Rabbit Polyclonal Protein phosphatase 1 inhibitor subunit 2 phospho T72 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PPP1R2 phospho T72.
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- Methods in molecular biology (Clifton, N.J.) 1619:263-3012017Surface Profiling of Extracellular Vesicles from Plasma or Ascites Fluid Using DotScan Antibody Microarrays.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLarissa Belov,Susannah Hallal,Kieran Matic,Jerry Zhou,Sandra Wissmueller,Nuzhat Ahmed,Sumaiya Tanjil,Stephen P Mulligan,O Giles Best,Richard J Simpson,Richard I ChristophersonPubMed 28674892
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PPP1R2 phospho T72. The exact immunogen used to generate this antibody is proprietary information. Database link P41236
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Inhibitor of protein-phosphatase 1.
Alternative names
IPP2, PPP1R2, Protein phosphatase inhibitor 2, IPP-2
Recommended products
Rabbit Polyclonal Protein phosphatase 1 inhibitor subunit 2 phospho T72 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PPP1R2 phospho T72.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PPP1R2 phospho T72. The exact immunogen used to generate this antibody is proprietary information. Database link P41236
- Purification technique
- Affinity purification Immunogen
- Specificity
ab27850 recognises Inhibitor-2.
- Concentration
- Purification notes
The antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Inhibitor-2. The final product is generated by affinity chromatography using an Inhibitor-2 -derived peptide that is phosphorylated at threonine 72.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Protein Phosphatase 1 Inhibitor Subunit 2 often abbreviated as PPP1R2 functions as a regulatory protein that inhibits the activity of Protein Phosphatase 1 (PP1). PP1 is an important enzyme in various cellular processes including cell division and glycogen metabolism. PPP1R2 has a molecular mass of approximately 20 kDa. This inhibitory protein is ubiquitously expressed throughout different tissues indicating its role in fundamental cellular functions.
- Biological function summary
PPP1R2 plays a critical role in cellular regulation by modulating PP1 activity. It forms a complex with PP1 in which PPP1R2 binds directly to the catalytic subunit of PP1 inhibiting its phosphatase activity. By controlling PP1 activity PPP1R2 impacts processes like muscle contraction protein synthesis and neuronal signaling making it an active participant in maintaining cellular homeostasis.
- Pathways
PPP1R2 functions within important signaling and dephosphorylation pathways. It is notably involved in the glycogen metabolism pathway where it regulates PP1 activity influencing glycogen synthesis and breakdown. Additionally it participates in the cell cycle regulation pathway interacting with other proteins such as PP1 to control cell proliferation and division. The regulation of these pathways by PPP1R2 highlights its importance in key cellular activities.
- Associated diseases and disorders
PPP1R2's dysregulation can associate with several conditions. For instance alterations in PPP1R2 levels have implications in neurodegenerative diseases like Alzheimer's Disease since it affects neuronal protein dephosphorylation. Furthermore abnormal PPP1R2 expression can link to metabolic disorders including Type 2 Diabetes due to its role in glycogen metabolism. In these diseases its interaction with other proteins like PP1 and insulin signaling components is significant highlighting its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
All lanes: Western blot - Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate at 30 µg
Lane 2: HT 29 whole cell lysate at 30 µg
Lane 3: Hel 92.1.7 whole cell lysate at 30 µg
Lane 4: HeLa whole cell lysate at 30 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Secondary Antibody at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 32 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
Immunocytochemistry/Immunofluorescent analysis of A431 cells treated with Nocodazole for 24 hours, staining Protein phosphatase 1 inhibitor subunit 2 at 1/250 dilution, followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X 100 for ten minutes, and blocked with 1% BSA for one hour at room temperature. Nuclei were counterstained with DAPI (blue). F-actin was stained with Rhodamine Phalloidin (red).
Negative controls are untreated A431 cells (e) and a secondary only antibody control (f).
Immunocytochemistry/ Immunofluorescence - Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
Immunocyutochemistry/Immunofluroescence analysis of HeLa cells staining Protein phosphatase 1 inhibitor subunit 2 with ab27850 (green). Cells were grown on a chamber slide and fixed in cold 10% trichloroacetic acid for 10 minutes, rinsed in TBS and permeabilized with 0.1% Triton-X-100 for 15 minutes.
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