Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (AB185547)

Immunohistochemical analysis of paraffin embedded Human cervix carcinoma tissue sections labeling PRPF8/Prp8 using unpurifed ab185547 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (AB185547)

Immunocytochemistry/ Immunofluorescence analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling PRPF8/Prp8 with Purified ab185547 at 1 : 100 dilution (10 μg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (AB185547)

Intracellular Flow Cytometry analysis of K562 cells labeling PRPF8/Prp8 using unpurified ab185547 at a 1/190 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (AB185547)

Immunofluorescent analysis of formaldehyde fixed HeLa cells labeling PRPF8/Prp8 using unpurified ab185547 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (AB185547)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric cancer tissue sections labeling PRPF8/Prp8 with Purified ab185547 at 1 : 2000 dilution (0.52 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (AB185547)

Intracellular Flow Cytometry analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling PRPF8/Prp8 with Purified ab185547 at 1/100 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• WB

Supplier Data

Western blot - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (AB185547)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (ab185547) at 1/20000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 274 kDa

Observed band size: 274 kDa

false

• WB

Unknown

Western blot - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (AB185547)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Anti-Ikaros antibody [SP108] (<a href='/en-us/products/unavailable/ikaros-antibody-sp108-ab188547'>ab188547</a>) at 0.01 µg/mL

Lane 1:

C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 274 kDa

false