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Rabbit Recombinant Monoclonal Protein S antibody. Suitable for IHC-P, WB and reacts with Mouse samples.

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Images

Western blot - Anti-Protein S antibody [EPR28196-36] (AB316281), expandable thumbnail
  • Western blot - Anti-Protein S antibody [EPR28196-36] (AB316281), expandable thumbnail
  • Western blot - Anti-Protein S antibody [EPR28196-36] (AB316281), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] (AB316281), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] (AB316281), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IFIHC-PIPWB
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.

Alternative names

Pros, Stil, Sil, Vitamin K-dependent protein S

Recommended products

Rabbit Recombinant Monoclonal Protein S antibody. Suitable for IHC-P, WB and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28196-36
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Protein S also known as PROS1 is a vitamin K-dependent plasma glycoprotein with a molecular mass of approximately 75 kDa. It is synthesized mainly in the liver but also found in other tissues such as the endothelium megakaryocytes and osteoblasts. This protein plays a significant role in the regulation of blood coagulation by serving as a non-enzymatic cofactor for activated protein C (APC) in the proteolytic degradation of coagulation factors Va and VIIIa.

Biological function summary

The integrated function of Protein S within the coagulation cascade cannot be overemphasized. It acts synergistically with APC to limit thrombin generation therefore facilitating anticoagulation. Protein S is part of a complex with APC that exerts a potent anticoagulant effect. While free Protein S shows functional activity a large fraction circulates in complex with C4b-binding protein where it exhibits limited cofactor activity.

Pathways

The anticoagulant role of Protein S anchors it within the critical pathways like the protein C pathway impacting hemostasis. It directly associates with proteins such as Factor Va and Factor VIIIa and indirectly with proteins like thrombin. The regulation of coagulation through these pathways highlights its contribution to maintaining vascular integrity and preventing thrombosis.

Associated diseases and disorders

Protein S deficiency has clinical implications in thrombotic conditions including deep vein thrombosis (DVT) and pulmonary embolism (PE). Such deficiencies can be hereditary or acquired often linked to disorders affecting vitamin K metabolism or liver function. Protein C another key anticoagulant closely interacts with Protein S in these disorders highlighting their collaborative effort in preventing excessive clot formation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Protein S antibody [EPR28196-36] (ab316281), expandable thumbnail

    Western blot - Anti-Protein S antibody [EPR28196-36] (ab316281)

    Low expression: cerebellum (PMID: 7822769)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane 1: 48s, Lane 2-3: 136s, Lane 4: 125 seconds

    All lanes: Western blot - Anti-Protein S antibody [EPR28196-36] (ab316281) at 1/1000 dilution

    Lane 1: Mouse serum lysate at 50 µg with 5% NFDM/TBST

    Lane 2: Mouse spleen tissue lysate at 50 µg with 5% NFDM/TBST

    Lane 3: Mouse cerebellum tissue lysate at 50 µg with 5% NFDM/TBST

    Lane 4: Mouse plasma lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 80 kDa, 36 kDa

  • Western blot - Anti-Protein S antibody [EPR28196-36] (ab316281), expandable thumbnail

    Western blot - Anti-Protein S antibody [EPR28196-36] (ab316281)

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-Protein S antibody [EPR28196-36] (ab316281) at 1/1000 dilution

    Lane 1: Mouse testis tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 80 kDa

    Exposure time: 125s

  • Western blot - Anti-Protein S antibody [EPR28196-36] (ab316281), expandable thumbnail

    Western blot - Anti-Protein S antibody [EPR28196-36] (ab316281)

    Low expression: EL4.

    The identity of the higher MW band at approximately 300 kDa (in lane 1 and 2) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Protein S antibody [EPR28196-36] (ab316281) at 1/1000 dilution

    Lane 1: 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 50 µg with 5% NFDM/TBST

    Lane 2: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 50 µg with 5% NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 80 kDa, 36 kDa

    Exposure time: 136s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] (ab316281), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] (ab316281)

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Protein S with ab316281 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in mouse pancreas.

    The section was incubated with ab316281 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] (ab316281), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] (ab316281)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Protein S with ab316281 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: No staining in mouse cerebrum.

    The section was incubated with ab316281 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] (ab316281), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] (ab316281)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Protein S with ab316281 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on megakaryocytes and platelets in mouse spleen (PMID: 32276277).

    The section was incubated with ab316281 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] (ab316281), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] (ab316281)

    Immunohistochemical analysis of paraffin-embedded Mouse placenta tissue labeling Protein S with ab316281 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in plasma of mouse placenta.

    The section was incubated with ab316281 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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