant role in apoptotic signaling pathways. It is pivotal in both the extrinsic and intrinsic apoptotic pathways. In the extrinsic pathway proteins such as FADD and DISC interact with CASP-8 to trigger apoptosis. The intrinsic pathway includes the release of cytochrome c which partners with APAF1 leading to the activation of CASP-9 and subsequently other caspases. This cascade illustrates the interconnected nature of apoptotic signaling with other molecular pathways ensuring the precise regulation of cell death.
- Associated diseases and disorders
Protein CASP is associated with cancer and neurodegenerative diseases. Dysregulation of CASP activity can result in either excessive cell death or survival contributing to disease pathogenesis. In cancers often CASP activity is impaired allowing cancer cells to evade apoptosis and proliferate unchecked. In neurodegenerative disorders such as Alzheimer's abnormal CASP activation contributes to neuronal loss. The interaction between CASP proteins and BCL-2 family members highlights their role in these diseases emphasizing the therapeutic targeting potential in modulating CASP activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Flow Cytometry - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--flow-cytometry-img50998.jpg "Flow Cytometry - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)")
Flow Cytometry - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)
Overlay histogram showing MCF7 (human breast adenocarcinoma cell line) cells stained with ab54583 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3 M glycine to block non-specific protein-protein interactions followed by the antibody (ab54583, 1 μg/1 x 106 cells) for 30 minutes at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2 μg/1 x 106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84423.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)
Formalin-fixed, paraffin-embedded human spleen tissue stained for Protein CASP with ab54583 at 5 μg/ml in immunohistochemical analysis.
This image was generated using the ascites version of the product.
![Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--immunocytochemistry-immunofluorescence-img121285.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)")
Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Protein CASP (green) using ab54583 at 10 μg/ml in ICC/IF.
This image was generated using the ascites version of the product.
![Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--western-blot-img69136.jpg "Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)")
Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583) at 5 µg/mL
Lane 1: Protein CASP transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 77 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23930.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)
Formalin-fixed, paraffin-embedded human malignant lymphoma, diffuse large B tissue stained for Protein CASP with ab54583 at 5 μg/ml in immunohistochemical analysis.
This image was generated using the ascites version of the product.
![Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--western-blot-img136495.jpg "Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)")
Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Expected MW 38 kDa.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583) at 5 µg/mL
All lanes: Tagged recombinant Protein CASP protein
Predicted band size: 114 kDa, 167 kDa, 185 kDa, 217 kDa, 22 kDa, 41 kDa, 629 kDa, 70 kDa, 71 kDa, 77 kDa, 83 kDa
![Sandwich ELISA - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--sandwich-elisa-img70841.jpg "Sandwich ELISA - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)")
Sandwich ELISA - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)
Detection limit of ab54583 is approximately 0.03 ng/ml as a capture antibody.
This image was generated using the ascites version of the product.
![Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--western-blot-img136495.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)")
![Sandwich ELISA - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583), expandable thumbnail](https://content.abcam.com/products/images/protein-casp-antibody-2a10-bsa-and-azide-free-ab54583--sandwich-elisa-img70841.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)")
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