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Rabbit Polyclonal PRPF31 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human U4/U6 small nuclear ribonucleoprotein Prp31 aa 200 to C-terminus.

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Images

Western blot - Anti-PRPF31 antibody (AB184127), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human U4/U6 small nuclear ribonucleoprotein Prp31 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WWY3

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted
Cow
Predicted
Rhesus monkey
Predicted

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Cow, Rhesus monkey

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11867543, PubMed:20118938, PubMed:28781166). Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed:11867543).

Alternative names

Recommended products

Rabbit Polyclonal PRPF31 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human U4/U6 small nuclear ribonucleoprotein Prp31 aa 200 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human U4/U6 small nuclear ribonucleoprotein Prp31 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WWY3
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PRPF31 also known as pre-mRNA processing factor 31 is an important component of the spliceosomal complex. It weights approximately 56 kDa. This protein is expressed in various tissues with prominence in the retina. It plays a critical role in the assembly of the spliceosome by interacting with other splicing factors essential for processing pre-mRNA into mature mRNA.

Biological function summary

PRPF31 is involved in RNA splicing a process fundamental for gene expression. It forms part of the U4/U6-U5 snRNP complex which is responsible for catalyzing the splicing of pre-mRNA. PRPF31 facilitates the rearrangement of RNA and protein components within the spliceosome ensuring accurate splicing and removal of introns.

Pathways

The protein plays a role in the spliceosome pathway and genetic information processing. PRPF31 interacts with other splicing factors like PRPF6 and SNRNP200 within these pathways. Its activity is important for maintaining the fidelity and efficiency of spliceosome assembly and function linking gene regulation and expression to various cellular responses.

Associated diseases and disorders

Mutations in PRPF31 lead to autosomal dominant retinitis pigmentosa a progressive retinal disorder. This condition connects PRPF31 to other retinal proteins like RP9. Its dysfunction disrupts normal splicing activities causing photoreceptor cell degeneration and vision loss. Understanding PRPF31's role provides insight into therapeutic targets for retinal diseases.

Product promise

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1 product image

  • Western blot - Anti-PRPF31 antibody (ab184127), expandable thumbnail

    Western blot - Anti-PRPF31 antibody (ab184127)

    7.5 % SDS-PAGE

    All lanes: Western blot - Anti-PRPF31 antibody (ab184127) at 1/1000 dilution

    Lane 1: A549 cell lysate

    Lane 2: H1299 cell lysate

    Lane 3: HCT116 whole cell lysate

    Predicted band size: 55 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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