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AB124910

Anti-PROX1 antibody [EPR3128(2)]

5

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal PROX1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Prospero homeobox protein 1, Homeobox prospero-like protein PROX1, PROX-1, PROX1

2 Images
Western blot - Anti-PROX1 antibody [EPR3128(2)] (AB124910)
  • WB

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Western blot - Anti-PROX1 antibody [EPR3128(2)] (AB124910)

All lanes:

Western blot - Anti-PROX1 antibody [EPR3128(2)] (ab124910) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

SHSY-5Y cell lysate at 10 µg

Predicted band size: 83 kDa

Observed band size: 95 kDa

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OI-RD Scanning - Anti-PROX1 antibody [EPR3128(2)] (AB124910)
  • OI-RD Scanning

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OI-RD Scanning - Anti-PROX1 antibody [EPR3128(2)] (AB124910)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-PROX1 antibody [EPR3128(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3128(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PROX1 protein also known as Prospero homeobox 1 acts mechanically as a transcription factor and plays an important role in cell differentiation. Its molecular mass is about 83 kilodaltons. This protein is expressed in a range of tissues including the liver heart and lymphatic system. During embryonic development PROX1 is notably active where it participates in organogenesis and differentiation into specific cell types.
Biological function summary

PROX1 influences numerous processes by regulating gene expression. It forms part of several protein complexes that determine the fate of lymphatic endothelial cells balancing between lymphatic and blood vascular destinies. PROX1 directly affects the promotion and maintenance of the lymphatic endothelial cell phenotype. Its activity is fundamental to the development and maintenance of several organs and tissues impacting overall physiological function.

Pathways

The transcription factor PROX1 integrates into the VEGF signaling pathway and the Wnt signaling pathway. Within these pathways it interacts closely with other proteins such as VEGF-C and β-catenin. PROX1 modulates these pathways to orchestrate cell proliferation migration and vessel formation. Through its interactions PROX1 significantly contributes to the intricate communication between cells in developing tissues.

Research indicates that dysregulation of PROX1 is linked to cancer and lymphatic diseases. In cancer particularly colorectal and breast cancers aberrant PROX1 expression can promote tumor progression. It interacts with proteins like NF-κB which influence inflammatory responses and cell survival. Similarly in lymphedema altered PROX1 can disrupt normal lymphatic development affecting fluid balance and immune function in the body.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses : transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components : BMAL1, NPAS2 and CRY1 and metabolic genes : AVPR1A and ELOVL3.
See full target information PROX1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 128:992-1000 PubMed25588844

2015

Front-signal-dependent accumulation of the RHOA inhibitor FAM65B at leading edges polarizes neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Gao,Wenwen Tang,Yuan Li,Pingzhao Zhang,Dejie Wang,Long Yu,Chenji Wang,Dianqing Wu
View all publications
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Product promise

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