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AB286163

Anti-Proteinase K antibody

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Rabbit Polyclonal Proteinase K antibody. Suitable for WB and reacts with Recombinant full length protein - Parengyodontium album samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Parengyodontium album Proteinase K.

View Alternative Names

Proteinase K, Endopeptidase K, Tritirachium alkaline proteinase, PROK

1 Images
Western blot - Anti-Proteinase K antibody (AB286163)
  • WB

Supplier Data

Western blot - Anti-Proteinase K antibody (AB286163)

All lanes:

Western blot - Anti-Proteinase K antibody (ab286163) at 6 µg/mL

All lanes:

Recombinant Proteinase K

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Parengyodontium album

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Parengyodontium album Proteinase K.

P06873

Specificity

Expected to react with Tritirachium album Proteinase K

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Parengyodontium album": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Parengyodontium album": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "6 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 6210 Proteinase K Antibody. 6210-100 is the same size as the 100 µg size of ab286163.
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 Preservative: 0.03% Proclin 300 Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteinase K also known by alternate names like pronase dako proteinase K prokeinase K protein K and protease K is a serine protease widely used in molecular biology. This enzyme derived from the fungus Engyodontium album has a molecular mass of approximately 28.9 kDa. Proteinase K demonstrates high proteolytic activity effectively breaking down proteins by cleaving peptide bonds adjacent to the carboxyl group of aliphatic and aromatic amino acids. Researchers often utilize this enzyme in DNA and RNA purification protocols due to its ability to inactivate nucleases. It is typically expressed in microbial or cell-free systems optimized for high yield and activity.
Biological function summary

Proteinase K plays a critical role in protein degradation. It exhibits robust activity even in the presence of detergents and high temperatures characteristics that make it a powerful tool in the removal of proteins during nucleic acid isolation. This enzyme is not a part of any complex within the organism but acts independently in protein digestion processes. Its ability to function under extreme conditions expands its utility across various biological applications.

Pathways

Proteinase K engages in pathways related to protein catabolism and turnover. It does not contribute directly to metabolic pathways within living organisms; however it assists experimental pathways in vitro by efficiently degrading proteins. Proteinase K's proteolytic capabilities make it instrumental in preventing protein contamination aiding the study of valid biological interactions without extraneous protein interference. Related proteins like pepsin and trypsin also participate in protein degradation but they have different activity profiles and optimal conditions.

Proteinase K is frequently employed in research on genetic disorders and infectious diseases. While the enzyme itself is not associated with causing diseases its role in DNA and RNA extraction is vital for diagnosing conditions such as hereditary diseases and viral infections. Through these processes Proteinase K indirectly interacts with proteins relevant to diseases like cystic fibrosis and HIV. By facilitating the isolation of pure nucleic acids Proteinase K supports studies aiming to elucidate the genetic and viral proteins connected with these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes keratin at aromatic and hydrophobic residues.
See full target information Proteinase K
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Product promise

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