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Rabbit Recombinant Monoclonal PRPF8/Prp8 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347), expandable thumbnail
  • Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347), expandable thumbnail
  • Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome (PubMed:10411133, PubMed:11971955, PubMed:28076346, PubMed:28502770, PubMed:28781166, PubMed:29301961, PubMed:29360106, PubMed:29361316, PubMed:30315277, PubMed:30705154, PubMed:30728453). Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.

Alternative names

PRPC8, PRPF8, Pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, Splicing factor Prp8, p220

Recommended products

Rabbit Recombinant Monoclonal PRPF8/Prp8 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15228
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347), expandable thumbnail

    Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347)

    All lanes: Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347) at 1/5000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: K562 cell lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 274 kDa

    Observed band size: 274 kDa

  • Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347), expandable thumbnail

    Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347)

    All lanes: Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347) at 1/20000 dilution

    All lanes: 293T cell lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 274 kDa

    Observed band size: 274 kDa

  • Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347), expandable thumbnail

    Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347)

    All lanes: Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347) at 1/1000 dilution

    Lane 1: mouse RAW 264.7 cell lysate at 10 µg

    Lane 2: rat PC12 cell lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 274 kDa

    Observed band size: 274 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347)

    Immunohistochemical analysis of paraffin-embedded, Human ovarian carcinoma tissue labeling PRPF8/Prp8 with ab190347 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347)

    Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling PRPF8/Prp8 with ab190347 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com