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AB8469

Anti-Proteus spp antibody [31-17]

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(1 Publication)

Mouse Monoclonal Proteus spp antibody. Suitable for ICC/IF and reacts with Proteus samples. Cited in 1 publication.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

31-17

Isotype

IgG2a

Light chain type

unknown

Carrier free

No

Reacts with

Proteus

Applications

ICC/IF

applications

Specificity

This antibody reacts with approximately 50% of the Proteus spp. isolates tested

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Proteus": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Detection by luminescence, polymerase chain reaction or culture. Magnisort-M particles (DuPont) are diluted 25 times in PBS (+ 1% gelatin). 50 μl is transferred into a microtiterplate well. Add 50-75 μl antibody solution, incubate shaking 15 min. at room temperature. Magnetize plate and remove supernatant. Add 75 μl bacteria suspension and incubate shaking 15 min. at room temperature. Magnetize plate and wash beads three times with PBS-gelatin. Resuspend beads in appropriate volume and prepare for specific detection.</p>" } } }

Product details

This antibody reacts with bacteria in suspension and is therefore useful for detection of Proteus spp. in bacterial suspensions. Various systems can be used with this antibody to detect the presence of Proteus spp.

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
This product is sterile filtered.
Storage buffer
pH: 7.3 Preservative: 0.1% Sodium azide Constituents: 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteus spp. is a gram-negative bacterium that possesses versatile mechanical capabilities. Known for its flagellar motility Proteus spp. thrive in various environments typically in soil water and the intestinal tracts of humans and animals. This bacterium exhibits a unique swarming ability which is a coordinated movement that differentiates it from other bacteria. At a mechanical level its distinct ability to form crystalline-like biofilms on surfaces contributes to its persistence and survival. While the mass of individual components varies key structures like the flagella are essential for its motility and surface adherence.
Biological function summary

Proteus spp. participate in several functions critical for survival and adaptability. As an opportunistic pathogen it can cause infections when the host's defense is compromised. Its role in urea hydrolysis by producing urease is significant; this enzyme catalyzes the conversion of urea into ammonia and carbon dioxide increasing local pH. Proteus spp. do not form a complex with other proteins but their urease production impacts their environment.

Pathways

Proteus spp. influence nitrogen metabolism and biofilm formation pathways. Nitrogen metabolism plays a part in nutrient cycling particularly when Proteus breaks down urea. In biofilm formation it interacts with other proteins like quorum-sensing molecules that regulate gene expression related to community behavior. These processes are important for the adaptation of Proteus in various habitats.

Proteus spp. often associate with urinary tract infections and kidney stone development. The association with urinary tract infections arises from their motile nature enabling colonization of the urinary system. Urease the enzyme produced by Proteus plays a role in kidney stone formation by increasing ammonia levels which elevates pH and leads to crystal formation. These conditions highlight the importance of Proteus spp. in clinical settings underlining the need for efficient medical interventions to manage infections and complications.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes 15: PubMed38927720

2024

Genetic Analysis of the Plasmid-Based Temperature-Lethal Mutant in .

Applications

Unspecified application

Species

Unspecified reactive species

Haoyang Zhang,Zhili Yang,Jianhua Liu
View all publications

Product promise

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