AB38692

Anti-PROX1 antibody

(8 Reviews)

(16 Publications)

Rabbit Polyclonal PROX1 antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications.

View Alternative Names

Prospero homeobox protein 1, Homeobox prospero-like protein PROX1, PROX-1, PROX1

1 Images

Lab

Western blot - Anti-PROX1 antibody (AB38692)

Gel type : MOPS

Blocking buffer : 3% milk

All lanes:

Western blot - Anti-PROX1 antibody (ab38692) at 1 µg/mL

Lane 1:

HepG2 whole cell lysate at 10 µg

Lane 2:

SHSY5Y whole cell lysate at 10 µg

Lane 3:

Hek293 whole cell lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 83 kDa

Observed band size: 95 kDa

false

Exposure time: 12min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

We have had mixed results for use of this antibody in IHC-P. The AbReview below from an external researcher reports good staining with this antibody with human heart tissue, but we have had mixed results with this antibody in internal QC testing in IHC-P. We welcome any feedback from customers who have used this antibody in IHC-P.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PROX1 protein also known as Prospero homeobox 1 acts mechanically as a transcription factor and plays an important role in cell differentiation. Its molecular mass is about 83 kilodaltons. This protein is expressed in a range of tissues including the liver heart and lymphatic system. During embryonic development PROX1 is notably active where it participates in organogenesis and differentiation into specific cell types.

Biological function summary

PROX1 influences numerous processes by regulating gene expression. It forms part of several protein complexes that determine the fate of lymphatic endothelial cells balancing between lymphatic and blood vascular destinies. PROX1 directly affects the promotion and maintenance of the lymphatic endothelial cell phenotype. Its activity is fundamental to the development and maintenance of several organs and tissues impacting overall physiological function.

Pathways

The transcription factor PROX1 integrates into the VEGF signaling pathway and the Wnt signaling pathway. Within these pathways it interacts closely with other proteins such as VEGF-C and β-catenin. PROX1 modulates these pathways to orchestrate cell proliferation migration and vessel formation. Through its interactions PROX1 significantly contributes to the intricate communication between cells in developing tissues.

Research indicates that dysregulation of PROX1 is linked to cancer and lymphatic diseases. In cancer particularly colorectal and breast cancers aberrant PROX1 expression can promote tumor progression. It interacts with proteins like NF-κB which influence inflammatory responses and cell survival. Similarly in lymphedema altered PROX1 can disrupt normal lymphatic development affecting fluid balance and immune function in the body.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses : transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components : BMAL1, NPAS2 and CRY1 and metabolic genes : AVPR1A and ELOVL3.

See full target information PROX1

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Microcirculation (New York, N.Y. : 1994) 30:e12788 PubMed36169611

2022

Impact of Goreisan components on rat mesenteric collecting lymphatic vessel pumping.

Applications

Unspecified application

Species

Unspecified reactive species

Michiko Jo,Andrea N Trujillo,Naotoshi Shibahara,Jerome W Breslin

Cell reports 39:110667 PubMed35417707

2022

Dynamic interplay between thalamic activity and Cajal-Retzius cells regulates the wiring of cortical layer 1.

Applications

Unspecified application

Species

Unspecified reactive species

Ioana Genescu,Mar Aníbal-Martínez,Vladimir Kouskoff,Nicolas Chenouard,Caroline Mailhes-Hamon,Hugues Cartonnet,Ludmilla Lokmane,Filippo M Rijli,Guillermina López-Bendito,Frédéric Gambino,Sonia Garel

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22248 PubMed35239213

2022

Tumor microenvironment remodeling modulates macrophage phenotype in breast cancer lymphangiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Du,Manlin Cao,Yiwen Liu,Yiqing He,Cuixia Yang,Guoliang Zhang,Youben Fan,Feng Gao

Nature protocols 16:919-936 PubMed33432231

2021

Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroyuki Koike,Kentaro Iwasawa,Rie Ouchi,Mari Maezawa,Masaki Kimura,Asuka Kodaka,Shozo Nishii,Wendy L Thompson,Takanori Takebe

Current biology : CB 30:3507-3521.e7 PubMed32707059

2020

eEF2/eEF2K Pathway in the Mature Dentate Gyrus Determines Neurogenesis Level and Cognition.

Applications

Unspecified application

Species

Unspecified reactive species

Elham Taha,Sudarshan Patil,Iliana Barrera,Julia Panov,Mohammad Khamaisy,Christopher G Proud,Clive R Bramham,Kobi Rosenblum

Lymphatic research and biology 18:329-332 PubMed31880509

2019

Axillary Web Syndrome: Evidence for Lymphatic Origin with Thrombosis.

Applications

Unspecified application

Species

Unspecified reactive species

Karin Johansson,Heung Chong,Cristina-Daria Ciornei,Håkan Brorson,Peter S Mortimer

Acta neuropathologica 139:383-401 PubMed31696318

2019

Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges.

Applications

Unspecified application

Species

Unspecified reactive species

Shannon Shibata-Germanos,James R Goodman,Alan Grieg,Chintan A Trivedi,Bridget C Benson,Sandrine C Foti,Ana Faro,Raphael F P Castellan,Rosa Maria Correra,Melissa Barber,Christiana Ruhrberg,Roy O Weller,Tammaryn Lashley,Jeffrey J Iliff,Thomas A Hawkins,Jason Rihel

The Journal of comparative neurology 526:742-766 PubMed29218725

2017

Characterization of retinal ganglion cell, horizontal cell, and amacrine cell types expressing the neurotrophic receptor tyrosine kinase Ret.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia Parmhans,Szilard Sajgo,Jingwen Niu,Wenqin Luo,Tudor Constantin Badea

Orphanet journal of rare diseases 11:67 PubMed27194137

2016

Pulmonary and pleural lymphatic endothelial cells from pediatric, but not adult, patients with Gorham-Stout disease and generalized lymphatic anomaly, show a high proliferation rate.

Applications

IHC

Species

Unspecified reactive species

Michiko Mori,Michael Dictor,Nicholas Brodszki,Juan Carlos López-Gutiérrez,María Beato,Jonas S Erjefält,Erik A Eklund

Methods in molecular biology (Clifton, N.J.) 1430:77-90 PubMed27172947

2016

Isolation, Identification, and Culture of Human Lymphatic Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zerina Lokmic

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PROX1 antibody

Western blot - Anti-PROX1 antibody (AB38692)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise