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AB308202

Anti-PRRS virus Nucleocapsid protein antibody [HL1901]

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(1 Publication)

Rabbit Recombinant Monoclonal PRRS virus Nucleocapsid protein antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Recombinant fragment - PRRSV samples. Cited in 1 publication.
3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PRRS virus Nucleocapsid protein antibody [HL1901] (AB308202)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PRRS virus Nucleocapsid protein antibody [HL1901] (AB308202)

For immunofluorescence analysis, mock and transfected HEK-293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of PRRS virus Nucleocapsid protein using ab308202 at 1/500 dilution (green)

Nuclei (blue) is stained with fluoroshield with DAPI.

Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL1901] (AB308202)
  • WB

Supplier Data

Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL1901] (AB308202)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS virus Nucleocapsid protein antibody [HL1901] (ab308202) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL1901] (ab308202) at 1/1000 dilution

Lane 1:

Non-transfected (–) HEK-293T whole cell lysate at 30 µg

Lane 2:

Transfected (+) HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL1901] (AB308202)
  • WB

Supplier Data

Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL1901] (AB308202)

Non-infected (–) and infected (+) Marc-145 whole cell extracts (5 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS virus Nucleocapsid protein antibody [HL1901] (ab308202) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL1901] (ab308202) at 1/1000 dilution

Lane 1:

Non-infected (–) Marc-145 whole cell lysate at 5 µg

Lanes 2 - 3:

Infected (+) Marc-145 whole cell lysate at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1901

Isotype

IgG

Carrier free

Yes

Reacts with

PRRSV

Applications

ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "PRRSV": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - PRRSV": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information PRRS virus Nucleocapsid protein
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 11:1428273 PubMed39568484

2024

Histopathological characteristics of PRRS and expression profiles of viral receptors in the piglet immune system.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Chen,Na Chen,Hongbo Chen,Zefang Zhao,Jiayao Yang,Jianbo Sun,Hanmei Li,Rihua Cong,Hailong Liu,Tengfei Liu,Shulin Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com