Rabbit Recombinant Monoclonal PROX1 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (AB236026), expandable thumbnail](https://content.abcam.com/products/images/prox1-antibody-epr19273-bsa-and-azide-free-ab236026--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6937.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (AB236026)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (AB236026), expandable thumbnail](https://content.abcam.com/products/images/prox1-antibody-epr19273-bsa-and-azide-free-ab236026--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130036.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (AB236026)")
![Immunocytochemistry/ Immunofluorescence - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (AB236026), expandable thumbnail](https://content.abcam.com/products/images/prox1-antibody-epr19273-bsa-and-azide-free-ab236026--immunocytochemistry-immunofluorescence-img105721.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (AB236026)")
![ChIC/CUT&RUN sequencing - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (AB236026), expandable thumbnail](https://content.abcam.com/products/images/prox1-antibody-epr19273-bsa-and-azide-free-ab236026--chic-cut-run-sequencing-img420235.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (AB236026)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIC/CUT&RUN-seq | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected |
| Mouse | Expected | Tested | Expected | Tested |
| Rat | Expected | Tested | Expected | Tested |
ChIC/CUT&RUN-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes IHC is recommended for rat and mouse only. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes IHC is recommended for rat and mouse only. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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1 product for Alternative Version
Target data
Function
Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
Alternative names
Prospero homeobox protein 1, Homeobox prospero-like protein PROX1, PROX-1, PROX1
Recommended products
Rabbit Recombinant Monoclonal PROX1 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19273
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab236026 is the carrier-free version of Anti-PROX1 antibody [EPR19273] ab199359.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PROX1 protein also known as Prospero homeobox 1 acts mechanically as a transcription factor and plays an important role in cell differentiation. Its molecular mass is about 83 kilodaltons. This protein is expressed in a range of tissues including the liver heart and lymphatic system. During embryonic development PROX1 is notably active where it participates in organogenesis and differentiation into specific cell types.
- Biological function summary
PROX1 influences numerous processes by regulating gene expression. It forms part of several protein complexes that determine the fate of lymphatic endothelial cells balancing between lymphatic and blood vascular destinies. PROX1 directly affects the promotion and maintenance of the lymphatic endothelial cell phenotype. Its activity is fundamental to the development and maintenance of several organs and tissues impacting overall physiological function.
- Pathways
The transcription factor PROX1 integrates into the VEGF signaling pathway and the Wnt signaling pathway. Within these pathways it interacts closely with other proteins such as VEGF-C and β-catenin. PROX1 modulates these pathways to orchestrate cell proliferation migration and vessel formation. Through its interactions PROX1 significantly contributes to the intricate communication between cells in developing tissues.
- Associated diseases and disorders
Research indicates that dysregulation of PROX1 is linked to cancer and lymphatic diseases. In cancer particularly colorectal and breast cancers aberrant PROX1 expression can promote tumor progression. It interacts with proteins like NF-κB which influence inflammatory responses and cell survival. Similarly in lymphedema altered PROX1 can disrupt normal lymphatic development affecting fluid balance and immune function in the body.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (ab236026), expandable thumbnail](https://content.abcam.com/products/images/prox1-antibody-epr19273-bsa-and-azide-free-ab236026--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6937.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (ab236026)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (ab236026)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling PROX1 with Anti-PROX1 antibody [EPR19273] ab199359 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nucleus staining on neurons of mouse hippocampus is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PROX1 antibody [EPR19273] ab199359).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (ab236026), expandable thumbnail](https://content.abcam.com/products/images/prox1-antibody-epr19273-bsa-and-azide-free-ab236026--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130036.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (ab236026)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (ab236026)
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling PROX1 with Anti-PROX1 antibody [EPR19273] ab199359 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nucleus staining on neurons of rat hippocampus is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PROX1 antibody [EPR19273] ab199359).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (ab236026), expandable thumbnail](https://content.abcam.com/products/images/prox1-antibody-epr19273-bsa-and-azide-free-ab236026--immunocytochemistry-immunofluorescence-img105721.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (ab236026)")
Immunocytochemistry/ Immunofluorescence - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (ab236026)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) and HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling PROX1 with Anti-PROX1 antibody [EPR19273] ab199359 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on SH-SY5Y cell line.
Negative expression in HEK-293 cells. HEK-293 doesn't express PROX1. PMID:18948102.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-PROX1 antibody [EPR19273] ab199359 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PROX1 antibody [EPR19273] ab199359).
![ChIC/CUT&RUN sequencing - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (ab236026), expandable thumbnail](https://content.abcam.com/products/images/prox1-antibody-epr19273-bsa-and-azide-free-ab236026--chic-cut-run-sequencing-img420235.jpg "ChIC/CUT&RUN sequencing - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (ab236026)")
ChIC/CUT&RUN sequencing - Anti-PROX1 antibody [EPR19273] - BSA and Azide free (ab236026)
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HepG2 (Human liver hepatocellular carcinoma cell line) cells and 5 µg of Anti-PROX1 antibody [EPR19273] ab199359 [EPR19273]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PROX1 antibody [EPR19273] ab199359).
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