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AB272596

Anti-PRPF3 antibody

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Rabbit Polyclonal PRPF3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PRPF3 aa 350-500.

View Alternative Names

HPRP3, PRP3, PRPF3, U4/U6 small nuclear ribonucleoprotein Prp3, Pre-mRNA-splicing factor 3, U4/U6 snRNP 90 kDa protein, hPrp3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PRPF3 antibody (AB272596)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PRPF3 antibody (AB272596)

PFA fixed, Triton X-100 permeablized human cell line A431 labeling PRPF3 (green) using ab272596 at 2 ug/ml in ICC/IF analysis.

Western blot - Anti-PRPF3 antibody (AB272596)
  • WB

Unknown

Western blot - Anti-PRPF3 antibody (AB272596)

All lanes:

Western blot - Anti-PRPF3 antibody (ab272596) at 0.4 µg/mL

Lane 1:

Control (vector only transfected HEK-293T lysate)

Lane 2:

PRPF3 over-expression lysate in mammalian HEK-293T cells

Predicted band size: 78 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF3 antibody (AB272596)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF3 antibody (AB272596)

Paraffin-embedded formalin-fixed Human stomach tissue stained for PRPF3 using ab272596 at 1/200 in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PRPF3 aa 350-500. The exact immunogen used to generate this antibody is proprietary information.

O43395

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRPF3 also known as Pre-mRNA processing factor 3 plays an important role in the spliceosome machinery necessary for the splicing of pre-mRNA. It is part of the U4/U6-U5 tri-snRNP complex which is important for the proper assembly of the spliceosome. This protein weighs approximately 77 kDa and can be found in the nucleus of cells where mRNA splicing takes place. Its expression occurs across various tissues reflecting its relevance in general cellular functions.
Biological function summary

PRPF3 is involved in mRNA processing by facilitating the removal of introns from pre-mRNA transcripts. In its role within the spliceosome it works alongside other proteins in the complex to ensure accurate intron excision and exon ligation. This process is essential for creating mature and functional mRNA which translates into proteins vital for cellular operations. PRPF3 ensures the precision of RNA splicing affecting gene expression regulation and influencing alternative splicing events.

Pathways

PRPF3 is integrated into the spliceosomal cycle and is essential for the regulation of gene expression. This protein interacts with other spliceosome-associated proteins such as PRPF8 and SNRNP200 participating in the assembly and activation of the spliceosome. Its function influences the mRNA surveillance pathway ensuring only properly spliced mRNAs are translated into proteins which is a critical regulatory mechanism in cells.

Mutations in the PRPF3 gene have been linked to Retinitis Pigmentosa 18 a genetic disorder leading to progressive vision loss. The dysfunction can impact the interactions of PRPF3 with other spliceosomal proteins such as PRPF8 disrupting normal RNA splicing and leading to photoreceptor cell death. Additionally aberrant splicing events involving PRPF3 can contribute to other genetic conditions where precise splicing is impaired highlighting its importance in maintaining cellular homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).
See full target information PRPF3
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