Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal

8 product images

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  Western blot - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (ab185547)

  Blocking/ Dilution buffer: 5% NFDM /TBST.

  All lanes: Western blot - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (ab185547) at 1/20000 dilution

  Lane 1: K562 cell lysate at 20 µg

  Lane 2: 293T cell lysate at 20 µg

  Lane 3: HeLa cell lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

  Predicted band size: 274 kDa

  Observed band size: 274 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (ab185547)

  Immunocytochemistry/ Immunofluorescence analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling PRPF8/Prp8 with Purified ab185547 at 1:100 dilution (10 μg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (ab185547)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric cancer tissue sections labeling PRPF8/Prp8 with Purified ab185547 at 1:2000 dilution (0.52 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

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  Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (ab185547)

  Intracellular Flow Cytometry analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling PRPF8/Prp8 with Purified ab185547 at 1/100 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  

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  Western blot - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (ab185547)

  Blocking and diluting buffer: 5% NFDM/TBST

  All lanes: Anti-Ikaros antibody [SP108] (ab188547) at 0.01 µg/mL

  Lane 1: C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

  Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

  Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysates at 15 µg

  Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 274 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (ab185547)

  Immunohistochemical analysis of paraffin embedded Human cervix carcinoma tissue sections labeling PRPF8/Prp8 using unpurifed ab185547 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

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  Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (ab185547)

  Immunofluorescent analysis of formaldehyde fixed HeLa cells labeling PRPF8/Prp8 using unpurified ab185547 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

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  Flow Cytometry (Intracellular) - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (ab185547)

  Intracellular Flow Cytometry analysis of K562 cells labeling PRPF8/Prp8 using unpurified ab185547 at a 1/190 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.