Rabbit Polyclonal PRP19 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 17 publications.
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- Nutrients 15:2023Extract Preventively Intervenes in Citrobacter Rodentium-Induced Colitis in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Chen,Yiqiang Chen,Kaiyuan Li,Zhuo Chen,Qingyu Zhao,Yimeng Fan,Ying Liu,Suxia Zhang,Zhihui HaoPubMed 37111225
- eLife 11:2022XAB2 dynamics during DNA damage-dependent transcription inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLise-Marie Donnio,Elena Cerutti,Charlene Magnani,Damien Neuillet,Pierre-Olivier Mari,Giuseppina Giglia-MariPubMed 35880862
- RNA (New York, N.Y.) 28:1224-12382022A dual role for the RNA helicase DHX34 in NMD and pre-mRNA splicing and its function in hematopoietic differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNele Hug,Stuart Aitken,Dasa Longman,Michaela Raab,Hannah Armes,Abigail R Mann,Ana Rio-Machin,Jude Fitzgibbon,Kevin Rouault-Pierre,Javier F CáceresPubMed 35768279
- Nucleic acids research 49:5605-56222021NSMF promotes the replication stress-induced DNA damage response for genome maintenance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Kyung Ju,Kyeong Jin Shin,Joo Rak Lee,Keon Woo Khim,Eun A Lee,Jae Sun Ra,Byung-Gyu Kim,Han-Seul Jo,Jong Hyuk Yoon,Tae Moon Kim,Kyungjae Myung,Jang Hyun Choi,Hongtae Kim,Young Chan ChaePubMed 33963872
- Nucleic acids research 49:2740-27582021Hsp70 acts as a fine-switch that controls E3 ligase CHIP-mediated TAp63 and ΔNp63 ubiquitination and degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesH Helena Wu,Benfan Wang,Stephen R Armstrong,Yasser Abuetabh,Sarah Leng,Wilson H Y Roa,Azeddine Atfi,Adriano Marchese,Beverly Wilson,Consolato Sergi,Elsa R Flores,David D Eisenstat,Roger P LengPubMed 33619536
- Cell death & disease 12:1362021A fine balance between Prpf19 and Exoc7 in achieving degradation of aggregated protein and suppression of cell death in spinocerebellar ataxia type 3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhefan Stephen Chen,Xiaoying Huang,Kevin Talbot,Ho Yin Edwin ChanPubMed 33542212
- Molecular cell 69:517-532.e112018High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Young Youn,Wade H Dunham,Seo Jung Hong,James D R Knight,Mikhail Bashkurov,Ginny I Chen,Halil Bagci,Bhavisha Rathod,Graham MacLeod,Simon W M Eng,Stéphane Angers,Quaid Morris,Marc Fabian,Jean-François Côté,Anne-Claude GingrasPubMed 29395067
- Scientific reports 7:165822017Interaction profiling of RNA-binding ubiquitin ligases reveals a link between posttranscriptional regulation and the ubiquitin system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Hildebrandt,Gregorio Alanis-Lobato,Andrea Voigt,Kathi Zarnack,Miguel A Andrade-Navarro,Petra Beli,Julian KönigPubMed 29185492
- eLife 6:2017Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Pawellek et. al.PubMed 28884683
- Nucleic acids research 45:8859-88722017A phosphorylation-and-ubiquitylation circuitry driving ATR activation and homologous recombination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJean-Christophe Dubois,Maïlyn Yates,Antoine Gaudreau-Lapierre,Geneviève Clément,Laurent Cappadocia,Luc Gaudreau,Lee Zou,Alexandre MaréchalPubMed 28666352
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Chicken | Predicted |
| Xenopus laevis | Predicted |
| Zebrafish | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
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Target data
Function
Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome (PubMed:28076346, PubMed:28502770, PubMed:29301961, PubMed:29360106, PubMed:30705154). Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex (PubMed:20595234). Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries (PubMed:21536736). The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair (PubMed:17981804). Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response (PubMed:24332808). May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA (PubMed:18263876). As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process (PubMed:16223718). In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation (PubMed:11435423). May play a role in the biogenesis of lipid droplets (By similarity). May play a role in neural differentiation possibly through its function as part of the spliceosome (By similarity).
Alternative names
NMP200, PRP19, SNEV, PRPF19, Pre-mRNA-processing factor 19, Nuclear matrix protein 200, PRP19/PSO4 homolog, RING-type E3 ubiquitin transferase PRP19, Senescence evasion factor, hPso4
Recommended products
Rabbit Polyclonal PRP19 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 17 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PRP19 protein also known as Pre-mRNA Processing factor 19 weighs approximately 55 kDa. This protein plays a role in the splicing of pre-mRNA by functioning as part of the spliceosome complex. PRP19 is expressed in many tissues and cells which reflects its important role in RNA processing. It is not confined to a specific type of tissue indicating its broad relevance in cellular processes. Mechanically PRP19 acts by enhancing the efficiency of splicing ensuring that mRNA molecules are properly matured for translation.
- Biological function summary
PRP19 engages in multiple processes beyond its initial splicing role. It forms part of the NTC complex which is critical for the activation of the spliceosome. This complex includes other key proteins essential for RNA splicing transition phases. Additionally PRP19 contributes to DNA repair mechanisms playing a part in maintaining genomic integrity under stress conditions. Its role in these processes demonstrates its versatile functionality in cell homeostasis and response to damage.
- Pathways
Experts recognize PRP19's involvement in the mRNA processing and DNA damage response pathways. These pathways are key to understanding how cells manage genetic information and react to physiological challenges. PRP19 interacts with other proteins such as CDC5L and PLRG1 within these pathways illustrating its interconnected role in cellular regulation. These associations underline how PRP19 integrates into critical networks that balance gene expression and genome stability.
- Associated diseases and disorders
Researchers have linked PRP19 to cancer and neurodegenerative disorders. In cancer abnormal PRP19 function may contribute to unchecked cell growth by impacting mRNA splicing and DNA repair. In neurodegenerative diseases its dysfunction might influence neuron survival through defective RNA processing. The relationship of PRP19 with proteins like BRCA1 in the context of cancer further highlights its significance in disease mechanisms. As such PRP19 serves as a potential target for therapeutic exploration.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PRP19 antibody (ab27692)
All lanes: Western blot - Anti-PRP19 antibody (ab27692) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Western blot - Anti-PRP19 antibody (ab27692)
All lanes: Western blot - Anti-PRP19 antibody (ab27692) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
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