Rabbit Polyclonal PRPF31 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human U4/U6 small nuclear ribonucleoprotein Prp31 aa 200 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Mouse | Predicted |
Rat | Predicted |
Cow | Predicted |
Rhesus monkey | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Rhesus monkey | Dilution info - | Notes - |
Select an associated product type
Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11867543, PubMed:20118938, PubMed:28781166). Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed:11867543).
PRP31, PRP31, PRPF31, U4/U6 small nuclear ribonucleoprotein Prp31, Pre-mRNA-processing factor 31, Serologically defined breast cancer antigen NY-BR-99, U4/U6 snRNP 61 kDa protein, Protein 61K, hPrp31
Rabbit Polyclonal PRPF31 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human U4/U6 small nuclear ribonucleoprotein Prp31 aa 200 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This supplementary information is collated from multiple sources and compiled automatically.
PRPF31 also known as pre-mRNA processing factor 31 is an important component of the spliceosomal complex. It weights approximately 56 kDa. This protein is expressed in various tissues with prominence in the retina. It plays a critical role in the assembly of the spliceosome by interacting with other splicing factors essential for processing pre-mRNA into mature mRNA.
PRPF31 is involved in RNA splicing a process fundamental for gene expression. It forms part of the U4/U6-U5 snRNP complex which is responsible for catalyzing the splicing of pre-mRNA. PRPF31 facilitates the rearrangement of RNA and protein components within the spliceosome ensuring accurate splicing and removal of introns.
The protein plays a role in the spliceosome pathway and genetic information processing. PRPF31 interacts with other splicing factors like PRPF6 and SNRNP200 within these pathways. Its activity is important for maintaining the fidelity and efficiency of spliceosome assembly and function linking gene regulation and expression to various cellular responses.
Mutations in PRPF31 lead to autosomal dominant retinitis pigmentosa a progressive retinal disorder. This condition connects PRPF31 to other retinal proteins like RP9. Its dysfunction disrupts normal splicing activities causing photoreceptor cell degeneration and vision loss. Understanding PRPF31's role provides insight into therapeutic targets for retinal diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7.5 % SDS-PAGE
All lanes: Western blot - Anti-PRPF31 antibody (ab184127) at 1/1000 dilution
Lane 1: A549 cell lysate
Lane 2: H1299 cell lysate
Lane 3: HCT116 whole cell lysate
Predicted band size: 55 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com