Rabbit Polyclonal Thrombin antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Rat, Pig samples. Immunogen corresponding to Recombinant Fragment Protein within Human F2 aa 1-350.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human F2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link P00734
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Tested | Expected |
| Orangutan | Predicted | Predicted |
| Pig | Tested | Expected |
| Recombinant full length protein - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
Species Recombinant full length protein - Human | Dilution info 0.5-2 µg/mL | Notes - |
Species Rat | Dilution info 0.5-2 µg/mL | Notes - |
Species Pig | Dilution info 0.5-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Function
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces (PubMed:2019570, PubMed:21976677). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells (PubMed:30568593, PubMed:9780208).
Alternative names
Prothrombin, Coagulation factor II, F2
Recommended products
Rabbit Polyclonal Thrombin antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Rat, Pig samples. Immunogen corresponding to Recombinant Fragment Protein within Human F2 aa 1-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human F2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link P00734
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Prothrombin fragment 2 also known as F2 fragment is a part of the prothrombin protein that is released during the activation of prothrombin to thrombin. It has a molecular mass of approximately 28 kDa. This fragment appears in the blood plasma specifically produced by the liver cells. In clinical settings the presence of prothrombin fragment 2 can be measured as an indicator of coagulation activity.
- Biological function summary
Prothrombin fragment 2 plays a significant role in the blood coagulation process. It is a result of cleavage from prothrombin a glycoprotein that circulates in the plasma. It acts as an important marker for the generation of thrombin which is an active enzyme necessary for fibrin clot formation. The release of the fragment happens during the transformation of prothrombin to thrombin by the prothrombinase complex which includes factor Xa and factor Va.
- Pathways
Prothrombin fragment 2 is involved in the coagulation cascade specifically in the common pathway. This pathway is essential for the formation of cross-linked fibrin forming the stable structure of a blood clot. Related proteins include factor Xa which helps activate prothrombin into thrombin and factor Va which acts as a cofactor in the prothrombinase complex highlighting the interconnected nature of the clotting mechanism.
- Associated diseases and disorders
Prothrombin fragment 2 has a correlation with thrombotic disorders and cardiovascular diseases. Elevated levels of this fragment serve as a biomarker for conditions like deep vein thrombosis and atherosclerosis indicating increased thrombin production. Factor Xa another player in the clotting pathway is often targeted in therapeutic interventions for these conditions illustrating the critical nature of understanding the role of prothrombin fragment 2 in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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7 product images
Western blot - Anti-Prothrombin fragment 2 antibody (ab233297)
All lanes: Western blot - Anti-Prothrombin fragment 2 antibody (ab233297) at 2 µg/mL
Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2: Pig kidney tissue lysate
Lane 3: Rat serum
SecondaryAll lanes: HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (ab233297)
Formalin-fixed, paraffin-embedded human skin cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (ab233297)
Formalin-fixed, paraffin-embedded human bile duct cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (ab233297)
Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (ab233297)
Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin fragment 2 antibody (ab233297)
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Prothrombin fragment 2 with ab233297 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Western blot - Anti-Prothrombin fragment 2 antibody (ab233297)
All lanes: Western blot - Anti-Prothrombin fragment 2 antibody (ab233297) at 2 µg/mL
All lanes: Recombinant human Prothrombin fragment 2 protein
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