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AB154801

Anti-PRP16 antibody [EPR9845]

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(1 Publication)

Rabbit Recombinant Monoclonal PRP16 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DDX38, KIAA0224, PRP16, DHX38, Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16, ATP-dependent RNA helicase DHX38, DEAH box protein 38

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PRP16 antibody [EPR9845] (AB154801)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PRP16 antibody [EPR9845] (AB154801)

Immunofluorescence analysis of HeLa cells labeling PRP16 with ab154801 at 1/100 dilution.

Western blot - Anti-PRP16 antibody [EPR9845] (AB154801)
  • WB

Unknown

Western blot - Anti-PRP16 antibody [EPR9845] (AB154801)

All lanes:

Western blot - Anti-PRP16 antibody [EPR9845] (ab154801) at 1/1000 dilution

Lane 1:

Raji lysate at 10 µg

Lane 2:

Jurkat lysate at 10 µg

Lane 3:

K562 lysate at 10 µg

Lane 4:

293T lysate at 10 µg

Lane 5:

HeLa lysate at 10 µg

Predicted band size: 141 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9845

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRP16 also known as RNA-dependent ATPase PRP16 is an essential component in pre-mRNA splicing. It functions mechanically as an ATP-dependent RNA helicase facilitating conformational changes in the spliceosome. This protein has a molecular mass of approximately 120 kDa. PRP16 expression occurs largely in the nucleus where it participates in RNA processing events.
Biological function summary

PRP16 plays a critical role in the rearrangement of spliceosomal components during the second catalytic step of splicing. It is a part of the spliceosome complex and is necessary for the accurate removal of introns from pre-mRNA. This protein ensures the proper transition of the spliceosome from the C complex to the B* complex therefore influencing mRNA maturation and stability.

Pathways

PRP16 operates within the mRNA processing and splicing pathways. It is closely related to other spliceosomal proteins such as PRP8 and SF3B1 which coordinate to ensure efficient gene expression regulation through precise splicing events. These interconnected pathways highlight PRP16’s indispensable function in gene expression and RNA management.

PRP16 exhibits a connection with genetic conditions involving splicing defects. Mutations or dysregulation of this helicase may relate to cancers or neurodegenerative diseases where splicing errors lead to aberrant protein products. PRP16's interactions with proteins like U2AF may provide additional insights into how its malfunction contributes to disease progression and pathology in such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable ATP-binding RNA helicase (Probable). Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 29301961, PubMed : 9524131).
See full target information DHX38
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 29:1111-1124 PubMed29496966

2018

C3G dynamically associates with nuclear speckles and regulates mRNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Dhruv Kumar Shakyawar,Bhattiprolu Muralikrishna,Vegesna Radha
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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