AB33219

Anti-PROX1 antibody [5G10]

Name: Anti-PROX1 antibody [5G10] (ab33219) | Abcam
Brand: Abcam Limited
SKU: AB33219
Price: 195 USD
Availability: InStock
Rating: 3 (5 reviews)

(5 Reviews)

(6 Publications)

Mouse Monoclonal PROX1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Prospero homeobox protein 1, Homeobox prospero-like protein PROX1, PROX-1, PROX1

2 Images

Project

Western blot - Anti-PROX1 antibody [5G10] (AB33219)

All lanes:

Western blot - Anti-PROX1 antibody [5G10] (ab33219) at 10 µg/mL

Lane 1:

Western blot - Hep G2 nuclear extract lysate (<a href='/en-us/products/cell-lysates/hep-g2-nuclear-extract-lysate-ab14660'>ab14660</a>) at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (negative control) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

true

Exposure time: 20min

Collaborator

Western blot - Anti-PROX1 antibody [5G10] (AB33219)

All lanes:

Western blot - Anti-PROX1 antibody [5G10] (ab33219) at 10 µg/mL

All lanes:

P3 Dentate gyrus whole tissue lysate at 50 µg

Predicted band size: 83 kDa

Observed band size: 105 kDa

true

Exposure time: 20min

Image courtesy of an Abreview submitted by Dr. Xia Chen

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5G10

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PROX1 protein also known as Prospero homeobox 1 acts mechanically as a transcription factor and plays an important role in cell differentiation. Its molecular mass is about 83 kilodaltons. This protein is expressed in a range of tissues including the liver heart and lymphatic system. During embryonic development PROX1 is notably active where it participates in organogenesis and differentiation into specific cell types.

Biological function summary

PROX1 influences numerous processes by regulating gene expression. It forms part of several protein complexes that determine the fate of lymphatic endothelial cells balancing between lymphatic and blood vascular destinies. PROX1 directly affects the promotion and maintenance of the lymphatic endothelial cell phenotype. Its activity is fundamental to the development and maintenance of several organs and tissues impacting overall physiological function.

Pathways

The transcription factor PROX1 integrates into the VEGF signaling pathway and the Wnt signaling pathway. Within these pathways it interacts closely with other proteins such as VEGF-C and β-catenin. PROX1 modulates these pathways to orchestrate cell proliferation migration and vessel formation. Through its interactions PROX1 significantly contributes to the intricate communication between cells in developing tissues.

Research indicates that dysregulation of PROX1 is linked to cancer and lymphatic diseases. In cancer particularly colorectal and breast cancers aberrant PROX1 expression can promote tumor progression. It interacts with proteins like NF-κB which influence inflammatory responses and cell survival. Similarly in lymphedema altered PROX1 can disrupt normal lymphatic development affecting fluid balance and immune function in the body.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses : transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components : BMAL1, NPAS2 and CRY1 and metabolic genes : AVPR1A and ELOVL3.

See full target information PROX1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of experimental pathology 102:268-278 PubMed34791715

2021

Immunohistochemical and immunofluorescence expression profile of lymphatic endothelial cell markers in oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kullasit Chutipongpisit,V Praveen Parachuru,Lara T Friedlander,Haizal M Hussaini,Alison M Rich

Nature communications 10:5220 PubMed31745086

2019

A morphogenetic EphB/EphrinB code controls hepatopancreatic duct formation.

Applications

Unspecified application

Species

Unspecified reactive species

M Ilcim Thestrup,Sara Caviglia,Jordi Cayuso,Ronja L S Heyne,Racha Ahmad,Wolfgang Hofmeister,Letizia Satriano,David G Wilkinson,Jesper B Andersen,Elke A Ober

Veterinary research 50:22 PubMed30894203

2019

Nuclear localization of Newcastle disease virus matrix protein promotes virus replication by affecting viral RNA synthesis and transcription and inhibiting host cell transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Duan,Shanshan Deng,Xinqin Ji,Jiafu Zhao,Chao Yuan,Hongbo Gao

Scientific reports 7:2521 PubMed28566680

2017

Dibutyl phthalate induced testicular dysgenesis originates after seminiferous cord formation in rats.

Applications

Species

Rat

Nathália L M Lara,Sander van den Driesche,Sheila Macpherson,Luiz R França,Richard M Sharpe

Cells, tissues, organs 201:268-76 PubMed27144771

2016

Identifying Behavioral Phenotypes and Heterogeneity in Heart Valve Surface Endothelium.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia A Blancas,Liezl R Balaoing,Francisca M Acosta,K Jane Grande-Allen

Hybridoma (2005) 25:27-33 PubMed16475879

2006

Production of monoclonal antibodies against Prox1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoren Chen,Tapan P Patel,William J Cain,Melinda K Duncan

View all publications

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Product promise

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