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Rabbit Recombinant Monoclonal Protein S antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Protein S antibody [EPR24509-70] (AB280885), expandable thumbnail
  • Immunoprecipitation - Anti-Protein S antibody [EPR24509-70] (AB280885), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] (AB280885), expandable thumbnail
  • Western blot - Anti-Protein S antibody [EPR24509-70] (AB280885), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] (AB280885), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PIHC-FrFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.

Alternative names

PROS, PROS1, Vitamin K-dependent protein S

Recommended products

Rabbit Recombinant Monoclonal Protein S antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24509-70
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Protein S also known as PROS1 is a vitamin K-dependent plasma glycoprotein with a molecular mass of approximately 75 kDa. It is synthesized mainly in the liver but also found in other tissues such as the endothelium megakaryocytes and osteoblasts. This protein plays a significant role in the regulation of blood coagulation by serving as a non-enzymatic cofactor for activated protein C (APC) in the proteolytic degradation of coagulation factors Va and VIIIa.

Biological function summary

The integrated function of Protein S within the coagulation cascade cannot be overemphasized. It acts synergistically with APC to limit thrombin generation therefore facilitating anticoagulation. Protein S is part of a complex with APC that exerts a potent anticoagulant effect. While free Protein S shows functional activity a large fraction circulates in complex with C4b-binding protein where it exhibits limited cofactor activity.

Pathways

The anticoagulant role of Protein S anchors it within the critical pathways like the protein C pathway impacting hemostasis. It directly associates with proteins such as Factor Va and Factor VIIIa and indirectly with proteins like thrombin. The regulation of coagulation through these pathways highlights its contribution to maintaining vascular integrity and preventing thrombosis.

Associated diseases and disorders

Protein S deficiency has clinical implications in thrombotic conditions including deep vein thrombosis (DVT) and pulmonary embolism (PE). Such deficiencies can be hereditary or acquired often linked to disorders affecting vitamin K metabolism or liver function. Protein C another key anticoagulant closely interacts with Protein S in these disorders highlighting their collaborative effort in preventing excessive clot formation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Protein S antibody [EPR24509-70] (ab280885), expandable thumbnail

    Western blot - Anti-Protein S antibody [EPR24509-70] (ab280885)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:28118606, 27447084).

    Negative control: HaCaT (PMID:28118606).

    The blot of lane 1 was developed using a higher sensitivity ECL substrate.

    Exposure time: Lane 1: 92 seconds Lane 2: 48 seconds Lane 3: 26 seconds Lane 4-6: 3 minutes

    All lanes: Western blot - Anti-Protein S antibody [EPR24509-70] (ab280885) at 1/1000 dilution

    Lane 1: Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Human liver tissue lysate at 20 µg

    Lane 3: Human lung tissue lysate at 20 µg

    Lane 4: Human stomach tissue lysate at 20 µg

    Lane 5: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: HaCaT (human skin keratinocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 75 kDa

    Observed band size: 65 kDa, 75 kDa, 85 kDa

  • Immunoprecipitation - Anti-Protein S antibody [EPR24509-70] (ab280885), expandable thumbnail

    Immunoprecipitation - Anti-Protein S antibody [EPR24509-70] (ab280885)

    Protein S was immunoprecipitated from 0.35 mg of Human liver tissue lysate with ab280885 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280885 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human liver tissue lysate 10 ug

    Lane 2: ab280885 IP in Human liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab280885 in human liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

    All lanes: Immunoprecipitation - Anti-Protein S antibody [EPR24509-70] (ab280885)

    Predicted band size: 75 kDa

    Observed band size: 65 kDa, 75 kDa, 85 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] (ab280885), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] (ab280885)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labelling Protein S with ab280885 at 1/1000 dilution (1.603 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the leydig cells in human testis (PMID: 7945211). The section was incubated with ab280885 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Protein S antibody [EPR24509-70] (ab280885), expandable thumbnail

    Western blot - Anti-Protein S antibody [EPR24509-70] (ab280885)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:28118606, 27447084).

    Exposure time: 15 seconds

    All lanes: Western blot - Anti-Protein S antibody [EPR24509-70] (ab280885) at 1/2000 dilution

    All lanes: Human plasma at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 75 kDa

    Observed band size: 75 kDa, 85 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] (ab280885), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] (ab280885)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Protein S with ab280885 at 1/1000 dilution (1.603 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the plasma in human colon. The section was incubated with ab280885 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] (ab280885), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] (ab280885)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling Protein S with ab280885 at 1/1000 dilution (1.603 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human pancreas (PMID:7822769). The section was incubated with ab280885 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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