AB27692

Anti-PRP19 antibody

(5 Reviews)

(18 Publications)

Rabbit Polyclonal PRP19 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 18 publications.

View Alternative Names

NMP200, PRP19, SNEV, PRPF19, Pre-mRNA-processing factor 19, Nuclear matrix protein 200, PRP19/PSO4 homolog, RING-type E3 ubiquitin transferase PRP19, Senescence evasion factor, hPso4

2 Images

Western blot - Anti-PRP19 antibody (AB27692)

All lanes:

Western blot - Anti-PRP19 antibody (ab27692) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Project

Western blot - Anti-PRP19 antibody (AB27692)

All lanes:

Western blot - Anti-PRP19 antibody (ab27692) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Western blot - Jurkat whole cell lysate (<a href='/en-us/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>) at 20 µg

Lane 3:

Western blot - A-431 whole cell lysate (<a href='/en-us/products/cell-lysates/a-431-whole-cell-lysate-ab7909'>ab7909</a>) at 20 µg

Secondary

All lanes:

IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PRP19 protein also known as Pre-mRNA Processing factor 19 weighs approximately 55 kDa. This protein plays a role in the splicing of pre-mRNA by functioning as part of the spliceosome complex. PRP19 is expressed in many tissues and cells which reflects its important role in RNA processing. It is not confined to a specific type of tissue indicating its broad relevance in cellular processes. Mechanically PRP19 acts by enhancing the efficiency of splicing ensuring that mRNA molecules are properly matured for translation.

Biological function summary

PRP19 engages in multiple processes beyond its initial splicing role. It forms part of the NTC complex which is critical for the activation of the spliceosome. This complex includes other key proteins essential for RNA splicing transition phases. Additionally PRP19 contributes to DNA repair mechanisms playing a part in maintaining genomic integrity under stress conditions. Its role in these processes demonstrates its versatile functionality in cell homeostasis and response to damage.

Pathways

Experts recognize PRP19's involvement in the mRNA processing and DNA damage response pathways. These pathways are key to understanding how cells manage genetic information and react to physiological challenges. PRP19 interacts with other proteins such as CDC5L and PLRG1 within these pathways illustrating its interconnected role in cellular regulation. These associations underline how PRP19 integrates into critical networks that balance gene expression and genome stability.

Researchers have linked PRP19 to cancer and neurodegenerative disorders. In cancer abnormal PRP19 function may contribute to unchecked cell growth by impacting mRNA splicing and DNA repair. In neurodegenerative diseases its dysfunction might influence neuron survival through defective RNA processing. The relationship of PRP19 with proteins like BRCA1 in the context of cancer further highlights its significance in disease mechanisms. As such PRP19 serves as a potential target for therapeutic exploration.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome (PubMed : 28076346, PubMed : 28502770, PubMed : 29301961, PubMed : 29360106, PubMed : 30705154). Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex (PubMed : 20595234). Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries (PubMed : 21536736). The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair (PubMed : 17981804). Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response (PubMed : 24332808). May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA (PubMed : 18263876). As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process (PubMed : 16223718). In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation (PubMed : 11435423). May play a role in the biogenesis of lipid droplets (By similarity). May play a role in neural differentiation possibly through its function as part of the spliceosome (By similarity).

See full target information PRPF19

style

left-column

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 222: PubMed37956386

2023

Stress-induced nuclear speckle reorganization is linked to activation of immediate early gene splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Hsu-Min Sung,Johanna Schott,Philipp Boss,Janina A Lehmann,Marius Roland Hardt,Doris Lindner,Joris Messens,Ivan Bogeski,Uwe Ohler,Georg Stoecklin

Nutrients 15: PubMed37111225

2023

Extract Preventively Intervenes in Citrobacter Rodentium-Induced Colitis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Chen,Yiqiang Chen,Kaiyuan Li,Zhuo Chen,Qingyu Zhao,Yimeng Fan,Ying Liu,Suxia Zhang,Zhihui Hao

eLife 11: PubMed35880862

2022

XAB2 dynamics during DNA damage-dependent transcription inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Lise-Marie Donnio,Elena Cerutti,Charlene Magnani,Damien Neuillet,Pierre-Olivier Mari,Giuseppina Giglia-Mari

RNA (New York, N.Y.) 28:1224-1238 PubMed35768279

2022

A dual role for the RNA helicase DHX34 in NMD and pre-mRNA splicing and its function in hematopoietic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Nele Hug,Stuart Aitken,Dasa Longman,Michaela Raab,Hannah Armes,Abigail R Mann,Ana Rio-Machin,Jude Fitzgibbon,Kevin Rouault-Pierre,Javier F Cáceres

Nucleic acids research 49:5605-5622 PubMed33963872

2021

NSMF promotes the replication stress-induced DNA damage response for genome maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Min Kyung Ju,Kyeong Jin Shin,Joo Rak Lee,Keon Woo Khim,Eun A Lee,Jae Sun Ra,Byung-Gyu Kim,Han-Seul Jo,Jong Hyuk Yoon,Tae Moon Kim,Kyungjae Myung,Jang Hyun Choi,Hongtae Kim,Young Chan Chae

Nucleic acids research 49:2740-2758 PubMed33619536

2021

Hsp70 acts as a fine-switch that controls E3 ligase CHIP-mediated TAp63 and ΔNp63 ubiquitination and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

H Helena Wu,Benfan Wang,Stephen R Armstrong,Yasser Abuetabh,Sarah Leng,Wilson H Y Roa,Azeddine Atfi,Adriano Marchese,Beverly Wilson,Consolato Sergi,Elsa R Flores,David D Eisenstat,Roger P Leng

Cell death & disease 12:136 PubMed33542212

2021

A fine balance between Prpf19 and Exoc7 in achieving degradation of aggregated protein and suppression of cell death in spinocerebellar ataxia type 3.

Applications

Unspecified application

Species

Unspecified reactive species

Zhefan Stephen Chen,Xiaoying Huang,Kevin Talbot,Ho Yin Edwin Chan

Molecular cell 69:517-532.e11 PubMed29395067

2018

High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Young Youn,Wade H Dunham,Seo Jung Hong,James D R Knight,Mikhail Bashkurov,Ginny I Chen,Halil Bagci,Bhavisha Rathod,Graham MacLeod,Simon W M Eng,Stéphane Angers,Quaid Morris,Marc Fabian,Jean-François Côté,Anne-Claude Gingras

Scientific reports 7:16582 PubMed29185492

2017

Interaction profiling of RNA-binding ubiquitin ligases reveals a link between posttranscriptional regulation and the ubiquitin system.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Hildebrandt,Gregorio Alanis-Lobato,Andrea Voigt,Kathi Zarnack,Miguel A Andrade-Navarro,Petra Beli,Julian König

eLife 6: PubMed28884683

2017

Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Pawellek,Ursula Ryder,Triin Tammsalu,Lewis J King,Helmi Kreinin,Tony Ly,Ronald T Hay,Richard C Hartley,Angus I Lamond

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PRP19 antibody

Western blot - Anti-PRP19 antibody (AB27692)

Western blot - Anti-PRP19 antibody (AB27692)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise