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AB234671

Anti-Protein PrgJ antibody

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(1 Publication)

Rabbit Polyclonal Protein PrgJ antibody. Suitable for WB and reacts with Recombinant full length protein - Salmonella typhimurium samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 prgJ.

View Alternative Names

STM2872, prgJ, Protein PrgJ

1 Images
Western blot - Anti-Protein PrgJ antibody (AB234671)
  • WB

Supplier Data

Western blot - Anti-Protein PrgJ antibody (AB234671)

All lanes:

Western blot - Anti-Protein PrgJ antibody (ab234671) at 1/500 dilution

Lane 1:

Recombinant Salmonella typhimurium PrgJ protein, 80 ng

Lane 2:

Recombinant Salmonella typhimurium PrgJ protein, 40 ng

Lane 3:

Recombinant Salmonella typhimurium PrgJ protein, 20 ng

Lane 4:

Recombinant Salmonella typhimurium PrgJ protein, 10 ng

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 prgJ.

P41785

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Salmonella typhimurium": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein PrgJ sometimes referred to as PrgJ serves as an important component of the type III secretion system (T3SS) in certain pathogenic bacteria such as Salmonella and Shigella species. It plays an important mechanical role in forming the needle-like structure that allows these bacteria to inject virulence factors directly into host cells. PrgJ is located in the cytoplasm and sometimes partially in the bacterial membrane. Its molecular mass is approximately 13 kDa making it a small yet functional protein important for structural assembly.
Biological function summary

The assembly of the T3SS involves PrgJ working together with other proteins within the system. PrgJ forms the inner rod of the needle acting as a scaffold to maintain the structure's integrity and ensuring efficient translocation of effector proteins. It participates in forming a complex along with PrgH PrgK and other components. This complex enables bacteria to interact with eukaryotic cells facilitating the delivery of toxins that can manipulate host cellular processes.

Pathways

PrgJ takes part in the bacterial infection pathway involving the type III secretion system. This protein plays an integral role in the secretion of virulence factors that disrupt host pathways leading to infection. Within this system PrgJ associates closely with proteins like PrgI and InvJ harmonizing their activities to ensure effective protein translocation. Additionally PrgJ involvement in this pathway showcases its relationship with central cellular pathways in host organisms highlighting its significance in microbial pathogenesis.

PrgJ holds association with conditions like gastroenteritis and enteric fever both caused by pathogenic bacteria utilizing T3SS. The presence of PrgJ in pathogens like Salmonella links it to diseases characterized by severe gastrointestinal infection. It interacts with proteins central to bacterial pathogenicity such as SipA and SipC which facilitate cellular invasion and intracellular survival. Understanding PrgJ function and its role within the T3SS offers insight into developing treatments targeting bacterial infections and potentially mitigating associated diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for invasion of epithelial cells.
See full target information prgJ
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Virus research 355:199570 PubMed40188851

2025

Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.

Applications

Unspecified application

Species

Unspecified reactive species

Rupaly Akhter,Kazi Anowar Hossain,Bouchra Kitab,Yoshihiro Sakoda,Kyoko Tsukiyama-Kohara
View all publications
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Product promise

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