Rabbit Polyclonal PRP6/ANT-1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PRPF6 aa 1-50.
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Publications
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- Oncology research 29:275-2892023PRPF6 promotes metastasis and paclitaxel resistance of ovarian cancer via SNHG16/CEBPB/GATA3 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan Wang,Yingying Zhou,Siyang Zhang,Y A Qi,Min WangPubMed 37303939
- Nucleic acids research 50:5263-52812022Reovirus μ2 protein modulates host cell alternative splicing by reducing protein levels of U5 snRNP core components.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimon Boudreault,Mathieu Durand,Carole-Anne Martineau,Jean-Pierre Perreault,Guy Lemay,Martin BisaillonPubMed 35489070
- Nature communications 8:156152017R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein.Applications:WBReactive species:Unspecified reactive speciesPhilippe Cloutier et. al.PubMed 28561026
- PLoS pathogens 10:e10041992014Cytoplasmic viral RNA-dependent RNA polymerase disrupts the intracellular splicing machinery by entering the nucleus and interfering with Prp8.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PRPF6 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O94906
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Platypus | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Guinea pig, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus, Cow | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Guinea pig, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Guinea pig, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus, Cow | Dilution info - | Notes - |
Target data
Function
Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed:20118938, PubMed:21549338, PubMed:28781166). Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.
Alternative names
C20orf14, PRPF6, Pre-mRNA-processing factor 6, Androgen receptor N-terminal domain-transactivating protein 1, PRP6 homolog, U5 snRNP-associated 102 kDa protein, ANT-1, U5-102 kDa protein
Recommended products
Rabbit Polyclonal PRP6/ANT-1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PRPF6 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PRPF6 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O94906
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PRP6 also known as ANT-1 is a protein involved in pre-mRNA splicing. Its molecular mass is approximately 111 kDa. PRP6 is expressed ubiquitously across different tissues but shows higher expression in cells with high transcriptional activity. It functions as a part of the spliceosome a large complex responsible for the removal of introns from pre-mRNA. The protein's structure consists of distinct domains that mediate interactions with other spliceosomal components which ensures proper assembly and function.
- Biological function summary
This protein has a significant role in regulating gene expression at the post-transcriptional level. PRP6 forms part of the U4/U6-U5 tri-snRNP complex in the spliceosome. This complex plays an important role during spliceosome assembly and the activation process. By facilitating accurate splicing PRP6 influences the diversity of proteins produced by cells. It interacts directly with other proteins like ANT-Anti which contribute to its function.
- Pathways
Several key processes involve PRP6's function. It integrates into the splicing pathway which is essential for mRNA maturation. PRP6 is related to proteins like ANT1s which regulates energy metabolism and homeostasis. In addition PRP6 participates in pathways that control the cellular response to different growth factors and environmental signals. This role links PRP6 to the regulation of other important cellular functions including cell growth and differentiation.
- Associated diseases and disorders
Research indicates that impairment in PRP6 function may associate with certain types of cancer and splicing-related genetic disorders. Altered splicing patterns often lead to oncogenic transformations connecting PRP6 to cancer development through pathways involving proteins such as ANT-Anti. Moreover mutations or malfunctions in splicing factors like PRP6 can result in various neurodegenerative diseases highlighting its critical role in maintaining transcriptome integrity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-PRP6/ANT-1 antibody (ab99292)
All lanes: Western blot - Anti-PRP6/ANT-1 antibody (ab99292) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Lane 5: NIH3T3 whole cell lysate at 50 µg
Predicted band size: 106 kDa
Exposure time: 30s
Immunoprecipitation - Anti-PRP6/ANT-1 antibody (ab99292)
Lane 1 : Detection of Human PRP6/ANT-1 by immunoprecipitation from HeLa (1 mg for IP, 20% of IP loaded), using 3μg/ml ab99292. Lane 2: Control IgG
All lanes: Immunoprecipitation - Anti-PRP6/ANT-1 antibody (ab99292)
Predicted band size: 106 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP6/ANT-1 antibody (ab99292)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PRP6 with ab99292 at 1/200 (1µg/ml). Detection: DAB.
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