AB251497

Anti-Prothrombin antibody [EPR20131] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Thrombin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

Cf2, F2, Prothrombin, Coagulation factor II

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (AB251497)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (AB251497)

This data was developed using ab208590, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Prothrombin with ab208590 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat liver was observed (PMID : 1705822). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (AB251497)

This data was developed using ab208590, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Prothrombin with ab208590 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse liver was observed (PMID : 1705822). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (AB251497)

This data was developed using ab208590, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Prothrombin with ab208590 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on plasma and endothelial cells of mouse kidney was observed (PMID : 16410745). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (AB251497)

This data was developed using ab208590, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Prothrombin is proteolytically cleaved into different fragments. The fragment profile is consistent with the literature (PMID : 17637839; 21131592; 16734589).

All lanes:

Western blot - Anti-Prothrombin antibody [EPR20131] (<a href='/en-us/products/primary-antibodies/prothrombin-antibody-epr20131-ab208590'>ab208590</a>) at 1/1000 dilution

Lane 1:

Mouse plasma at 20 µg

Lane 2:

Rat plasma at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 70 kDa

Observed band size: 33 kDa,48 kDa,72 kDa

true

Exposure time: 15s

Unknown

Western blot - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (AB251497)

This data was developed using ab208590, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Prothrombin antibody [EPR20131] (<a href='/en-us/products/primary-antibodies/prothrombin-antibody-epr20131-ab208590'>ab208590</a>) at 1/1000 dilution

Lane 1:

Mouse liver lysate at 20 µg

Lane 2:

Rat liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 70 kDa

Observed band size: 72 kDa

true

Exposure time: 10s

Unconjugated

Anti-Prothrombin antibody [EPR20131]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20131

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab251497 is the carrier-free version of ab208590.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prothrombin also known as coagulation factor II is an important protein in blood clotting with an approximate mass of 72 kDa. This protein is synthesized in the liver and is expressed mainly in the plasma as an inactive precursor of thrombin. Prothrombin plays a pivotal role in the conversion into active thrombin through factor X activation in the presence of ionized calcium and phospholipid surfaces. Thrombin then further acts to convert fibrinogen into fibrin leading to clot formation. Prothrombin also can interact with various cellular receptors influencing other pathways beyond coagulation.

Biological function summary

Prothrombin regulates hemostasis and is part of the prothrombinase complex along with factor Xa factor Va calcium ions and phospholipids. This complex allows for the precise regulation of thrombin generation on the surface of activated platelets. By controlling the formation of thrombin prothrombin affects not only blood coagulation but also influences cell proliferation inflammation and tissue repair. The careful balance of prothrombin activation is important to prevent excessive bleeding or clot formation.

Pathways

Prothrombin is an essential component of the coagulation cascade especially the intrinsic and extrinsic coagulation pathways. In these pathways it links with proteins such as fibrinogen and factor X contributing to the amplification and perpetuation of thrombin generation. Additionally thrombin produced from prothrombin engages in a feedback loop to further enhance factor V VIII and XI activation. Through these actions prothrombin helps maintain normal coagulation but must be tightly regulated to prevent thrombotic complications.

Prothrombin is implicated in conditions like prothrombin thrombophilia and liver disease. Prothrombin thrombophilia is a genetic disorder that increases the risk for venous thromboembolism due to the presence of the G20210A mutation in the prothrombin gene. Moreover impaired prothrombin synthesis can occur as a result of liver disease leading to a higher bleeding risk. In thrombotic conditions proteins like protein C and antithrombin work to combat excessive prothrombin-generated thrombin underlining the necessity for balance and regulation in hemostatic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity). Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces (By similarity). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells (By similarity).

See full target information F2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prothrombin antibody [EPR20131] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (AB251497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (AB251497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (AB251497)

Western blot - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (AB251497)

Western blot - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (AB251497)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise