AB188577

Anti-PRPF31 antibody [EPR14587]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal PRPF31 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 7 publications.

View Alternative Names

PRP31, PRPF31, U4/U6 small nuclear ribonucleoprotein Prp31, Pre-mRNA-processing factor 31, Serologically defined breast cancer antigen NY-BR-99, U4/U6 snRNP 61 kDa protein, Protein 61K, hPrp31

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-PRPF31 antibody [EPR14587] (AB188577)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PRPF31 antibody [EPR14587] (AB188577)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling PRPF31 with ab188577 at a 1/250 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.

Flow Cytometry (Intracellular) - Anti-PRPF31 antibody [EPR14587] (AB188577)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PRPF31 antibody [EPR14587] (AB188577)

Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling PRPF31 with ab188577 at a 1/240 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

Supplier Data

Western blot - Anti-PRPF31 antibody [EPR14587] (AB188577)

All lanes:

Western blot - Anti-PRPF31 antibody [EPR14587] (ab188577) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

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Supplier Data

Western blot - Anti-PRPF31 antibody [EPR14587] (AB188577)

All lanes:

Western blot - Anti-PRPF31 antibody [EPR14587] (ab188577) at 1/1000 dilution

All lanes:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

true

CiteAb

Western blot - Anti-PRPF31 antibody [EPR14587] (AB188577)

PRPF31 western blot using anti-PRPF31 antibody [EPR14587] ab188577. Publication image and figure legend from Zuo, S., Li, X., et al., 2020, Sci Rep, PubMed 32483193.

ab188577 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab188577 please see the product overview.

The expression of PRP3 is increased in cSCC cells and tissues. (A) PRP3 mRNA expression in cSCC cells compared with benign epidermal keratinocyte cells. (B) PRP3 protein expression in cSCC cells compared with benign epidermal keratinocyte cells. (C) Relative protein expression of PRP3 in cSCC cells versus benign epidermal keratinocyte cells. (D) PRP3 protein expression in cSCC tissues was examined via western-blot. (E) PRP3 protein expression in normal human skin, AK, cSCCs in situ and sporadic cSCCs tissues were explored via IHC. (F) High PRP3 expression is associated with poor prognosis in cSCCs patients. *p < 0.05, **p < 0.01. PRP3, pre-mRNA processing factor 3.

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14587

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/240", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRPF31 also known as pre-mRNA processing factor 31 is an important component of the spliceosomal complex. It weights approximately 56 kDa. This protein is expressed in various tissues with prominence in the retina. It plays a critical role in the assembly of the spliceosome by interacting with other splicing factors essential for processing pre-mRNA into mature mRNA.

Biological function summary

PRPF31 is involved in RNA splicing a process fundamental for gene expression. It forms part of the U4/U6-U5 snRNP complex which is responsible for catalyzing the splicing of pre-mRNA. PRPF31 facilitates the rearrangement of RNA and protein components within the spliceosome ensuring accurate splicing and removal of introns.

Pathways

The protein plays a role in the spliceosome pathway and genetic information processing. PRPF31 interacts with other splicing factors like PRPF6 and SNRNP200 within these pathways. Its activity is important for maintaining the fidelity and efficiency of spliceosome assembly and function linking gene regulation and expression to various cellular responses.

Mutations in PRPF31 lead to autosomal dominant retinitis pigmentosa a progressive retinal disorder. This condition connects PRPF31 to other retinal proteins like RP9. Its dysfunction disrupts normal splicing activities causing photoreceptor cell degeneration and vision loss. Understanding PRPF31's role provides insight into therapeutic targets for retinal diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11867543, PubMed : 20118938, PubMed : 28781166). Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed : 11867543).

See full target information PRPF31

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 15:e17973 PubMed37800682

2023

Trichothiodystrophy-associated MPLKIP maintains DBR1 levels for proper lariat debranching and ectodermal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Arjan F Theil,Alex Pines,Tuğba Kalayci,José M Heredia-Genestar,Anja Raams,Marion H Rietveld,Sriram Sridharan,Sabine Ej Tanis,Klaas W Mulder,Nesimi Büyükbabani,Birsen Karaman,Zehra O Uyguner,Hülya Kayserili,Jan Hj Hoeijmakers,Hannes Lans,Jeroen Aa Demmers,Joris Pothof,Umut Altunoglu,Abdoelwaheb El Ghalbzouri,Wim Vermeulen

Nature communications 12:3646 PubMed34131137

2021

TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation.

Applications

Unspecified application

Species

Unspecified reactive species

Klára Klimešová,Jitka Vojáčková,Nenad Radivojević,Franck Vandermoere,Edouard Bertrand,Celine Verheggen,David Staněk

Scientific reports 10:8863 PubMed32483193

2020

Pre-mRNA processing factor 3 enhances the progression of keratinocyte-derived cutaneous squamous cell carcinoma by regulating the JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Siyao Zuo,Xin Li,Wanguo Bao,Shanshan Li

OncoTargets and therapy 13:4717-4730 PubMed32547101

2020

Pre-mRNA Processing Factor 8 Accelerates the Progression of Hepatocellular Carcinoma by Regulating the PI3K/Akt Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shouhan Wang,Min Wang,Bin Wang,Jiaqi Chen,Xianbin Cheng,Xiaodan Sun

Archives of biochemistry and biophysics 677:108159 PubMed31669268

2019

Thioredoxin interacting protein (Txnip) forms redox sensitive high molecular weight nucleoprotein complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Cristiane Lumi Hirata,Shinji Ito,Hiroshi Masutani

Journal of cellular biochemistry 119:6775-6783 PubMed29744916

2018

Human organotypic retinal flat-mount culture (HORFC) as a model for retinitis pigmentosa11.

Applications

Unspecified application

Species

Unspecified reactive species

Leila Azizzadeh Pormehr,Narsis Daftarian,Shahin Ahmadian,Mozhgan Rezaei Kanavi,Hamid Ahmadieh,Mahshid Shafiezadeh

Nucleic acids research 46:3774-3790 PubMed29415178

2018

The Sm-core mediates the retention of partially-assembled spliceosomal snRNPs in Cajal bodies until their full maturation.

Applications

Unspecified application

Species

Human

Adriana Roithová,Klára Klimešová,Josef Pánek,Cindy L Will,Reinhard Lührmann,David Stanek,Cyrille Girard

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PRPF31 antibody [EPR14587]

Immunocytochemistry/ Immunofluorescence - Anti-PRPF31 antibody [EPR14587] (AB188577)

Flow Cytometry (Intracellular) - Anti-PRPF31 antibody [EPR14587] (AB188577)

Western blot - Anti-PRPF31 antibody [EPR14587] (AB188577)

Western blot - Anti-PRPF31 antibody [EPR14587] (AB188577)

Western blot - Anti-PRPF31 antibody [EPR14587] (AB188577)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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