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Rabbit Recombinant Monoclonal PRP19 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

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Images

Western blot - Anti-PRP19 antibody [EPR7446] (AB126776), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] (AB126776), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] (AB126776), expandable thumbnail
  • OI-RD Scanning - Anti-PRP19 antibody [EPR7446] (AB126776), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Tested
Rat
Expected
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome (PubMed:28502770, PubMed:28076346, PubMed:29360106, PubMed:29301961, PubMed:30705154). Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex (PubMed:20595234). Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries (PubMed:21536736). The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair (PubMed:17981804). Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response (PubMed:24332808). May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA (PubMed:18263876). As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process (PubMed:16223718). In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation (PubMed:11435423). May play a role in the biogenesis of lipid droplets (By similarity). May play a role in neural differentiation possibly through its function as part of the spliceosome (By similarity).

Alternative names

Pre-mRNA-processing factor 19, Nuclear matrix protein 200, PRP19/PSO4 homolog, RING-type E3 ubiquitin transferase PRP19, Senescence evasion factor, hPso4, PRPF19, SNEV, NMP200, PRP19

Recommended products

Rabbit Recombinant Monoclonal PRP19 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR7446

Purification technique

Affinity purification Protein A

Dissociation constant

1.06 x 10-10 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The PRP19 protein also known as Pre-mRNA Processing factor 19 weighs approximately 55 kDa. This protein plays a role in the splicing of pre-mRNA by functioning as part of the spliceosome complex. PRP19 is expressed in many tissues and cells which reflects its important role in RNA processing. It is not confined to a specific type of tissue indicating its broad relevance in cellular processes. Mechanically PRP19 acts by enhancing the efficiency of splicing ensuring that mRNA molecules are properly matured for translation.

Biological function summary

PRP19 engages in multiple processes beyond its initial splicing role. It forms part of the NTC complex which is critical for the activation of the spliceosome. This complex includes other key proteins essential for RNA splicing transition phases. Additionally PRP19 contributes to DNA repair mechanisms playing a part in maintaining genomic integrity under stress conditions. Its role in these processes demonstrates its versatile functionality in cell homeostasis and response to damage.

Pathways

Experts recognize PRP19's involvement in the mRNA processing and DNA damage response pathways. These pathways are key to understanding how cells manage genetic information and react to physiological challenges. PRP19 interacts with other proteins such as CDC5L and PLRG1 within these pathways illustrating its interconnected role in cellular regulation. These associations underline how PRP19 integrates into critical networks that balance gene expression and genome stability.

Associated diseases and disorders

Researchers have linked PRP19 to cancer and neurodegenerative disorders. In cancer abnormal PRP19 function may contribute to unchecked cell growth by impacting mRNA splicing and DNA repair. In neurodegenerative diseases its dysfunction might influence neuron survival through defective RNA processing. The relationship of PRP19 with proteins like BRCA1 in the context of cancer further highlights its significance in disease mechanisms. As such PRP19 serves as a potential target for therapeutic exploration.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-PRP19 antibody [EPR7446] (ab126776), expandable thumbnail

    Western blot - Anti-PRP19 antibody [EPR7446] (ab126776)

    All lanes: Western blot - Anti-PRP19 antibody [EPR7446] (ab126776) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: A431 cell lysate at 10 µg

    Lane 3: MCF7 cell lysate at 10 µg

    Lane 4: PC-12 cell lysate at 10 µg

    Lane 5: NIH/3T3 cell lysate at 10 µg

    Lane 6: HeLa cell lysate at 10 µg

    Lane 7: K562 cell lysate at 10 µg

    Lane 8: U937 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] (ab126776), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] (ab126776)

    ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] (ab126776), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] (ab126776)

    ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-PRP19 antibody [EPR7446] (ab126776), expandable thumbnail

    OI-RD Scanning - Anti-PRP19 antibody [EPR7446] (ab126776)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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