AB184015

Anti-Prothrombin antibody

Name: Anti-Prothrombin antibody (ab184015) | Abcam
Brand: Abcam Limited
SKU: AB184015

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(1 Publication)

Rabbit Polyclonal Thrombin antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human F2 aa 1-250.

View Alternative Names

Prothrombin, Coagulation factor II, F2

3 Images

Supplier Data

Western blot - Anti-Prothrombin antibody (AB184015)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Prothrombin antibody (ab184015) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Predicted band size: 70 kDa

false

Supplier Data

Western blot - Anti-Prothrombin antibody (AB184015)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-Prothrombin antibody (ab184015) at 1/500 dilution

All lanes:

Human plasma at 30 µg

Predicted band size: 70 kDa

false

Supplier Data

Western blot - Anti-Prothrombin antibody (AB184015)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-Prothrombin antibody (ab184015) at 1/500 dilution

All lanes:

Mouse plasma at 50 µg

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

applications

Immunogen

Recombinant Fragment Protein within Human F2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P00734

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prothrombin also known as coagulation factor II is an important protein in blood clotting with an approximate mass of 72 kDa. This protein is synthesized in the liver and is expressed mainly in the plasma as an inactive precursor of thrombin. Prothrombin plays a pivotal role in the conversion into active thrombin through factor X activation in the presence of ionized calcium and phospholipid surfaces. Thrombin then further acts to convert fibrinogen into fibrin leading to clot formation. Prothrombin also can interact with various cellular receptors influencing other pathways beyond coagulation.

Biological function summary

Prothrombin regulates hemostasis and is part of the prothrombinase complex along with factor Xa factor Va calcium ions and phospholipids. This complex allows for the precise regulation of thrombin generation on the surface of activated platelets. By controlling the formation of thrombin prothrombin affects not only blood coagulation but also influences cell proliferation inflammation and tissue repair. The careful balance of prothrombin activation is important to prevent excessive bleeding or clot formation.

Pathways

Prothrombin is an essential component of the coagulation cascade especially the intrinsic and extrinsic coagulation pathways. In these pathways it links with proteins such as fibrinogen and factor X contributing to the amplification and perpetuation of thrombin generation. Additionally thrombin produced from prothrombin engages in a feedback loop to further enhance factor V VIII and XI activation. Through these actions prothrombin helps maintain normal coagulation but must be tightly regulated to prevent thrombotic complications.

Prothrombin is implicated in conditions like prothrombin thrombophilia and liver disease. Prothrombin thrombophilia is a genetic disorder that increases the risk for venous thromboembolism due to the presence of the G20210A mutation in the prothrombin gene. Moreover impaired prothrombin synthesis can occur as a result of liver disease leading to a higher bleeding risk. In thrombotic conditions proteins like protein C and antithrombin work to combat excessive prothrombin-generated thrombin underlining the necessity for balance and regulation in hemostatic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces (PubMed : 2019570, PubMed : 21976677). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells (PubMed : 30568593, PubMed : 9780208).

See full target information F2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2019:6507537 PubMed31354911

2019

HDAC6 Inhibition Protects against OGDR-Induced Golgi Fragmentation and Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Jieqiong Tan,Zhiping Hu,Chunli Chen,Liuwang Zeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prothrombin antibody

Western blot - Anti-Prothrombin antibody (AB184015)

Western blot - Anti-Prothrombin antibody (AB184015)

Western blot - Anti-Prothrombin antibody (AB184015)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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