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AB325745

Anti-Proteinase K antibody [EPR29774-70]

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Rabbit Recombinant Monoclonal Proteinase K antibody. Suitable for WB and reacts with Recombinant fragment - Parengyodontium album samples.

View Alternative Names

Proteinase K, Endopeptidase K, Tritirachium alkaline proteinase, PROK

2 Images
Western blot - Anti-Proteinase K antibody [EPR29774-70] (AB325745)
  • WB

Lab

Western blot - Anti-Proteinase K antibody [EPR29774-70] (AB325745)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with Species independent Proteinase R.

In Western blot, Anti-His tag® antibody (ab15149) (1 : 1000)(37KDa).

All lanes:

Western blot - Anti-Proteinase K antibody [EPR29774-70] (ab325745) at 1/1000 dilution

Lane 1:

His-tagged Species independent Proteinase K recombinant fragment at 1 ng

Lane 2:

His-tagged Species independent Proteinase R recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa

false

Exposure time: 15s

Western blot - Anti-Proteinase K antibody [EPR29774-70] (AB325745)
  • WB

Lab

Western blot - Anti-Proteinase K antibody [EPR29774-70] (AB325745)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Proteinase K antibody [EPR29774-70] (ab325745) at 1/1000 dilution

All lanes:

Commercially available Proteinase K fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 29 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29774-70

Isotype

IgG

Carrier free

No

Reacts with

Parengyodontium album

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Parengyodontium album": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Parengyodontium album": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes keratin at aromatic and hydrophobic residues.
See full target information Proteinase K
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com