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AB327147

Anti-PROX1 Antibody [CL13472]

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Mouse Monoclonal PROX1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human PROX1.

View Alternative Names

Prospero homeobox protein 1, Homeobox prospero-like protein PROX1, PROX-1, PROX1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 Antibody [CL13472] (AB327147)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 Antibody [CL13472] (AB327147)

Immunohistochemical staining of human pancreas using ab327147 at 1 : 50–1 : 200 dilution, showing weak to moderate nuclear positivity in exocrine glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 Antibody [CL13472] (AB327147)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 Antibody [CL13472] (AB327147)

Immunohistochemical staining of human duodenum using ab327147 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in a subset of glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 Antibody [CL13472] (AB327147)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 Antibody [CL13472] (AB327147)

Immunohistochemical staining of rat hippocampus using ab327147 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in the granular cell layer of the dentate gyrus.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 Antibody [CL13472] (AB327147)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 Antibody [CL13472] (AB327147)

Immunohistochemical staining of mouse hippocampus using ab327147 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in the granular cell layer of the dentate gyrus.

Western blot - Anti-PROX1 Antibody [CL13472] (AB327147)
  • WB

Supplier Data

Western blot - Anti-PROX1 Antibody [CL13472] (AB327147)

Western blot analysis in human cell line SH-SY5Y using ab327147 at 1 µg/ml.

All lanes:

Western blot - Anti-PROX1 Antibody [CL13472] (ab327147)

All lanes:

Human cell line SH-SY5Y

Predicted band size: 83.2 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 Antibody [CL13472] (AB327147)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 Antibody [CL13472] (AB327147)

Immunohistochemical staining of human prostate using ab327147 at 1 : 50–1 : 200 dilution, showing no positivity in glandular cells as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 Antibody [CL13472] (AB327147)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 Antibody [CL13472] (AB327147)

Immunohistochemical staining of human skin using ab327147 at 1 : 50–1 : 200 dilution, showing no positivity in squamous epithelial cells as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL13472

Isotype

IgG1

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PROX1.

Q92786

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses : transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components : BMAL1, NPAS2 and CRY1 and metabolic genes : AVPR1A and ELOVL3.
See full target information PROX1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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