Rabbit Recombinant Monoclonal PRPF8/Prp8 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (AB239003), expandable thumbnail](https://content.abcam.com/products/images/prpf8-prp8-antibody-epr15229-bsa-and-azide-free-ab239003--immunocytochemistry-immunofluorescence-img69540.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (AB239003)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (AB239003), expandable thumbnail](https://content.abcam.com/products/images/prpf8-prp8-antibody-epr15229-bsa-and-azide-free-ab239003--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img37127.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (AB239003)")
![Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (AB239003), expandable thumbnail](https://content.abcam.com/products/images/prpf8-prp8-antibody-epr15229-bsa-and-azide-free-ab239003--flow-cytometry-intracellular-img50443.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (AB239003)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (AB239003), expandable thumbnail](https://content.abcam.com/products/images/prpf8-prp8-antibody-epr15229-bsa-and-azide-free-ab239003--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58005.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (AB239003)")
![Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (AB239003), expandable thumbnail](https://content.abcam.com/products/images/prpf8-prp8-antibody-epr15229-bsa-and-azide-free-ab239003--immunocytochemistry-immunofluorescence-img96033.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (AB239003)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Mouse | Expected | Predicted | Predicted | Predicted |
| Rat | Expected | Predicted | Predicted | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome (PubMed:10411133, PubMed:11971955, PubMed:28076346, PubMed:28502770, PubMed:28781166, PubMed:29301961, PubMed:29360106, PubMed:29361316, PubMed:30315277, PubMed:30705154, PubMed:30728453). Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.
Alternative names
PRPC8, PRPF8, Pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, Splicing factor Prp8, p220
Recommended products
Rabbit Recombinant Monoclonal PRPF8/Prp8 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR15229
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab239003 is the carrier-free version of Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003), expandable thumbnail](https://content.abcam.com/products/images/prpf8-prp8-antibody-epr15229-bsa-and-azide-free-ab239003--immunocytochemistry-immunofluorescence-img69540.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)")
Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
Immunocytochemistry/ Immunofluorescence analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling PRPF8/Prp8 with Purified Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547 at 1:100 dilution (10 μg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003), expandable thumbnail](https://content.abcam.com/products/images/prpf8-prp8-antibody-epr15229-bsa-and-azide-free-ab239003--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img37127.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric cancer tissue sections labeling PRPF8/Prp8 with Purified Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547 at 1:2000 dilution (0.52 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547).
![Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003), expandable thumbnail](https://content.abcam.com/products/images/prpf8-prp8-antibody-epr15229-bsa-and-azide-free-ab239003--flow-cytometry-intracellular-img50443.jpg "Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)")
Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
Intracellular Flow Cytometry analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling PRPF8/Prp8 with Purified Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547 at 1/100 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003), expandable thumbnail](https://content.abcam.com/products/images/prpf8-prp8-antibody-epr15229-bsa-and-azide-free-ab239003--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58005.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
Immunohistochemical analysis of paraffin embedded Human cervix carcinoma tissue sections labeling PRPF8/Prp8 using unpurifed Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547).
![Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003), expandable thumbnail](https://content.abcam.com/products/images/prpf8-prp8-antibody-epr15229-bsa-and-azide-free-ab239003--immunocytochemistry-immunofluorescence-img96033.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)")
Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
Immunofluorescent analysis of formaldehyde fixed HeLa cells labeling PRPF8/Prp8 using unpurified Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547).
![Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003), expandable thumbnail](https://content.abcam.com/products/images/prpf8-prp8-antibody-epr15229-bsa-and-azide-free-ab239003--flow-cytometry-intracellular-img66033.jpg "Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)")
Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
Intracellular Flow Cytometry analysis of K562 cells labeling PRPF8/Prp8 using unpurified Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547 at a 1/190 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal ab185547).
![Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003), expandable thumbnail](https://content.abcam.com/products/images/prpf8-prp8-antibody-epr15229-bsa-and-azide-free-ab239003--flow-cytometry-intracellular-img66033.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)")
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