Anti-Protein S antibody [EPR28196-36] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] - BSA and Azide free (AB316282)

This data was developed using ab316281, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Protein S with ab316281 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining in mouse cerebrum.
The section was incubated with ab316281 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] - BSA and Azide free (AB316282)

This data was developed using ab316281, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Protein S with ab316281 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse pancreas.
The section was incubated with ab316281 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] - BSA and Azide free (AB316282)

This data was developed using ab316281, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse placenta tissue labeling Protein S with ab316281 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in plasma of mouse placenta.
The section was incubated with ab316281 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR28196-36] - BSA and Azide free (AB316282)

This data was developed using ab316281, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Protein S with ab316281 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on megakaryocytes and platelets in mouse spleen (PMID : 32276277).
The section was incubated with ab316281 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Protein S antibody [EPR28196-36] - BSA and Azide free (AB316282)

This data was developed using ab316281, the same antibody clone in a different buffer formulation.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-Protein S antibody [EPR28196-36] (<a href='/en-us/products/primary-antibodies/protein-s-antibody-epr28196-36-ab316281'>ab316281</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg with 5% NFDM/TBST

Lane 2:

Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

false

Exposure time: 125s

• WB

Supplier Data

Western blot - Anti-Protein S antibody [EPR28196-36] - BSA and Azide free (AB316282)

This data was developed using ab316281, the same antibody clone in a different buffer formulation.

Low expression : EL4.

The identity of the higher MW band at approximately 300 kDa (in lane 1 and 2) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Protein S antibody [EPR28196-36] (<a href='/en-us/products/primary-antibodies/protein-s-antibody-epr28196-36-ab316281'>ab316281</a>) at 1/1000 dilution

Lane 1:

3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 50 µg with 5% NFDM/TBST

Lane 2:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 50 µg with 5% NFDM/TBST

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 136s

• WB

Supplier Data

Western blot - Anti-Protein S antibody [EPR28196-36] - BSA and Azide free (AB316282)

This data was developed using ab316281, the same antibody clone in a different buffer formulation.

Low expression : cerebellum (PMID : 7822769)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 48s, Lane 2-3 : 136s, Lane 4 : 125 seconds

All lanes:

Western blot - Anti-Protein S antibody [EPR28196-36] (<a href='/en-us/products/primary-antibodies/protein-s-antibody-epr28196-36-ab316281'>ab316281</a>) at 1/1000 dilution

Lane 1:

Mouse serum lysate at 50 µg with 5% NFDM/TBST

Lane 2:

Mouse spleen tissue lysate at 50 µg with 5% NFDM/TBST

Lane 3:

Mouse cerebellum tissue lysate at 50 µg with 5% NFDM/TBST

Lane 4:

Mouse plasma lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 80 kDa,36 kDa

false