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Rabbit Recombinant Monoclonal Protein CASP antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.


Images

Western blot - Anti-Protein CASP antibody [EPR18806] (AB182216), expandable thumbnail
  • Western blot - Anti-Protein CASP antibody [EPR18806] (AB182216), expandable thumbnail
  • Western blot - Anti-Protein CASP antibody [EPR18806] (AB182216), expandable thumbnail
  • Western blot - Anti-Protein CASP antibody [EPR18806] (AB182216), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [EPR18806] (AB182216), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Expected
Tested
Tested
Tested
Not recommended
Rat
Expected
Tested
Expected
Expected
Not recommended

Tested
Tested

Species

Human

Dilution info

1/40

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

-

Species

Rat

Dilution info

1/2000

Notes

-

Species

Human

Dilution info

1/2000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

ab182216 binds weakly in ICC

Species

Human

Dilution info

1/100

Notes

ab182216 binds weakly in ICC

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/120

Notes

-

Species

Human

Dilution info

1/120

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

May be involved in intra-Golgi retrograde transport.

Alternative names

CUTL1, CUTL1, CUX1, Protein CASP

Recommended products

Rabbit Recombinant Monoclonal Protein CASP antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18806

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Protein CASP also known as Caspase or CASP protein is an important player in the process of apoptosis. Caspases are cysteine-aspartic proteases that exist as inactive proenzymes made of a prodomain large and small subunits. Upon activation caspases cleave substrates after aspartic acid residues. The Caspase family proteins have similar structures with molecular masses around 20 kDa to 40 kDa. CASP proteins are widely expressed across different tissues in the body. Activation can happen through intrinsic and extrinsic pathways enabling them to mediate the orderly dismantling of cellular components.

Biological function summary

Protein CASP acts as an executioner enzyme in cell death. It forms part of intricate protein complexes notably the apoptosome which includes APAF1 and cytochrome c. Once activated CASP proteins initiate a cascade of proteolytic events that lead to the dismantling of the cell. This process is tightly regulated and is essential for maintaining homeostasis and tissue development. Other caspases work upstream to activate downstream executioner caspases further highlighting the protein's role in these biological functions.

Pathways

Protein CASP plays a significant role in apoptotic signaling pathways. It is pivotal in both the extrinsic and intrinsic apoptotic pathways. In the extrinsic pathway proteins such as FADD and DISC interact with CASP-8 to trigger apoptosis. The intrinsic pathway includes the release of cytochrome c which partners with APAF1 leading to the activation of CASP-9 and subsequently other caspases. This cascade illustrates the interconnected nature of apoptotic signaling with other molecular pathways ensuring the precise regulation of cell death.

Associated diseases and disorders

Protein CASP is associated with cancer and neurodegenerative diseases. Dysregulation of CASP activity can result in either excessive cell death or survival contributing to disease pathogenesis. In cancers often CASP activity is impaired allowing cancer cells to evade apoptosis and proliferate unchecked. In neurodegenerative disorders such as Alzheimer's abnormal CASP activation contributes to neuronal loss. The interaction between CASP proteins and BCL-2 family members highlights their role in these diseases emphasizing the therapeutic targeting potential in modulating CASP activity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-Protein CASP antibody [EPR18806] (ab182216), expandable thumbnail

    Western blot - Anti-Protein CASP antibody [EPR18806] (ab182216)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Protein CASP antibody [EPR18806] (ab182216) at 1/2000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 77 kDa

    Observed band size: 77 kDa

    Exposure time: 30s

  • Western blot - Anti-Protein CASP antibody [EPR18806] (ab182216), expandable thumbnail

    Western blot - Anti-Protein CASP antibody [EPR18806] (ab182216)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 30 seconds; Lane 3: 3 minutes.

    All lanes: Western blot - Anti-Protein CASP antibody [EPR18806] (ab182216) at 1/2000 dilution

    Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

    Lane 2: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg

    Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 77 kDa

    Observed band size: 77 kDa

  • Western blot - Anti-Protein CASP antibody [EPR18806] (ab182216), expandable thumbnail

    Western blot - Anti-Protein CASP antibody [EPR18806] (ab182216)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane1,2,3 and 4: 3 minutes; Lane 5,6 and 7: 30 seconds.

    All lanes: Western blot - Anti-Protein CASP antibody [EPR18806] (ab182216) at 1/2000 dilution

    Lane 1: Rat testis lysate at 20 µg

    Lane 2: Rat cerebral cortex lysate at 20 µg

    Lane 3: Mouse cerebral cortex lysate at 20 µg

    Lane 4: Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg

    Lane 5: Mouse testis lysate at 20 µg

    Lane 6: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 7: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 77 kDa

    Observed band size: 77 kDa

  • Western blot - Anti-Protein CASP antibody [EPR18806] (ab182216), expandable thumbnail

    Western blot - Anti-Protein CASP antibody [EPR18806] (ab182216)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-6: 30 seconds; Lane 7,8,9 and 10: 8 seconds.

    All lanes: Western blot - Anti-Protein CASP antibody [EPR18806] (ab182216) at 1/2000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse kidney lysate at 10 µg

    Lane 3: Mouse spleen lysate at 10 µg

    Lane 4: Rat brain lysate at 10 µg

    Lane 5: Rat heart lysate at 10 µg

    Lane 6: Rat spleen lysate at 10 µg

    Lane 7: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 8: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 9: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Lane 10: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 77 kDa, 85 kDa

    Observed band size: 77 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [EPR18806] (ab182216), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [EPR18806] (ab182216)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Protein CASP with ab182216 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing specific but weak cytoplasmic staining on NIH/3T3 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:

    -ve control 1: Anti-ZIP Kinase antibody [EPR18809-86] ab210528 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [EPR18806] (ab182216), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [EPR18806] (ab182216)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Protein CASP with ab182216 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing specific but weak cytoplasmic staining on U-87 MG cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:

    -ve control 1: Anti-ZIP Kinase antibody [EPR18809-86] ab210528 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Immunoprecipitation - Anti-Protein CASP antibody [EPR18806] (ab182216), expandable thumbnail

    Immunoprecipitation - Anti-Protein CASP antibody [EPR18806] (ab182216)

    Protein CASP was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab182216 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab182216 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate, 10μg (Input).

    Lane 2: ab182216 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit IgG, [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab182216 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-Protein CASP antibody [EPR18806] (ab182216)

    Predicted band size: 77 kDa

  • Flow Cytometry (Intracellular) - Anti-Protein CASP antibody [EPR18806] (ab182216), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Protein CASP antibody [EPR18806] (ab182216)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Protein CASP with ab182216 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Protein CASP antibody [EPR18806] (ab182216), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Protein CASP antibody [EPR18806] (ab182216)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Protein CASP with ab182216 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal[EPR25A] -Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

    Note:Cells were permeabilized with 90% methanol (in 1XPBS).

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