AB233297

Anti-Prothrombin fragment 2 antibody

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(1 Publication)

Rabbit Polyclonal Thrombin antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Rat, Pig samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human F2 aa 1-350.

View Alternative Names

Prothrombin, Coagulation factor II, F2

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (AB233297)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (AB233297)

Formalin-fixed, paraffin-embedded human skin cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (AB233297)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (AB233297)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (AB233297)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (AB233297)

Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (AB233297)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (AB233297)

Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (AB233297)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (AB233297)

Formalin-fixed, paraffin-embedded human bile duct cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Western blot - Anti-Prothrombin fragment 2 antibody (AB233297)

WB

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Western blot - Anti-Prothrombin fragment 2 antibody (AB233297)

All lanes:

Western blot - Anti-Prothrombin fragment 2 antibody (ab233297) at 2 µg/mL

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

Pig kidney tissue lysate

Lane 3:

Rat serum

Secondary

All lanes:

HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution

false

Western blot - Anti-Prothrombin fragment 2 antibody (AB233297)

WB

Supplier Data

Western blot - Anti-Prothrombin fragment 2 antibody (AB233297)

All lanes:

Western blot - Anti-Prothrombin fragment 2 antibody (ab233297) at 2 µg/mL

All lanes:

Recombinant human Prothrombin fragment 2 protein

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Pig

Applications

IHC-P, WB

Immunogen

Recombinant Fragment Protein within Human F2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prothrombin fragment 2 also known as F2 fragment is a part of the prothrombin protein that is released during the activation of prothrombin to thrombin. It has a molecular mass of approximately 28 kDa. This fragment appears in the blood plasma specifically produced by the liver cells. In clinical settings the presence of prothrombin fragment 2 can be measured as an indicator of coagulation activity.

Biological function summary

Prothrombin fragment 2 plays a significant role in the blood coagulation process. It is a result of cleavage from prothrombin a glycoprotein that circulates in the plasma. It acts as an important marker for the generation of thrombin which is an active enzyme necessary for fibrin clot formation. The release of the fragment happens during the transformation of prothrombin to thrombin by the prothrombinase complex which includes factor Xa and factor Va.

Pathways

Prothrombin fragment 2 is involved in the coagulation cascade specifically in the common pathway. This pathway is essential for the formation of cross-linked fibrin forming the stable structure of a blood clot. Related proteins include factor Xa which helps activate prothrombin into thrombin and factor Va which acts as a cofactor in the prothrombinase complex highlighting the interconnected nature of the clotting mechanism.

Prothrombin fragment 2 has a correlation with thrombotic disorders and cardiovascular diseases. Elevated levels of this fragment serve as a biomarker for conditions like deep vein thrombosis and atherosclerosis indicating increased thrombin production. Factor Xa another player in the clotting pathway is often targeted in therapeutic interventions for these conditions illustrating the critical nature of understanding the role of prothrombin fragment 2 in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces (PubMed : 2019570, PubMed : 21976677). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells (PubMed : 30568593, PubMed : 9780208).

See full target information F2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0310389 PubMed39292698

2024

Visfatin (NAMPT) expression in human placenta cells in normal and pathological conditions and its hormonal regulation in trophoblast JEG-3 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Dawid,Patrycja Kurowska,Piotr Pawlicki,Małgorzata Kotula-Balak,Tomasz Milewicz,Joelle Dupont,Agnieszka Rak

View all publications

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Product promise

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